HTR2B 蛋白, Human (Cell-Free, His, SUMO)

The HTR2B protein is a multifunctional G protein-coupled receptor for serotonin and ergot alkaloids that undergoes conformational changes upon ligand binding, initiating signaling through G proteins and β-arrestin. This cascade involves the phosphatidylinositol-calcium second messenger system, regulating phosphatidylinositol 3-kinase activity and Ca(2+) release. HTR2B Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived HTR2B protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of HTR2B Protein, Human (Cell-Free, His, SUMO) is 481 a.a., with molecular weight of 70.3 kDa.

HTR2B 蛋白作为 5-羟色胺（血清素）和各种麦角生物碱衍生物以及精神活性物质的多功能 G 蛋白偶联受体。配体结合后，HTR2B 会发生构象变化，通过鸟嘌呤核苷酸结合蛋白（G 蛋白）启动信号传导，影响下游效应器。同时，β-抑制蛋白家族的成员抑制 G 蛋白信号传导并激活替代途径。这种复杂的信号级联进一步涉及磷脂酰肌醇-钙第二信使系统，调节磷脂酰肌醇3-激酶活性、下游信号级联，并促进细胞内储存的Ca(2+)离子的释放。HTR2B 在调节多巴胺和 5-羟色胺的释放、摄取和细胞外水平方面发挥着至关重要的作用，从而影响神经活动并可能促进疼痛感知。它的参与延伸到行为的调节，包括冲动行为。此外，HTR2B对于正常胚胎心肌细胞增殖、心脏发育、防止心肌细胞凋亡、肺动脉适应慢性缺氧、血管收缩、正常成骨细胞功能和增殖以及骨密度的维持是不可或缺的。它还在肠道内 Cajal 间质细胞的增殖中发挥着至关重要的作用，有记录表明其 C 末端与 MPDZ 存在相互作用。

Human

E. coli Cell-free

N-6*His;N-SUMO

P41595 (M1-V481)

3357

MALSYRVSELQSTIPEHILQSTFVHVISSNWSGLQTESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIMHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKKAYLVKNKPPQRLTWLTVSTVFQRDETPCSSPEKVAMLDGSRKDKALPNSGDETLMRRTSTIGKKSVQTISNEQRASKVLGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTLRKRSSKIYFRNPMAENSKFFKKHGIRNGINPAMYQSPMRLRSSTIQSSSIILLDTLLLTENEGDKTEEQVSYV

70.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.