HTRA2/OMI 蛋白, Human (HEK293, His)

HTRA2/OMI, a serine protease, crucially induces cell death via multiple mechanisms. It directly inhibits BIRC proteins, intensifying caspase activity, and can also induce cell death through a BIRC-independent, caspase-independent pathway, relying on its serine protease activity. Additionally, during apoptosis, it cleaves THAP5, promoting its degradation. Notably, isoform 2 lacks apparent proteolytic activity. HTRA2/OMI Protein, Human (HEK293, His) is the recombinant human-derived HTRA2/OMI protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HTRA2/OMI Protein, Human (HEK293, His) is 325 a.a., with molecular weight of 39-43 kDa.

HTRA2/OMI 是一种丝氨酸蛋白酶，对非特异性底物 β-酪蛋白表现出蛋白水解活性。它通过多种机制在细胞死亡诱导中发挥关键作用。一种机制涉及直接结合和抑制 BIRC 蛋白（凋亡蛋白抑制剂，IAP），从而导致 caspase 活性增强。或者，HTRA2/OMI 可以通过 BIRC 抑制独立、半胱天冬酶独立途径（依赖于其丝氨酸蛋白酶活性）诱导细胞死亡。此外，在细胞凋亡过程中，它会裂解 THAP5，促进其降解。值得注意的是，异构体 2 似乎缺乏蛋白水解活性。

Human

HEK293

C-6*His

O43464 (A134-E458)

27429  [NCBI]

AVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE

39-43 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.