1. 重组蛋白
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  3. Interleukin & Receptors Neurotrophic Factors
  4. IL-1
  5. IL-1 alpha
  6. IL-1 alpha 蛋白, Human (HEK293)

IL-1 alpha 蛋白, Human (HEK293)

目录号: HY-P73145
COA 产品使用指南

IL-1 alpha 是一种普遍存在的关键促炎细胞因子。IL-1 alpha 由单核细胞和巨噬细胞作为前蛋白产生,它经过蛋白水解加工并响应细胞损伤而释放,从而诱导细胞凋亡。IL-1 alpha 在炎症中发挥重要作用,并连接先天免疫和适应性免疫系统。IL-1 alpha 介导 NF-kappa-B 和三种 MAPK 途径 (p38、p42/p44、JNK) 的激活。IL-1 alpha 蛋白, Human (HEK293) 是重组蛋白, 由 159 个 (S113-A271) 氨基酸组成,在 HEK293 细胞中表达。

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规格 价格 是否有货 数量
5 μg ¥1900 In-stock
20 μg ¥4080 In-stock
100 μg ¥11000 询价
250 μg   询价  

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  • 产品资料

  • 参考文献

描述

IL-1 alpha is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1 alpha mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways[1][4]. IL-1 alpha protein, Human (HEK293) is a recombinant protein consisting of 159 amino acids (S113-A271) and is produced by HEK293 cells.

研究背景

IL-1 alpha (interleukin-1 alpha) is a major agonist of IL-1. IL-1α is present in all mesenchymal cells in particular, cells rich in IL-1α constitute tissues with a barrier function, such as keratinocytes in the skin, type 2 epithelial cells in the lung, the epithelium of the entire gastrointestinal tract, endothelial cells in blood vessels, and astrocytes in the brain[1].
The precursor of IL-1α (ProIL-1α) is processed by the Ca2+-dependent protease calpain (including caspase-1) into the mature 17 kDa form and the 16 kDa N-terminal cleavage product – the propiece of IL-1α, also termed IL-1α N-terminal peptide (IL-1NTP). The latent form of calpain is activated in cells under inflammatory conditions and especially upon loss of plasma membrane integrity, which occurs during necrosis. pro-IL-1α and mature IL-1α bind to IL-1R and induces the secretion of IL-6 and TNF[5].
IL-1α acts as an ‘alarmin’ and as a primum movens of tissue inflammation[1]. IL-1 alpha inhibits differentiation of preadipocytes and lipid accumulation[2]. IL-1 alpha induces apoptosis and inhibits the osteoblast differentiation[3]. IL-1 alpha trigger inflammation in a pathway initiated through Myd88 activation and culminated in NF-κB–induced transcription of inflammatory genes[4].

体外研究
(In Vitro)

IL-1 alpha (human) (0.1-10 ng/mL) increases the IL-1-beta mRNA levels in EC derived from adult human saphenous vein in a concentration- and time-dependent manner, and induces the release of biologically active IL-1 from endothelial cell (EC)[6].
IL-1 alpha (human) (0-1000 ng/mL; 6h) stimulates prostaglandin E2 and bone resorption in a dose-dependent manner[7].

体内研究
(In Vivo)

IL-1 alpha (10 µg/kg; i.p.) significantly increases the TG (triglyceride) level at 12 h in serum in C57BL/6J male mice[2].

生物活性

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells and the ED50 is typically 0.2-1 ng/mL. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells and the ED50 is 0.03 ug/mL.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P01583 (S113-A271)

基因 ID
中文名
重组人 GMP IL-1 alpha 蛋白(HEK293)
同用名
IL-1α; Hematopoietin-1; IL1A; IL1F1; IL-1 alpha
分子量

Approximately 22 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 8.0

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-1 alpha Protein, Human (HEK293)
目录号:
HY-P73145
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