Legumain 蛋白, Human (HEK293, His)

Legumain proteins can slowly cleave aspartyl bonds, especially under acidic conditions. Legumain Protein, Human (HEK293, His) is the recombinant human-derived Legumain protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Legumain Protein, Human (HEK293, His) is 416 a.a., with molecular weight of 52-60 kDa.

Legumain 蛋白对天冬酰胺键的水解表现出严格的特异性。此外，它还表现出缓慢裂解天冬氨酰键的能力，特别是在酸性条件下，进一步扩展了其酶的多功能性。从功能上讲，Legumain 是溶酶体/内体系统内 MHC II 类抗原呈递蛋白质加工不可或缺的一部分。最近的研究结果表明，它还通过促进 Perforin-2 (MPEG1) 的裂解和成熟，从而促进细胞质中的抗原易位，从而在交叉呈递树突状细胞的 MHC I 类抗原呈递中发挥关键作用。此外，Legumain 对于肾近曲小管中正常溶酶体蛋白降解至关重要，并且是内化 EGFR 降解所必需的，这凸显了其在细胞过程和细胞增殖调节中的重要性。

Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methylcoumarin (Z-AAN-AMC). The specific activity is 183.34 pmol/min/µg.

Human

HEK293

C-6*His

AAH03061.1 (I18-Y433)

5641  [NCBI]

IPIDDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIIHRNGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVKDLNETIHYMYKHKMYRKMVFYIEACESGSMMNHLPDNINVYATTAANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQLTEEIQRHLDARHLIEKSVRKIVSLLAASEAEVEQLLSERAPLTGHSCYPEALLHFRTHCFNWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLGHY

52-60 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.