Microtubule-associated 蛋白 tau 蛋白, Rat (P.pastoris, His)

Microtubule-associated protein tau (MAPT) critically promotes microtubule assembly and stability, influencing neuronal polarity by acting as an important junction protein. Its dual role involves C-terminal binding to axonal microtubules and N-terminal binding to neuroplasmic membrane components. Microtubule-associated protein tau Protein, Rat (P.pastoris, His) is the recombinant rat-derived Microtubule-associated protein tau protein, expressed by P. pastoris , with N-His labeled tag. The total length of Microtubule-associated protein tau Protein, Rat (P.pastoris, His) is 751 a.a., with molecular weight of ~80.4 kDa.

微管相关蛋白 tau (MAPT) 通过促进微管组装和稳定性，在细胞动力学中发挥着关键作用，可能有助于神经元极性的建立和维持。其功能结构揭示了双重作用，C 端结合轴突微管，N 端结合神经质膜成分，这意味着 tau 充当这两种结构之间的关键连接蛋白。预定的轴突极性由 tau 在神经元细胞内的定位控制，特别是在中心体定义的区域中。值得注意的是，tau 的短同工型赋予细胞骨架可塑性，而较长的同工型可能优先有助于其稳定性。MAPT 参与与各种蛋白质相互作用的复杂网络，包括 MARK1、MARK2、MARK3、SQSTM1、PSMC2、FKBP4、CSNK1D、SGK1、EPM2A、PIN1、LRRK2 和 LRP1，展示了其在细胞过程中的多方面参与。特别是，它与 LRP1 的相互作用导致内吞作用，强调了控制 tau 功能多功能性的复杂调节机制。

Rat

P. pastoris

N-His

P19332 (2A-752L)

29477  [NCBI]

AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

Approximately 80.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.