NLGN1 蛋白, Human (HEK293, C-His)

Neuroligin-1/NLGN1 Protein, a cell surface protein, actively engages in synapse function and transmission by interacting with neurexin family members. While not indispensable for initial synapse formation, NLGN1 participates in de novo presynaptic structure formation. Crucial for wakefulness and cerebral cortex synchrony, it binds to neurexins (NRXN1, NRXN2, NRXN3) and interacts with NLGN3, AIP1, PDZRN3, DLG4/PSD-95, and GOPC, collectively contributing to nervous system development. NLGN1 Protein, Human (HEK293, C-His) is the recombinant human-derived NLGN1 protein, expressed by HEK293, with C-His labeled tag. The total length of NLGN1 Protein, Human (HEK293, C-His) is 632 a.a., with molecular weight of 85-95 kDa.

Neuroligin-1 (NLGN1) 是一种细胞表面蛋白，通过与神经素家族成员的结合积极参与细胞间相互作用。它可能通过招募和聚集各种突触蛋白，在突触功能和突触信号的传递中发挥关键作用。虽然 NLGN1 可能有助于突触的初始形成，但它并不被认为是该过程不可或缺的。体外研究表明其能够启动突触前结构的从头形成，表明其参与兴奋性突触的规范。此外，NLGN1对于维持清醒质量以及清醒和睡眠期间大脑皮层活动的正常同步至关重要。该蛋白复杂的相互作用包括与神经毒素（特别是 NRXN1、NRXN2 和 NRXN3）的结合，这是由神经毒素上的硫酸乙酰肝素聚糖修饰介导的。此外，NLGN1 与 NLGN3、AIP1、PDZRN3、DLG4/PSD-95（通过 PDZ 结构域 3）和 GOPC 相互作用，共同促进其在神经系统发育中的作用。

Measured by its binding ability in a functional ELISA. When recombinant human Neuroligin-1 is immobilized at 5 μg/mL, biotinylated recombinant human Neurexin 1 beta binds with an apparent KD is 36.43 nM.

Human

HEK293

C-His

Q8N2Q7-2/NP_055747.1 (Q46-S677)

22871  [NCBI]

QKLDDVDPLVATNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGERRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARMLATKVGCNVSDTVELVECLQKKPYKELVDQDIQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGISASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQYTSTTTKVPSTDITFRPTRKNSVPVTSAFPTAKQDD

85-95 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.