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  3. OPN1MW 蛋白, Human (Cell-Free, His)

OPN1MW 蛋白, Human (Cell-Free, His)

目录号: HY-P702399
产品使用指南

OPN1MW 蛋白对视力至关重要,在视觉色素、吸收光线和促进视力方面发挥着至关重要的作用。这些色素由视蛋白组成,与顺式视黄醛形成共价键。OPN1MW 蛋白与顺式视网膜一起构成了感知光刺激所必需的复杂分子机制,从而实现了视觉过程。OPN1MW 蛋白, Human (Cell-Free, His) 是重组的 OPN1MW 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。OPN1MW 蛋白, Human (Cell-Free, His) 全长 364 个氨基酸,分子量为 46.6 kDa。

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描述

OPN1MW protein, vital for vision, plays a crucial role in visual pigments, absorbing light and facilitating vision. These pigments consist of opsin, forming a covalent bond with cis-retinal. The OPN1MW protein, along with cis-retinal, contributes to the intricate molecular mechanism essential for perceiving light stimuli, enabling the visual process. OPN1MW Protein, Human (Cell-Free, His) is the recombinant human-derived OPN1MW protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OPN1MW Protein, Human (Cell-Free, His) is 364 a.a., with molecular weight of 46.6 kDa.

研究背景

OPN1MW 蛋白是视觉过程中不可或缺的一部分,作为视觉色素的一部分发挥着至关重要的作用,视觉色素是负责吸收光和调节视觉的分子。这些色素由脱辅基蛋白(称为视蛋白)组成,它与顺式视黄醛形成共价连接。OPN1MW 蛋白和顺式视黄醛共同构成了感知光刺激所必需的复杂分子机制,从而使视觉过程得以展开。

种属

Human

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P04001 (M1-A364)

基因 ID

/

蛋白结构
N-term
10*His
OPN1MW (M1-A364)
Accession # P04001
C-term
同用名
Medium-wave-sensitive opsin 1; Green cone photoreceptor pigment; Green-sensitive opsin; GOP
氨基酸序列

MAQQWSLQRLAGRHPQDSYEDSTQSSIFTYTNSNSTRGPFEGPNYHIAPRWVYHLTSVWMIFVVIASVFTNGLVLAATMKFKKLRHPLNWILVNLAVADLAETVIASTISVVNQVYGYFVLGHPMCVLEGYTVSLCGITGLWSLAIISWERWMVVCKPFGNVRFDAKLAIVGIAFSWIWAAVWTAPPIFGWSRYWPHGLKTSCGPDVFSGSSYPGVQSYMIVLMVTCCITPLSIIVLCYLQVWLAIRAVAKQQKESESTQKAEKEVTRMVVVMVLAFCFCWGPYAFFACFAAANPGYPFHPLMAALPAFFAKSATIYNPVIYVFMNRQFRNCILQLFGKKVDDGSELSSASKTEVSSVSSVSPA

分子量

46.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

OPN1MW Protein, Human (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
OPN1MW Protein, Human (Cell-Free, His)
目录号:
HY-P702399
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