TGM2/Transglutaminase 2 蛋白, Human (sf9, His)

In summary, TGM2 is a multifunctional enzyme that plays crucial roles in various cellular processes. It acts as a protein-glutamine gamma-glutamyl transferase, mediating the cross-linking of proteins involved in cellular functions. TGM2/Transglutaminase 2 Protein, Human (sf9, His) is the recombinant human-derived TGM2/Transglutaminase 2 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of TGM2/Transglutaminase 2 Protein, Human (sf9, His) is 687 a.a., with molecular weight of ~80 kDa.

转谷氨酰胺酶 2 (TGM2) 是一种钙依赖性酰基转移酶，可促进肽结合的谷氨酰胺和各种伯胺之间形成共价键，从而导致蛋白质交联或胺化。它在多种生物过程中发挥着至关重要的作用，包括骨骼发育、血管生成、伤口愈合、细胞分化、染色质修饰和细胞凋亡。TGM2 通过介导参与重要细胞功能的各种蛋白质的交联，充当蛋白质-谷氨酰胺 γ-谷氨酰转移酶。在生理条件下，其蛋白质交联活性被GTP抑制，并在应激过程中缓解对Ca(2+)反应的抑制。当分泌时，TGM2 催化细胞外基质蛋白的交联，形成稳定的支架。在细胞凋亡过程中，TGM2 通过促进细胞骨架蛋白的交联来促进细胞质的浓缩，并介导支架的不可逆形成，从而在吞噬作用清除之前稳定垂死的细胞，从而防止有害的细胞内成分泄漏。此外，TGM2 执行多种蛋白质翻译后修饰，包括将血清素、多巴胺、去甲肾上腺素或组胺氨化为谷氨酰胺残基，分别产生蛋白质血清素化、多巴胺化、去甲肾上腺素化或组胺化。它通过介导组蛋白 H3 的血清素化和多巴胺化在染色质组织中发挥关键作用。此外，TGM2 在腹侧被盖区 (VTA) 神经元中充当核多巴胺神经传递独立作用的介导者，调节奖励系统中与复发相关的转录可塑性。它还通过介导血清素化和随后的 ATP2A2/SERCA2 失活来调节静脉重塑。此外，TGM2 作为蛋白质脱酰胺酶，将特定的谷氨酰胺残基转化为谷氨酸，并且可以作为异肽酶裂解先前形成的交联。它还可以独立于其酰基转移酶活性参与信号转导通路，在 α-1 肾上腺素受体介导的磷脂酶 C-δ (PLCD) 活性刺激中充当信号转导器，并且是将 α-1 肾上腺素激动剂与刺激偶联所必需的磷酸肌醇脂质代谢的影响。TGM2 具有细胞毒活性，可独立于其酰基转移酶活性诱导细胞凋亡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

P21980-1 (M1-A687)

7052  [NCBI]

Approximately 80 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 2 mM DTT, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.