Tucotuzumab  (Synonyms: 西莫白介素单抗)

Tucotuzumab (Anti-EPCAM Recombinant Antibody) is an antigen-specific IgG1 monoclonal antibody that targets human epithelial cell adhesion molecule (EpCAM). Tucotuzumab links two IL-2 molecules and is an immunosuppressant and anti-tumor active molecule^[1].

Human IgG1

Human IgG1 kappa, Isotype Control

Human

EpCAM/TROP1/CD326

Tucotuzumab 靶向肿瘤并通过 IL-2 应答性免疫细胞刺激抗肿瘤免疫反应，以及通过其 Fc 受体刺激免疫细胞^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Tucotuzumab (75 μg; 瘤内注射; 每日 5 次，每次15 μg (总剂量 75 μg)，各用50 μL PBS; 在第 10 天和第 14 天)，肿瘤生长抑制显著增强，生存率提高^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

4072  [NCBI]

P16422

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

144.34 kDa

液体

Colorless to light yellow

西莫白介素单抗

Shipping with dry ice.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1X10⁶ MCF-7 cells with Tucotuzumab (HY-P99328, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Loaded EpCAM/TROP1 Protein, Human (Biotinylated, HEK293, His-Avi, HY-P72367) on HIS1K biosensor, can bind Tucotuzumab with an affinity constant of 3.827E-11 M as determined in BLI assay.

• [1]. Erik E Johnson, et al. Radiofrequency ablation combined with KS-IL2 immunocytokine (EMD 273066) results in an enhanced antitumor effect against murine colon adenocarcinoma. Clin Cancer Res. 2009 Aug 1;15(15):4875-84.  [Content Brief]