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  4. Doxorubicin

Doxorubicin  (Synonyms: 阿霉素; Hydroxydaunorubicin)

目录号: HY-15142A
产品使用指南

Doxorubicin (Hydroxydaunorubicin) 是一种有细胞毒性的蒽环类广谱抗生素,常用作肿瘤化疗剂。Doxorubicin 具有荧光性。Doxorubicin 抑制拓扑异构酶 II (topoisomerase-II),IC50 为 2.67 μM,从而抑制 DNA 复制。Doxorubicin 下调 AMPK 的基础磷酸化以及下游 acetyl-CoA 羧化酶。Doxorubicin 诱导凋亡 (apoptosis) 和自噬 (autophagy)。Doxorubicin 抑制人 DNA 拓扑异构酶 I (topoisomerase I),IC50 为 0.8 μM。

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Doxorubicin Chemical Structure

Doxorubicin Chemical Structure

CAS No. : 23214-92-8

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Doxorubicin 的其他形式现货产品:

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 353 篇科研文献

WB
IF
IHC

    Doxorubicin purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Nov 25;10(12):887.  [Abstract]

    Doxorubicin or CPT-11 significantly promoted KRT8 expression in chordoma cells in vitro. Representative images of immunofluorescence staining of KRT8 of CM318 and UCH1 cell line.

    Doxorubicin purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Nov 25;10(12):887.  [Abstract]

    Doxorubicin or CPT-11 significantly promoted KRT8 expression in chordoma cells in vitro. Western blotting analysis and quantification of KRT8 protein expression (normalized to GAPDH expression).

    Doxorubicin purchased from MCE. Usage Cited in: Free Radic Biol Med. 2019 Jan;130:557-567.  [Abstract]

    Representative pictures of Live/Dead staining of effects of 7,8-DHF on high dose of Dox-induced cell death and MMP reduction of H9c2 cells.

    Doxorubicin purchased from MCE. Usage Cited in: J Cell Mol Med. 2019 Sep;23(9):6034-6047.  [Abstract]

    Effects of YWPC (50 mg/L for 24 h) on DOX (5 μM for 24 h)-induces dissipation of mitochondrial membrane potential measured in H9C2 cells loaded with JC-1 using fluorescence microscopy.

    Doxorubicin purchased from MCE. Usage Cited in: Nat Commun. 2018 Oct 8;9(1):4139.  [Abstract]

    One hour after treated with UV or 2 h after treated with MMS, Camptothecin (CPT) (10 μM), or Doxorubicin (DOX; 0.5 μM), HEK293T cells expressing HA-SUMO2 and His-RhoB are subjected to sumoylation assay to detect the SUMO2 conjugation of RhoB.

    Doxorubicin purchased from MCE. Usage Cited in: J Clin Invest. 2018 Jan 2;128(1):483-499.  [Abstract]

    Immunoblotting for EZH2 in lysates of immortalized mouse podocytes after treatment with Adriamycin (300 ng/mL) for 48 hours (n=6).

    Doxorubicin purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2018 Sep 19;37(1):232.  [Abstract]

    Immunoblotting shows that both P-gp protein expression is significantly decreased in 24 h after US exposure in MCF-7/ADR and HEPG2/ADM cells.

    Doxorubicin purchased from MCE. Usage Cited in: Nanoscale Res Lett. 2018 Nov 3;13(1):350.  [Abstract]

    HY-15142

    Doxorubicin purchased from MCE. Usage Cited in: Cell Signal. 2017 May 1;36:108-116.  [Abstract]

    PAQR3 affects DNA damage repair. AGS cells are treated with different doses of VP-16 (for 24 h), CDDP (for 24 h) and Doxorubicin (for 10 h) as indicated, followed by immunoblotting with the antibodies.

    Doxorubicin purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;42(3):965-973.  [Abstract]

    U937 cells are treated with Thapsigargin (TG, 1 μM), NSC 125973 (PTX, 5nM) as well as Doxorubincin (DOX, 1 μM) for 12 hours and the indicated proteins are detected by Western blot.

    Doxorubicin purchased from MCE. Usage Cited in: Nat Med. 2016 May;22(5):547-56.  [Abstract]

    Immunofluorescent staining for α-actinin (ACTN2) and cardiac troponin T (TNNT2) to demonstrate sarcomeric organization in hiPSC–CMs derived from patients who do not experience Doxorubicin–induced cardiotoxicity (DOX) versus those who do experience Doxorubicin-induced cardiotoxicity (DOXTOX) after 24 h treatment with Doxorubicin. Scale bar, 20 µm.
    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Doxorubicin (Hydroxydaunorubicin), a broad-spectrum anthracycline antibiotic with cytotoxic properties, is an anti-cancer chemotherapy agent. Doxorubicin has fluorescence properties. Doxorubicin inhibits topoisomerase II with an IC50 of 2.67 μM, thus stopping DNA replication. Doxorubicin reduces basal phosphorylation of AMPK and its downstream target acetyl-CoA carboxylase. Doxorubicin induces apoptosis and autophagy[1][2]. Doxorubicin inhibits human DNA topoisomerase I with an IC50 of 0.8 μM[3].

    IC50 & Target[1][2][3][7]

    Topoisomerase I

    0.8 μM (IC50)

    Topoisomerase II

    2.67 μM (IC50)

    Daunorubicins/Doxorubicins

     

    HIV-1

     

    体外研究
    (In Vitro)

    Doxorubicin (Hydroxydaunorubicin) 和辛伐他汀 (HY-17502) 在最高测试浓度 (分别为2 μM和10 μM) 联合使用可杀死 97% 的 Hela 细胞[4]
    Doxorubicin 可以标记神经元细胞,且罗丹明滤袋下呈鲜红色,在儿茶酚胺滤袋下呈淡红橙色[8]
    Doxorubicin (5 μM; 10-30 分钟) 在 B16-F10 黑色素瘤细胞系 CRL-6475 细胞中能以时间依赖方式在细胞中积累,且可以通过绿色或红色荧光来进行检测 (绿色荧光的检测灵敏度更高), 最大激发波长 (λex) 和最大发射波长为 (λem) 分别为 470 nm 和 560 nm[10].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    Doxorubicin (4-8 mg/kg) 在PC3 异种抑移植裸鼠中会延迟肿瘤的生长,降低小鼠中 c-FLIP 水平的表达[5]
    Doxorubicin (腹腔注射; 单剂量 (10 mg/kg) /一天一次连续 10 天 (1 mg/kg) /一周一次连续5周 (2 mg/kg)) 在 Sprague-Dawley 大鼠中具有心脏毒性,但是与一次性给药 10 mg/kg 相比,通过多次在几天或几周累计给药 10 mg/kg 的方式可以提高大鼠的存活率[6]
    Doxorubicin (4%- 20%; Intrastriatal 注射; 单剂量) 在 Sprague-Dawley 大鼠中具有神经毒性[8]
    Doxorubicin 在酸性条件下可以通过 PH-敏感键偶联在金纳米颗粒 (Au NPs) 上面,从而能通过血脑屏障,最大的吸收波长为528 nm[9]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Athymic male nude mice model of xenografts of PC3 prostate carcinoma cells[5]
    Dosage: 2 mg/kg, 4 mg/kg, 8 mg/kg
    Administration: ntraperitoneal injection (i.p.); Single dose .After injected PC3 cells (4 × 106) subcutaneously into the flank of mice.
    Result: A dose of 2 mg/ kg did not affect tumor growth while higher dosages (4 mg/kg, 8 mg/kg) delayed tumor growth initially.
    Animal Model: Male Sprague-Dawley rats model[6]
    Dosage: 10 mg/kg (schedule 1), 1 mg/kg (schedule 2), 2 mg/kg (schedule 3)
    Administration: Intraperitoneal injection (i.p.) ; Single dose (schedule 1). Intraperitoneal injection (i.p.); Once daily for 10 days (schedule 2). Intraperitoneal injection (i.p.); Once per week, for 5 weeks(schedule 3).
    Result: In schedule 1, caused 30% of the rats to die at the end of week 2 and 80% by day 28.
    In schedule 2 , caused 55% of the rats to die at the end of week 13 and 80% by day 107.
    In schedule 3, caused 42% of the rats to die at the end of week 13 and 80% by day 98.
    Animal Model: Male Sprague-Dawley rats[8]
    Dosage: 1%, 2%, 4%, 5%, 6%, 10%, 20%
    Administration: Intrastriatal injection; Single dose
    Result: In doses of 4, 5, 6, 10 or 20% caused obvious loss of ipsilateral SNc and VTA neuronsz and doses of 1 or 2% failed to produce obvious neuron loss.
    Clinical Trial
    分子量

    543.52

    Formula

    C27H29NO11

    CAS 号
    中文名称

    阿霉素;多柔比星

    结构分类
    初始来源

    Streptomyces peucetius.

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.

    溶解性数据
    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案源自文献,仅供参考,建议您先取少量样品进行尝试。

    • 方案 一

      Doxorubicin (dissolved with distilled water) is incorporated into 0.035% CaCl2 solution[5].

    • 方案 二

      Doxorubicin (Adriamycin) is prepared in vehicle (PBS)[6].

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料
    参考文献
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    目录号:
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