1. Cell Cycle/DNA Damage Stem Cell/Wnt Cytoskeleton TGF-beta/Smad
  2. Organoid ROCK
  3. Y-27632 dihydrochloride

Y-27632 dihydrochloride 

目录号: HY-10583 纯度: 99.94%
COA 产品使用指南

Y-27632 dihydrochloride 是一种具有口服活性的、ATP 竞争性的 ROCK (Rho-kinase) 抑制剂 (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM)。Y-27632 dihydrochloride 表现出抗癫痫的作用。

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Y-27632 dihydrochloride Chemical Structure

Y-27632 dihydrochloride Chemical Structure

CAS No. : 129830-38-2

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥770
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2 mg ¥450
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5 mg ¥700
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10 mg ¥900
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50 mg ¥3200
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100 mg ¥5800
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200 mg ¥8800
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Other Forms of Y-27632 dihydrochloride:

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 228 篇科研文献

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IF

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2018 Jun 28;37(1):128.  [Abstract]

    MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Oncotarget. 2017 Jul 31;8(47):82506-82530.  [Abstract]

    SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) cells were either untreated (-) or treated (+) with 10mM Y27632 over a period of 48 hr. SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) clones were either untreated (-) or treated (+) with 10 mM Y27632 followed by stimulation without (-) or with (+) 5 ng/mL of TGFβ1 for 48 hr.

    查看 ROCK 亚型特异性产品:

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects[1][2][3][4].

    IC50 & Target[1]

    ROCK-I

    220 nM (Ki)

    ROCK-II

    300 nM (Ki)

    PKN

    3.1 μM (Ki)

    Citron kinase

    5.3 μM (Ki)

    PKCα

    73 μM (Ki)

    PKA

    25 μM (Ki)

    体外研究
    (In Vitro)

    Y-27632 dihydrochloride (1-5 μM;0-60 分钟) 促进脂肪组织来源的干细胞 (ADSCs) 的神经元分化[3]
    Y-27632 dihydrochloride (1-5 μM;0-60 分钟) 诱导 ADSCs 中 NSE、MAP-2 和巢蛋白的表达[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[3]

    Cell Line: Adipose tissue-derived stem cells (ADSCs)
    Concentration: 20 μM
    Incubation Time: 24 hours
    Result: Resulted in the up-regulation of NSE, MAP-2 and nestin protein levels by 25.3, 3.1 and 2.5 fold, respectively, compared to control cells not treated by Y-27632.
    体内研究
    (In Vivo)

    Y-27632 dihydrochloride (口服灌胃;30 mg/kg;每日一次;4 周) 预防二甲基亚硝胺诱导的大鼠肝纤维化[1]
    Y-27632 dihydrochloride (口服灌胃;5- 10 mg/kg;once) 对 PTZ 和 MES 诱发的癫痫有抗癫痫作用[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats injected with dimethylnitrosamine[1]
    Dosage: 30 mg/kg
    Administration: Oral gavage; 30 mg/kg; once daily; 4 weeks
    Result: Decreased the occurrence of dimethylnitrosamine-induced hepatic fibrosis and reduced the collagen and hydroxyproline content and α-smooth muscle actin expression in the liver.
    Animal Model: Male Swiss albino mice injected with PTZ (pentylenetetrazole) or induced by MES (maximal electroconvulsive shock)[2]
    Dosage: 5-10 mg/kg
    Administration: Oral gavage; 5-10 mg/kg; once
    Result: Prolonged the onset time of myoclonic jerks when compared with those observed in the saline group (P<0.05).
    Prolonged the onset time of clonic convulsions when compared with saline group (P<0.05).
    Prevented the occurrence of tonic hindlimb extensions and death.
    分子量

    320.26

    Formula

    C14H23Cl2N3O

    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : 100 mg/mL (312.25 mM; 超声助溶)

    DMSO 中的溶解度 : 33.33 mg/mL (104.07 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.1225 mL 15.6123 mL 31.2246 mL
    5 mM 0.6245 mL 3.1225 mL 6.2449 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.81 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.81 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

      20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 220 mg/mL (686.94 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.98%

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.1225 mL 15.6123 mL 31.2246 mL 78.0616 mL
    5 mM 0.6245 mL 3.1225 mL 6.2449 mL 15.6123 mL
    10 mM 0.3122 mL 1.5612 mL 3.1225 mL 7.8062 mL
    15 mM 0.2082 mL 1.0408 mL 2.0816 mL 5.2041 mL
    20 mM 0.1561 mL 0.7806 mL 1.5612 mL 3.9031 mL
    25 mM 0.1249 mL 0.6245 mL 1.2490 mL 3.1225 mL
    30 mM 0.1041 mL 0.5204 mL 1.0408 mL 2.6021 mL
    40 mM 0.0781 mL 0.3903 mL 0.7806 mL 1.9515 mL
    50 mM 0.0624 mL 0.3122 mL 0.6245 mL 1.5612 mL
    60 mM 0.0520 mL 0.2602 mL 0.5204 mL 1.3010 mL
    80 mM 0.0390 mL 0.1952 mL 0.3903 mL 0.9758 mL
    100 mM 0.0312 mL 0.1561 mL 0.3122 mL 0.7806 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Y-27632 dihydrochloride
    目录号:
    HY-10583
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