2. Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR MAPK/ERK Pathway Stem Cell/Wnt Metabolic Enzyme/Protease
  3. Src Akt ERK TAM Receptor c-Met/HGFR MMP HIF/HIF Prolyl-Hydroxylase VEGFR
  4. AG01

AG01 是一种抗颗粒蛋白前体 (GP88) 单克隆抗体。AG01 抑制三阴性乳腺癌 (TNBC) 细胞增殖和迁移，降低磷酸化蛋白激酶 p-Src、p-AKT 和 p-ERK 的表达，降低 Axl、c-MET、HIF-1α 和 VEGF 等致癌蛋白的表达。AG01 在 TNBC 异种移植小鼠模型中抑制了肿瘤生长和体内 Ki67 的表达。AG01 可用于 TNBC 等癌症的研究。

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AG01

AG01 Chemical Structure

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AG01 相关抗体

Top Publications Citing Use of Products

查看 Src 亚型特异性产品:

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Lck Fyn Blk Hck Lyn Yes SRC-3

查看 Akt 亚型特异性产品:

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Akt Akt1 Akt2 Akt3

查看 ERK 亚型特异性产品:

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ERK ERK1 ERK2 ERK5 ERK8

查看 TAM Receptor 亚型特异性产品:

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Axl Mer Tyro3

查看 MMP 亚型特异性产品:

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

查看 HIF/HIF Prolyl-Hydroxylase 亚型特异性产品:

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HIF-1α HIF

查看 VEGFR 亚型特异性产品:

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VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

AG01 is a monoclonal antibody against progranulin (GP88). AG01 inhibits triple-negative breast cancer (TNBC) cell proliferation and migration, reduces the expression of phosphorylated protein kinases p-Src, p-AKT, and p-ERK, and reduces the expression of oncogenic proteins such as Axl, c-MET, HIF-1α, and VEGF. AG01 inhibits tumor growth and Ki67 expression in a TNBC xenograft mouse model. AG01 can be used in the research of TNBC and other cancers[1].

反应种属

Human
IC50 & Target[1]

ERK1

 

ERK2

 

MMP-2

 

Axl

 

HIF-1α

 

体外研究
(In Vitro)

AG01 (0-300 µg/mL，48-72 小时) 可抑制 MDA-MB-231 和 HS578-T 细胞增殖，降低 Ki67 阳性细胞核数量[1]。
AG01 (0-100 µg/mL，5 小时) 可抑制 MDA-MB-231 和 HS578-T 细胞迁移和侵袭[1]。
AG01 (100 µg/mL，24-72 小时) 可降低 MDA-MB-231 和 HS578-T 细胞中 AKt、Src、Erk1/2 的磷酸化状态[1]。
AG01 (100 µg/mL，24-72 小时) 在 MDA-MB-231、HS578-T 细胞中可降低 Axl、c-MET、ICAM-1、MMP-2、SNAIL、HIF-1α、VEGF、内源性前颗粒蛋白、SPARC、细胞粘附和迁移蛋白-纤连蛋白的表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

AG01 相关抗体:

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 0, 25, 50, 100, 200, 300 µg/mL
Incubation Time: 48, 72 h
Result: Inhibited cells proliferation, resulted in a dose-dependent inhibition of MDA-MB-231 and HS578-T cells proliferation by 44% and 51% at 300 µg/mL compared with control.

Cell Migration Assay [1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 25, 50, 100 µg/mL
Incubation Time: 5 h
Result: Inhibited cells migration in a dose-dependent, with the highest reduction of 78.2%, 77.5% at 100 µg/mL for MDA-MB-231, HS578-T cells.

Western Blot Analysis[1]

Cell Line: MDA-MB-231, HS578-T cells
Concentration: 100 µg/mL
Incubation Time: 24, 48, 72 h
Result: Decreased the phosphorylation status of Akt (14.8%), Src (23.6%), and Erk1/2 (20.3%) at 24 h and decreased phosphorylation of Akt (63%), Src (55.4%), and Erk1/2 (55.2%) at 72 h compared to control, decreased the ratio of phosphorylated protein to its total corresponding protein expression for Akt (71.9%), Src (49.8%), and Erk1/2 (62%) at 72 h in MDA-MB-231 cells.
Decreased the phosphorylation status of Akt (44.5%), Src (31.5%), and ERK1/2 (35.5%) at 24 h while decreased FAK phosphorylation (44.5%) at 48 h, decreased the ratio of phosphorylated protein to its total corresponding protein expression for p-Src/Src (44.3%), p-Akt/Akt (47.3%), and p-ERK1/2/ERK (46%) at 48 h in HS578-T cells.
Reduced c-MET expression of 59.7%.
Reduced SNAIL expression of 23.3% and 89%, ICAM-1 of 93.8% and 33.8%, AXL of 13.2% and 33.4% in MDA-MB-231 and HS578-T cells, respectively, reduced endogenous progranulin level in time-dependent reduction with MDA-MB-231 showing 37.7% reduction by 72 h and HS578-T showing almost a 100% reduction by 48 h.
Reduced cell adhesion and migratory protein-fbronectin in time-dependent decrease of 25.4% at 72 h as compared to its control lysate in MDA-MB-231 cells.
体内研究
(In Vivo)

AG01 (10 mg/kg，腹腔注射，每周两次，共 40 天) 可抑制 MDA-MB-231 异种移植裸鼠模型中的肿瘤生长和 Ki67 表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MDA-MB-231 (1.5x106) xenograft nude mice (6-8-week-old, female) model[1]
Dosage: 10 mg/kg
Administration: i.p., twice weekly, 40 days
Result: Reduced Vt/V0 ratio and tumor weights by 50% compared to control human IgG treated group, reduced Ki67 expression in tumor sections by 52.5% compared to control groups, showed a 50% reduction of the mitotic index compared to control and 65% reduction of relative microvessel numbers compared to control HuIgG-treated animals.
基因 ID

2896  [NCBI]

Accession

NP_002078.1
靶点

PGRN/GP88
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

AG01 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AG01AG 01AG-01SrcAktERKTAM Receptorc-Met/HGFRMMPHIF/HIF Prolyl-HydroxylaseVEGFRPKBProtein kinase BExtracellular signal regulated kinasesTyro3AxlMerMatrix metalloproteinasesHypoxia-inducible factorsHIFsHIF-PHVascular endothelial growth factor receptorAntibodyMDA-MB-231 cellsHS578-T cellsTNBCnude miceInhibitorinhibitorinhibit

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