2. Cell Cycle/DNA Damage Autophagy Anti-infection NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Topoisomerase Autophagy Bacterial Reactive Oxygen Species Endogenous Metabolite Antibiotic Parasite
  4. Berberine

Berberine  (Synonyms: 黄连素; Natural Yellow 18)

目录号: HY-N0716
产品使用指南

摩尔计算器

Berberine (Natural Yellow 18) 是从中草药黄连中分离出来的一种生物碱，常用作抗生素。Berberine (Natural Yellow 18) 诱导活性氧 (ROS) 生成并抑制 DNA 拓扑异构酶 (topoisomerase)。Berberine (Natural Yellow 18) 具有抗肿瘤特性。硫酸盐形式 (HY-N0716B) 可提高生物利用度。

在相同的摩尔浓度下，化合物盐形式与游离形式有相同的生物活性，但盐形式 Berberine sulfate 通常具有更好的水溶性和稳定性。

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Berberine Chemical Structure

Berberine Chemical Structure

CAS No. : 2086-83-1

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Other Forms of Berberine:

Berberine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 35 篇科研文献

WB
Proliferation Assay
Cell Viability Assay

    Berberine purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5 µM; 10 days) significantly inhibits NCI-H441 colony formation.

    Berberine purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 2.5, 5, 10 µM; 48 h) inhibits EGFR phosphorylation in a dose-dependent matter in A431cells.

    Berberine purchased from MCE. Usage Cited in: BMC Pharmacol Toxicol. 2023 May 11;24(1):29.  [Abstract]

    Berberine (BBR; 0.625, 1.25, 2.5, 5, 10 µM; 48 h) inhibits the viability of A431cells in a dose-dependent matter.

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Berberine (Natural Yellow 18) is an alkaloid isolated from the Chinese herbal medicine Huanglian, as an antibiotic. Berberine (Natural Yellow 18) induces reactive oxygen species (ROS) generation and inhibits DNA topoisomerase. Berberine (Natural Yellow 18) has antineoplastic properties. The sulfate form (HY-N0716B) improves bioavailability[1].

    IC50 & Target

    ROS[1]
    DNA topoisomerase[1]
    体外研究
    (In Vitro)

    Berberine (1.25-160 μM; 72 hours) has potential inhibitory effects on the proliferation of four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29[1].
    Berberine (1.25-160 μM; 24-72 hours) induces a time- and dose-dependent inhibition of LoVo cell growth[1].
    LoVo cells are exposure to Berberine (10-80 μM) for 24 h. Cell cycle analysis of 40 μM Berberine-treated LoVo cells by flow cytometry shows accumulation of cells in the G2/M phase[1].
    Berberine (10-80 μM) suppresses cyclin B1, cdc2 and cdc25c protein expression after 24 h, especially at the dose of 80.0 μM[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Berberine 相关抗体:

    Cell Proliferation Assay[1]

    Cell Line: Four colorectal carcinoma cell lines LoVo, HCT116, SW480, and HT-29
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 72 hours
    Result: Inhibited the proliferation of four cell lines. The IC50 ranged from 40.8±4.1 μM (LoVo) to 98.6±2.9 μM (HCT116).

    Cell Proliferation Assay[1]

    Cell Line: Colorectal carcinoma cell lines LoVo
    Concentration: 1.25, 2.5, 5, 10, 20, 40, 80, and 160 μM
    Incubation Time: 24, 48, 72 hours
    Result: Induced a time- and dose-dependent inhibition of cell growth. By 72 h, 160.0 μM induced 71.1±1.9 % growth inhibitions in LoVo cells.

    Cell Cycle Analysis[1]

    Cell Line: LoVo cells
    Concentration: 0, 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Exposure to 40.0 μM induced G2/M-phase cell cycle arrest, an increase in the G2/M-phase population and a progressive decline in the G1 population.

    Western Blot Analysis[1]

    Cell Line: LoVo cells
    Concentration: 10, 20, 40, or 80 μM
    Incubation Time: 24 hours
    Result: Suppressed cyclin B1, cdc2 and cdc25c protein expression.
    体内研究
    (In Vivo)

    Berberine (10, 30, or 50 mg/kg/day; gastrointestinal gavage; for 10 consecutive days) inhibits the growth of human colorectal adenocarcinoma in vivo. Berberine at doses of 30 and 50 mg/kg/day taken by gastrointestinal gavage shows inhibitory rates of 33.1% and 45.3% on the human colorectal adenocarcinoma xenograft growth in nude mice[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 5-week-old BALB/c nu/nu mice with human colorectal adenocarcinoma LoVo xenografts[1]
    Dosage: 10, 30, or 50 mg/kg/day
    Administration: Gastrointestinal gavage; for 10 consecutive days
    Result: Showed inhibitory rates of 33.1 % and 45.3 % at doses of 30 and 50 mg/kg/day.
    Clinical Trial
    分子量

    336.36

    Formula

    C20H18NO4+
    CAS 号
    中文名称

    黄连素；小檗碱
    结构分类
    初始来源
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    纯度 & 产品资料
    参考文献

    Berberine 相关分类

    • 摩尔计算器

    • 稀释计算器

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Berberine2086-83-1Natural Yellow 18黄连素小檗碱Natural Yellow18Natural Yellow-18TopoisomeraseAutophagyBacterialReactive Oxygen SpeciesEndogenous MetaboliteAntibioticParasiteInhibitorinhibitorinhibit

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