2. Apoptosis
  3. Caspase Apoptosis
  4. IGN523

IGN523 是一种抗 CD98 抗体 (hCD98，KD = 0.55 nM)。IGN523 诱导抗体依赖的细胞介导的细胞毒作用 (ADCC) 活性，溶酶体膜透化并抑制必需氨基酸转运功能，最终导致 caspase-3 和 caspase-7 介导的肿瘤细胞凋亡 (apoptosis)。IGN523 在多种肿瘤异种移植模型中抑制肿瘤生长。IGN523 可用于非小细胞肺癌 (NSCLC)，急性髓系白血病 (AML) 等癌症的研究。

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IGN523

IGN523 Chemical Structure

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IGN523 相关抗体

Top Publications Citing Use of Products

查看 Caspase 亚型特异性产品:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

IGN523 is an anti-CD98 antibody (hCD98, KD = 0.55 nM). IGN523 induces antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lysosomal membrane permeabilization, and inhibition of essential amino acid transport, ultimately leading to caspase-3 and caspase-7-mediated apoptosis of tumor cells. IGN523 inhibits tumor growth in multiple tumor xenograft models. IGN523 is useful in the research of non-small cell lung cancer (NSCLC), acute myeloid leukemia (AML), and other cancers.[1].

IC50 & Target[1]

Caspase 3

 

Caspase-7

 

体外研究
(In Vitro)

IGN523 在 Ramos、KG-1 和 OCI-AML-3 细胞中诱导出强烈的 ADCC 活性，EC50 分别为 18.6 ng/mL、73.8 ng/mL 和 83.3 ng/mL，且不会降低 IGN523 细胞中的 ADCC 活性[1]。
IGN523 (2 µg/mL，30 分钟/48 小时) 在 Ramos、KG-1 和 OCI-AML-3 细胞中可诱导 caspase 依赖性细胞凋亡，增加溶酶体膜通透性，并降低氨基酸转运[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IGN523 相关抗体:

Apoptosis Analysis[1]

Cell Line: Ramos, KG-1 and OCI-AML-3 cells
Concentration: 2 µg/mL
Incubation Time: 48 h
Result: Decreased cell viability in the presence of AbXL (10 µg/mL) (Ramos: 70% reduction; OCI-AML-3: 76% reduction; KG-1: 80% reduction) , but not when used alone.
Increased caspase-3 and caspase-7 activity (Ramos: 3.3-fold; OCI-AML-3: 7.5-fold and KG-1: 6.6-fold).

Immunofluorescence[1]

Cell Line: Ramos cells
Concentration: 2 µg/mL
Incubation Time: 4℃ for 30 min
Result: Showed the CD98-IGN523 complex localized to lysosome-associated membrane protein LAMP-1 structures, increased the rate of internalization, capped CD98 at the cell surface.
体内研究
(In Vivo)

IGN523 (15 mg/kg，腹腔注射，每周一次，两次/三次) 可抑制 Ramos 和 Dau 异种移植小鼠模型中的肿瘤生长[1]。
IGN523 (15 mg/kg，腹腔注射，每周一次，两次/三次/四次) 可抑制 AML 和原发性 NSCLC 异种移植小鼠模型中的肿瘤生长[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Ramos and Dau xenografts mice model[1]
Dosage: 15 mg/kg
Administration: i.p., once a week, twice/three times
Result: Inhibited tumor growth, with TGIs of 98%, 76 %, respectively.
Animal Model: AML (HL-60, OCI-AML-3 (NOD/SCID) and KG-1 (1 x 107) and NSCLC (IGN-LNG-12 and IGN-LNG-54) (1 x 106) xenografts NOD/SCID or CB.17-SCID mice model[1]
Dosage: 15 mg/kg
Administration: i.p., once a week, twice/three times/four times
Result: Reduced tumor growth compared with isotype control in all 5 xenograft models, with TGIs of HL-60 (94%), OCI-AML-3 (74%), KG-1 (53%), IGN-LNG-12 (44%) and IGN-LNG-54 (56%), without inducing body weight loss. Completely eliminated CD98 expression and CD98+-tumor cells.
基因 ID

6520  [NCBI]

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

IGN523 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IGN523IGN 523IGN-523CaspaseApoptosisAntibodyapoptosisNSCLCAMLRamos cellsKG-1 cellsOCI-AML-3 cellsNOD/SCID or CB.17-SCID miceInhibitorinhibitorinhibit

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