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  5. G418 Selective Antibiotic, Sterile

G418 Selective Antibiotic, Sterile 

G418 选择性抗生素无菌溶液

Cat. No.: HY-K1056
Manual SDS

MCE G418 Selective Antibiotic, Sterile 是一种氨基糖苷类抗生素,主要通过影响 80S 核糖体的功能来干扰蛋白质的合成,对细菌、酵母、高等植物、哺乳动物细胞等均有毒性。

G418 Selective Antibiotic, Sterile

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample (1 mL)   Apply now  
10 mL ¥402 In-stock
50 mL ¥1450 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

G418 selective antibiotic is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

Resistance to G418 selective antibiotic is conferred by the E. coli APH (3) I and APH (3) II resistance genes. The gene becomes APH (3) II on Tn5 and APH (3) I on Tn601. Therefore, G-418 selective antibiotic can be used to select and maintain prokaryotic or eukaryotic cells carrying the G418 resistance gene.

Publications
Storage

-20°C; 2 years.

Protocol

1.   The working concentration of G418 selective antibiotic varies with cell type, media, growth conditions and cell metabolic rate.

选择性抗生素
Cell Transposon Gene Concentration (mg/L) Remark
Slime mold Tn5 APH (3) II
10
35
Cells grow in culture
Cells attached to
freeze-dried bacteria
Mammalian Tn5 APH (3) II
400
200
Used to filter
Used to maintain
Plant Tn5 APH (3) II 10 Used to filter
Yeast Tn601 APH (3) I
500
125
Used to filter
Used to maintain

1.   Before stable transfected cell lines can be selected, the optimal G418 selective antibiotic concentration needs to be determined by performing a kill curve titration.

   1)    Seed the parental cell line in a suitable culture plate at a cell density of 20-25% and incubate the cells for 24 hours at 37°C.

   2)   Remove medium and then add medium with various concentrations of G418 selective antibiotic (such as 0, 50, 100, 200, 400, 800, and 1000 μg/mL) and incubate at 37°C.

   3)   Refresh the selective medium every 2-3 days and observe the percentage of surviving cells over time.

   4)   Determine the lowest concentration of antibiotic that kills a large majority of the cells within 14 days. This concentration should be used for selection of a stable transfected cell line.

Components
Components HY-K1056-10 mL HY-K1056-50 mL
G418 Selective Antibiotic, Sterile (50 mg/mL) 10 mL 50 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
G418 选择性抗生素无菌溶液
目录号:
HY-K1056
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