LPd peroxida probe  (Synonyms: 脂质过氧化绿色探针)

LPd peroxida probe, a marker of ferroptosis, is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. LPd peroxida probe reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 玻璃底培养皿中的细胞在 RPMI 1640 中用 50 µM H₂O₂ 在 37 °C 下处理 2 小时。去除 H₂O₂ 后，细胞用汉克氏平衡盐溶液 (HBSS) 洗涤 3 次。
2. 在 37°C 下用 10 µM LPd peroxida probe 在 HBSS 中处理细胞 30 分钟。去除 LPd peroxida probe 后，细胞用 HBSS 洗涤 3 次。
3. 使用 BZ-8000 荧光显微镜从 3 个独立的培养皿中获取荧光图像^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

840.85

C₅₁H₄₁N₂O₈P

1448846-35-2

固体

Pink to red

535

524

脂质过氧化绿色探针

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

• [1]. Kazuo Tomita, et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci. 2021 Aug 2;22(15):8300.  [Content Brief]

  [2]. Elizabeth M Kenny, et al. Ferroptosis Contributes to Neuronal Death and Functional Outcome After Traumatic Brain Injury. Crit Care Med. 2019 Mar;47(3):410-418.  [Content Brief]

  [3]. Kazunori Yamanaka, et al. A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Advances, 2012, 2, 7894–7900.