MMRi6 

MMRi6 is a Mdm2-MdmX RING domain inhibitor that can disrupt Mdm2-MdmX RING-RING interaction in vitro. MMRi6 inhibits MdmX-stimulated Mdm2 autoubiquitination and Mdm2-MdmX-mediated p53 polyubiquitination in vitro without affecting NEDD4-1 autoubiquitination. MMRi6 induces p53 stabilization and accumulation and induces PARP cleavage in wt-p53 Emu-myc lymphoma cells. MMRi6 inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC₅₀s of approximately 0.5 μM and 3 μM, respectively. MMRi6 can be used for the study of leukemia/lymphoma^[1].

MMRi6 (10 μM) 在体外抑制 Mdm2 自我泛素化和 Mdm2-MdmX 介导的 p53 泛素化^[1]。
MMRi6 (5 μM，8 小时) 在 HCT-8 细胞和 wt-p53 Emu-myc 淋巴瘤细胞中诱导 p53 蛋白积累^[1]。
MMRi6 (0.5 μM，24 小时) 在 wt-p53 Emu-myc 淋巴瘤细胞中诱导 PARP 裂解，但在 p53-null Emu-myc 淋巴瘤细胞中无此作用^[1]。
MMRi6 (0.5-1 μM，72 小时) 抑制 wt-p53 和 p53-null Emu-myc 淋巴瘤细胞的生长，IC₅₀ 值分别为约 0.5 μM 和 3 μM^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

396.27

C₂₁H₁₅Cl₂N₃O

709009-15-4

固体

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Wu W, et al. Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells. Cell Death Dis. 2015 Dec 31;6(12):e2035.  [Content Brief]