1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-10 Receptor
  5. IL-10R alpha
  6. IL-10R alpha 蛋白, Human (HEK293, His)

IL-10R alpha 蛋白, Human (HEK293, His)

目录号: HY-P70692
COA 产品使用指南

IL-10R alpha 蛋白是 IL10 细胞因子表面受体,参与 IL10 介导的炎症及免疫调控,抑制促炎细胞因子的合成。IL-10R alpha 蛋白, Human (HEK293, His) 是由 HEK 293 细胞表达的,具有跨膜区 (V236-L256) ,C 端带有 6*His 标签。

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10 μg ¥1700 In-stock
50 μg ¥5100 In-stock
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描述

IL-10R alpha protein is an IL10 cytokine surface receptor that is involved in IL10-mediated inflammation and immune regulation and inhibits the synthesis of pro-inflammatory cytokines. IL-10R alpha Protein, Human (HEK293, His) is expressed by HEK 293 cells and has a transmembrane region (V236-L256) with a 6*His tag at the C-terminus[1].

研究背景

IL-10R alpha is a ligand-binding subunit of the type II cytokine receptor consisting of 2 alpha and 2 beta subunits and is expressed primarily in hematopoietic cells such as B cells, T cells, NK cells, monocytes and macrophages, but not in non-hematopoietic cells such as fibroblasts or endothelial cells[1].
IL-10R alpha binds to the ligand and leads to a conformational change in the beta subunit, which results in the beta subunit also binding IL-10, forming a heterotetramer that leads to activation of the signalling complex of JAK1 and TYK2 kinases. In this case, JAK1 binds to the alpha subunit and TYK2 binds to the beta subunit, phosphorylating specific tyrosine residues in the intracellular structural domain of IL10R alpha. This further leads to phosphorylation and activation of the transcription factor STAT3, which dimerises STAT3 monomers into the nucleus and induces transcriptional expression of the target gene[1].
IL-10R alpha is involved in suppressing inflammatory responses and Th 1 cell-mediated immune responses, and also regulates neutrophil functional responses. In addition, IL10R alpha-mediated activation of STAT3 also inhibits starvation-induced autophagy[2].

体外研究
(In Vitro)

IL-10R alpha is poorly expressed in human neutrophils while IL-10R alpha expression is significantly up-regulated by incubation in LPS-containing medium for 4 h which allows human neutrophils to respond fully to IL-10[3].
IL-10R alpha is overexpressed in most acute myeloid leukaemia (AML) cells from AML patients and plays an important role in promoting stem cells in leukaemia cells and could be used as a biomarker and potential target for therapeutic intervention in AML[4].
IL-10R alpha can be selectively induced by IFN-γ(10 ng/mL) on human intestinal epithelial cells (T84), so that IL-10R signaling activates protective mechanisms and plays an important role in maintaining and restoring the IEC barrier in vitro[5].

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q13651 (H22-N235)

基因 ID

3587  [NCBI]

中文名
重组人IL-10R alpha蛋白(His)
同用名
Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD210; IL10RA
氨基酸序列

HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTN

分子量

38-59 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献

IL-10R alpha Protein, Human (HEK293, His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-10R alpha Protein, Human (HEK293, His)
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HY-P70692
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