IL-10R alpha 蛋白, Human (HEK293, His)

IL-10R alpha protein is an IL10 cytokine surface receptor that is involved in IL10-mediated inflammation and immune regulation and inhibits the synthesis of pro-inflammatory cytokines. IL-10R alpha Protein, Human (HEK293, His) is expressed by HEK 293 cells and has a transmembrane region (V236-L256) with a 6*His tag at the C-terminus^[1].

IL-10Rα是II型细胞因子受体的配体结合亚基，由2个α和2个β亚基组成，主要在造血细胞中表达，例如B细胞、T细胞、NK细胞、单核细胞和巨噬细胞，但不在非造血细胞中表达。-造血细胞，如成纤维细胞或内皮细胞^[1]。
IL-10R α 与配体结合并导致 β 亚基发生构象变化，从而导致 β 亚基也发生构象变化结合 IL-10，形成异四聚体，从而激活 JAK1 和 TYK2 激酶的信号复合物。在这种情况下，JAK1 与 α 亚基结合，TYK2 与 β 亚基结合，磷酸化 IL10R α 细胞内结构域中的特定酪氨酸残基。这进一步导致转录因子 STAT3 的磷酸化和激活，从而使 STAT3 单体二聚化进入细胞核并诱导靶基因的转录表达^[1]。
IL-10R α 参与抑制炎症反应和 Th 1 细胞介导的免疫反应，还调节中性粒细胞功能反应。此外，IL10R α 介导的 STAT3 激活也会抑制饥饿诱导的自噬^[2]。

IL-10R alpha is poorly expressed in human neutrophils while IL-10R alpha expression is significantly up-regulated by incubation in LPS-containing medium for 4 h which allows human neutrophils to respond fully to IL-10^[3].
IL-10R alpha is overexpressed in most acute myeloid leukaemia (AML) cells from AML patients and plays an important role in promoting stem cells in leukaemia cells and could be used as a biomarker and potential target for therapeutic intervention in AML^[4].
IL-10R alpha can be selectively induced by IFN-γ(10 ng/mL) on human intestinal epithelial cells (T84), so that IL-10R signaling activates protective mechanisms and plays an important role in maintaining and restoring the IEC barrier in vitro^[5].

Human

HEK293

C-6*His

Q13651 (H22-N235)

3587  [NCBI]

HGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTN

38-59 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. Dror S Shouval, et al. Interleukin 10 receptor signaling: master regulator of intestinal mucosal homeostasis in mice and humans. Adv Immunol. 2014;122:177-210.  [Content Brief]

  [2]. J Shi, et al. IL10 inhibits starvation-induced autophagy in hypertrophic scar fibroblasts via cross talk between the IL10-IL10R-STAT3 and IL10-AKT-mTOR pathways. Cell Death Dis. 2016 Mar 10;7(3):e2133.  [Content Brief]

  [3]. L Crepaldi, et al. Up-regulation of IL-10R1 expression is required to render human neutrophils fully responsive to IL-10. J Immunol. 2001 Aug 15;167(4):2312-22.  [Content Brief]

  [4]. Nianci Chen, et al. Targeting of IL-10R on acute myeloid leukemia blasts with chimeric antigen receptor-expressing T cells. Blood Cancer J. 2021 Aug 14;11(8):144.  [Content Brief]

  [5]. Douglas J Kominsky, et al. IFN-γ-mediated induction of an apical IL-10 receptor on polarized intestinal epithelia. J Immunol. 2014 Feb 1;192(3):1267-76.  [Content Brief]