1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors NK Cell CD Proteins Endothelial cell CD Proteins
  4. IL-18 Receptor CD218b/IL-18RAP
  5. IL-18RAP
  6. IL-18R beta /IL-1R7 蛋白, Human (357a.a, HEK293, His)

IL-18R beta /IL-1R7 蛋白, Human (357a.a, HEK293, His)

目录号: HY-P76426
产品使用指南

IL-18 是一种多效性促炎细胞因子,参与诱导炎症介质、调节 NK 细胞和 T 细胞的细胞毒活性以及 Th1 和 Th2 细胞的分化和活化。IL-18 通过其特定的细胞表面受体 IL-18R 结合发出信号,IL-18R 包含两个亚基:IL-18Rα 和 IL-18Rβ。IL-18与质膜上的IL-18受体α(Rα) 和β(Rβ) 链形成信号复合物,诱导多种炎性细胞因子。IL-18R 复合物募集 IL-1R 激酶和 TNF 相关因子 6,诱导核因子 kβ 激酶磷酸化,随后激活核因子 kβ。IL-18R beta 敲低抑制 IL-18 驱动的 C57/BL6 小鼠肿瘤细胞转移。IL-18R beta /IL-1R7 蛋白, Human (357a.a, HEK293, His) 是一种重组蛋白,C 端带有 His 标签,由 358 个氨基酸 (M1-G357) 组成,在 HEK293 细胞中表达。

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描述

IL-18 is a pleiotropic proinflammatory cytokine that is involved in induction of inflammatory mediators, regulation of the cytotoxic activity of NK cells and T cells, and differentiation and activation of both Th1 and Th2 cells. IL-18 signals through its specific cell surface receptor IL-18R, which comprises two subunits: IL-18Rα and IL-18R beta. IL-18 forms a signalling complex with the IL-18 receptor α (Rα) and β (Rβ) chains at the plasma membrane, which induces multiple inflammatory cytokines[2]. IL-18R complex recruits the IL-1R- activating kinase and TNF- associated factor 6, which phosphorylates nuclear factor kβ- inducing kinase, with subsequent activation of NF- kβ[3].IL-18R betaknockdown inhibits IL-18 driven tumor cell metastasis in C57/BL6 mice[4]. IL-18R beta /IL-1R7 Protein, Human (357a.a, HEK293, His) is a recombinant protein with a His label that consists of 358 amino acids (M1-G357) and is produced by HEK293 cells.

研究背景

IL-18R beta (interleukin-18 receptor beta, also known as IL18RAP) is the signal transducing chain of the IL-18R complex and is also a member of the IL-1R family. IL-18R beta is expressed on T-cells, NK cells and dendritic cells, but not commonly expressed in mesenchymal cells[1].
The amino acid sequence of human IL-18R beta protein has low homology for mouse IL-18R beta protein.
IL-18R beta is the receptor of IL-18. IL-18 forms a signaling complex by binding to the IL-18 alpha chain (IL-18Rα). The co-receptor, termed IL-18 receptor beta chain (IL-18Rβ), is recruited to form a high affinity complex[1]. IL-18/IL-18Rα/IL-18Rβ ternary complex formation juxtaposes the intracellular Toll-Interleukin-1 receptor domains of IL-18Rα and IL-18Rβ. Then, the adaptor molecule myeloid differentiation factor 88 (MyD88) is recruited presumably with the aid of TRAM. MyD88 further interacts with IL-1 receptor associating kinase (IRAK) 4 and IRAK1/2 to form the large molecular assembly referred to as Myddosome, which subsequently activates IKK via TRAF6. Finally, the signal activates the NF-κB and mitogen-activated protein kinase pathways7, which upregulate the expression of various inflammatory cytokines[2].
IL-18R beta binds to IL-18 and IL-18 receptor alpha forms a signalling complex induces the expression of various inflammatory cytokines[2]. IL-18R beta knockdown inhibits tumor cell metastasis[4].

生物活性

Measured by its ability to bind biotinylated Cynomolgus IL18 in a functional ELISA.

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

O95256 (M1-G357)

基因 ID

8807  [NCBI]

中文名
重组人白介18 受体 β蛋白
同用名
Interleukin-18 receptor accessory protein; CDw218b; IL-1R7; IL-18R-beta; CD218b
分子量

50-55 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-18R beta /IL-1R7 Protein, Human (357a.a, HEK293, His)
目录号:
HY-P76426
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