2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. TRP Channel
  4. Icilin

Icilin  (Synonyms: AG-3-5)

目录号: HY-11062 纯度: 99.26%
COA 产品使用指南 技术支持

Icilin (AG-3-5) 是瞬时受体电位 M8 (TRPM8) 离子通道的超级激动剂。Icilin 以剂量依赖性方式激活 EGTA 中的 TRPM8（EC50=1.4 μM）。Icilin 是一种“超级冷却剂”。Icilin 通过调节 T 细胞反应来减轻自身免疫性神经炎症。

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Icilin

Icilin Chemical Structure

CAS No. : 36945-98-9

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥363
In-stock
5 mg ￥330
In-stock
10 mg ￥529
In-stock
25 mg ￥1160
In-stock
50 mg ￥2063
In-stock
100 mg ￥2888
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Icilin 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 TRP Channel 亚型特异性产品:

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TRPC TRPM TRPV TRPA TRPML

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Icilin (AG-3-5) is a super-agonist of the transient receptor potential M8 (TRPM8) ion channel. Icilin activates TRPM8 in EGTA in a dose-dependent manner (EC50=1.4 μM). Icilin is a "super-cooling agent" [1][2]. Icilin attenuates autoimmune neuroinflammation through modulation of the T-cell response[3].

IC50 & Target[1]

TRPM8

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
HEK293 IC50
0.058 μM
Compound: 3
Antagonist activity against rat TRPM8 expressed in HEK293 cells assessed as inhibition of icilin-induced intracellular Ca2+ level pre-treated 5 mins before icilin stimulation by Fluo-4-AM dye based spectrofluorimetry
Antagonist activity against rat TRPM8 expressed in HEK293 cells assessed as inhibition of icilin-induced intracellular Ca2+ level pre-treated 5 mins before icilin stimulation by Fluo-4-AM dye based spectrofluorimetry
[PMID: 25935641]
HEK293 EC50
0.11 μM
Compound: 3
Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as induction of intracellular Ca2+ level by Fluo-4-AM dye based spectrofluorimetry
Agonist activity at rat TRPM8 expressed in HEK293 cells assessed as induction of intracellular Ca2+ level by Fluo-4-AM dye based spectrofluorimetry
[PMID: 25935641]
HEK293 EC50
0.19 μM
Compound: icilin
Agonist activity at rat recombinant TRPM8 receptor expressed in HEK293 cells assessed as increase in intracellular calcium concentration by fluorimetric test
Agonist activity at rat recombinant TRPM8 receptor expressed in HEK293 cells assessed as increase in intracellular calcium concentration by fluorimetric test
[PMID: 19162486]
HEK293 EC50
300 nM
Compound: Icilin
Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay
Inhibition of human TRPM8 (unknown origin) expressed in HEK293 cells assessed as inhibition of WS12-induced Ca2+ elevation by FLIPR assay
[PMID: 24080460]
体外研究
(In Vitro)

Icilin 诱发的 TRPM8 电流显示可变的激活动力学和 Ca2+ 依赖性。Icilin 诱发的电流表现出高度可变的潜伏期和明显的脱敏[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Icilin 相关抗体:

Cell Viability Assay[1]

Cell Line: TRPM8-expressing oocytes or HEK293 cells
Concentration: 10 μM
Incubation Time: 3 minutes for TRPM8-expressing oocytes; 1 minute for HEK293 cells
Result: Activated membrane currents with variable delay of onset in voltage-clamped TRPM8-expressing oocytes or HEK293 cells.
体内研究
(In Vivo)

Icilin 是一种瞬时受体电位阳离子通道亚家族 M (TRPM8) 激动剂，可在大鼠和小鼠中产生行为激活。Icilin (3 mg/kg；sc) 诱导小鼠“Wet dog shake”[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: The C57BL/6 mice 9–10 weeks (adult, 26-30 g) or 24 months (aged, 35-42g)[2].
Dosage: 3 mg/kg
Administration: Injected s.c.
Result: Produced vivid and quantifiable shaking behaviors (“wet-dog shakes”), which were TRPM8-dependent. 
分子量

311.29

Formula

C16H13N3O4
CAS 号
性状

固体

颜色

Light yellow to yellow

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 100 mg/mL (321.24 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2124 mL 16.0622 mL 32.1244 mL
5 mM 0.6425 mL 3.2124 mL 6.4249 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.26%

COA (295 KB) HNMR (179 KB) RP-HPLC (169 KB) LCMS (228 KB)

产品使用指南 (1538 KB)
参考文献

Icilin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2124 mL 16.0622 mL 32.1244 mL 80.3110 mL
5 mM 0.6425 mL 3.2124 mL 6.4249 mL 16.0622 mL
10 mM 0.3212 mL 1.6062 mL 3.2124 mL 8.0311 mL
15 mM 0.2142 mL 1.0708 mL 2.1416 mL 5.3541 mL
20 mM 0.1606 mL 0.8031 mL 1.6062 mL 4.0155 mL
25 mM 0.1285 mL 0.6425 mL 1.2850 mL 3.2124 mL
30 mM 0.1071 mL 0.5354 mL 1.0708 mL 2.6770 mL
40 mM 0.0803 mL 0.4016 mL 0.8031 mL 2.0078 mL
50 mM 0.0642 mL 0.3212 mL 0.6425 mL 1.6062 mL
60 mM 0.0535 mL 0.2677 mL 0.5354 mL 1.3385 mL
80 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0039 mL
100 mM 0.0321 mL 0.1606 mL 0.3212 mL 0.8031 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Icilin36945-98-9AG-3-5TRP ChannelTransient receptor potential channelssupertransientreceptorpotentialTRPM8ionchannel ,coolingautoimmuneneuroinflammationT-cellresponseInhibitorinhibitorinhibit

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