2. Cell Cycle/DNA Damage Cytoskeleton Protein Tyrosine Kinase/RTK Autophagy Apoptosis
  3. Microtubule/Tubulin Bcr-Abl CRISPR/Cas9 Autophagy Apoptosis
  4. Nocodazole

Nocodazole  (Synonyms: 诺考达唑; Oncodazole; R17934)

目录号: HY-13520 纯度: 99.66%
COA 产品使用指南

摩尔计算器

Nocodazole (Oncodazole) 是快速可逆的 microtubule 抑制剂。 Nocodazole与β-微管蛋白结合并破坏微管组装/拆卸动力学，从而防止有丝分裂并诱导肿瘤细胞凋亡。Nocodazole 抑制 Bcr-Abl，增强 CRISPR/Cas9 的活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Nocodazole Chemical Structure

Nocodazole Chemical Structure

CAS No. : 31430-18-9

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥363
In-stock
5 mg ￥330
In-stock
10 mg ￥515
In-stock
50 mg ￥1989
In-stock
100 mg ￥3320
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Nocodazole 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 53 篇科研文献

IF
WB

    Nocodazole purchased from MCE. Usage Cited in: Science. 2022 Nov 18;378(6621):eabq7361.  [Abstract]

    Representative time-lapse images (z-stack) showing microtubule nucleation after nocodazole washout in live human oocytes. Gray, microtubules (GFP-MAP4); magenta, kinetochores (mScarlet-CENPB).
    Flow diagram shows the sequence of experiments. Arrow indicates timelapse imaging initiation. Time is given as hours: minutes after nocodazole washout.

    Nocodazole purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).

    Nocodazole purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ( (-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Nocodazole (Oncodazole) is a rapidly-reversible inhibitor of microtubule. Nocodazole binds to β-tubulin and disrupts microtubule assembly/disassembly dynamics, which prevents mitosis and induces apoptosis in tumor cells. Nocodazole inhibits Bcr-Abl, and activates CRISPR/Cas9.

    IC50 & Target[1]

    Abl

    91 nM (Kd)

    ABL(E255K)

    120 nM (Kd)

    ABL(T315I)

    170 nM (Kd)

    BRAF

    1.8 μM (Kd)

    BRAF(V600E)

    1.1 μM (Kd)

    c-KIT

    1.6 μM (Kd)

    MEK1

    1.7 μM (Kd)

    MEK2

    1.6 μM (Kd)

    MET

    1.7 μM (Kd)

    PI3Kγ

    1.5 μM (Kd)

    Microtubule/Tubulin

     

    CRISPR/Cas9

     

    体外研究
    (In Vitro)

    Nocodazole exhibits good affinity toward c-KIT, with a Kd value of 1.6 μM in highly malignant human cancer cells. Nocodazole displays good binding affinity toward the components of the mitogen-activated protein kinase (MAPK) pathway, such as BRAF (Kd=1.8 μM), BRAF(V600E) (Kd=1.1 μM), MEK1 (Kd=1.7 μM), and MEK2 (Kd=1.6 μM)[1]. Nocodazole has the highest affinity for αβIV and the lowest affinity for αβIII[2].
    Nocodazole (1 nM) induces apoptosis of COLO 205 cancer cells[3].
    Nocodazole (≥ 30 µg/mL) significantly increases the percentage of annexin-V-binding cells without significantly modifying average forward scatter of human erythrocytes[4].
    In CHO cells, the addition of 1 nM Nocodazole, a concentration that suppresses microtubule dynamics, slows migration and increases the frequency and duration of resting states, but the directionality of the cells is maintained. In contrast to the effects of the low drug concentration, the addition of 70 nM Nocodazole, a concentration that eliminates the microtubule network, causes cells to move much more randomly, i.e., the directionality of the cells toward the wound is lost[6].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Nocodazole 相关抗体:
    体内研究
    (In Vivo)

    Nocodazole (5 mg/kg/three times per week, i.p.) has antitumor effects in athymic mice bearing COLO 205 tumor xenografts. Nocodazole (1 nM) + R-41400 dramatically increase the levels of p21/CIP1 and p27/KIP1 protein in the tumor tissues[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    301.32

    Formula

    C14H11N3O3S
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    中文名称

    诺考达唑
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 20 mg/mL (66.37 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.3187 mL 16.5937 mL 33.1873 mL
    5 mM 0.6637 mL 3.3187 mL 6.6375 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.90 mM); 澄清溶液

      此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2 mg/mL (6.64 mM); 澄清溶液

      此方案可获得 ≥ 2 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： 50% PEG300    50% Saline

      Solubility: 5 mg/mL (16.59 mM); 悬浊液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.66%

    COA (193 KB) HNMR (254 KB) LCMS (268 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [3]

    Proteins are loaded at 50 μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    COLO 205 cells are grown in RPMI 1640 supplemented with 10% FCS. Cells are harvested through two consecutive trypsinizations, centrifuged at 300×g; for 5 min, washed twice, and resuspended in sterile phosphate-buffered saline (PBS). Cells (5×105) in 0.1 mL are injected subcutaneously between the scapulae of each nude mouse. After transplantation, tumor size is measured with calipers, and the tumor volume is estimated. Once tumors reach a mean size of 200 mm3, animals receive intraperitoneal injections of DMSO (25 μL), R-41400 (50 mg/kg), nocodazole (5 mg/kg), or R-41400 + nocodazole three times per week for 6 wk.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Nocodazole 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3187 mL 16.5937 mL 33.1873 mL 82.9683 mL
    5 mM 0.6637 mL 3.3187 mL 6.6375 mL 16.5937 mL
    10 mM 0.3319 mL 1.6594 mL 3.3187 mL 8.2968 mL
    15 mM 0.2212 mL 1.1062 mL 2.2125 mL 5.5312 mL
    20 mM 0.1659 mL 0.8297 mL 1.6594 mL 4.1484 mL
    25 mM 0.1327 mL 0.6637 mL 1.3275 mL 3.3187 mL
    30 mM 0.1106 mL 0.5531 mL 1.1062 mL 2.7656 mL
    40 mM 0.0830 mL 0.4148 mL 0.8297 mL 2.0742 mL
    50 mM 0.0664 mL 0.3319 mL 0.6637 mL 1.6594 mL
    60 mM 0.0553 mL 0.2766 mL 0.5531 mL 1.3828 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nocodazole31430-18-9Oncodazole R17934诺考达唑R17934R 17934R-17934Microtubule/TubulinBcr-AblCRISPR/Cas9AutophagyApoptosisInhibitorinhibitorinhibit

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