2. MAPK/ERK Pathway Apoptosis Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Cell Cycle/DNA Damage Epigenetics
  3. p38 MAPK Bcl-2 Family Caspase Reactive Oxygen Species (ROS) PARP Apoptosis
  4. MAPK-IN-5

MAPK-IN-5 是一种强效的 MAPK 抑制剂。MAPK-IN-5 对 Hela 细胞的 IC50 值为 1.35 μM。MAPK-IN-5 可通过 MAPK 途径诱导 ROS 介导的 DNA 损伤和线粒体细胞凋亡 (apoptosis)。MAPK-IN-5显著抑制 HeLa 细胞集落形成，减少活细胞数量，抑制细胞迁移，阻滞周期于 G2/M 期。MAPK-IN-5 可用于宫颈癌的研究。

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MAPK-IN-5

MAPK-IN-5 Chemical Structure

CAS No. : 2579683-39-7

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MAPK-IN-5 相关抗体

Top Publications Citing Use of Products

查看 p38 MAPK 亚型特异性产品:

查看所有亚型
p38 MAPK p38α p38β MAPK13 p38δ p38γ

查看 Bcl-2 Family 亚型特异性产品:

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3

查看 Caspase 亚型特异性产品:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

查看 PARP 亚型特异性产品:

查看所有亚型
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

MAPK-IN-5 is a potent MAPK inhibitor with an IC50 of 1.35 μM against HeLa cells. MAPK-IN-5 inhibits HeLa cell proliferation by inducing ROS-mediated DNA damage and mitochondrial apoptosis via the MAPK pathway. MAPK-IN-5 significantly inhibits colony formation, reduces the number of live cells, suppresses cell migration, and causes cell cycle arrest in the G2/M phase in HeLa cells. MAPK-IN-5 can be used for the study of cervical cancer[1].

IC50 & Target[1]

p38

 

Caspase-3

 

Caspase-9

 

Bcl-2

 

PARP-1

 

体外研究
(In Vitro)

MAPK-IN-5 (Compound 3h) (5-10 μM, 48 h) 可显著抑制HeLa细胞的集落形成并增加死亡细胞数量[1]。
MAPK-IN-5 (5-10 μM, 0-48 h) 对 HeLa 细胞的迁移具有显著抑制作用[1]。
MAPK-IN-5 (5-10 μM, 24 h) 可诱导 HeLa 细胞发生 G2/M 期阻滞[1]。
MAPK-IN-5 (5-10 μM, 48 h) 可通过激活线粒体凋亡通路诱导 HeLa 细胞凋亡[1]。
MAPK-IN-5 (5-10 μM, 48 h) 可促进 HeLa 细胞中 ROS 的生成，引发 DNA 损伤并调节 MAPK 信号通路[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

MAPK-IN-5 相关抗体:

Immunofluorescence[1]

Cell Line: HeLa cells
Concentration: 5 and 10 μM
Incubation Time: 48 h
Result: The number of live cells gradually decreased, while the number of dead cells increased.
Dose-dependently increased the intracellular ROS level.
Increased the fluorescence intensity of γ-H2AX in the nuclei of HeLa cells.

Cell Migration Assay [1]

Cell Line: HeLa cells
Concentration: 5 and 10 μM
Incubation Time: 0, 24, 48 h
Result: The migration rate of HeLa cells was reduced to 20.75% at 10 μM.

Cell Cycle Analysis[1]

Cell Line: HeLa cells
Concentration: 5 and 10 μM
Incubation Time: 24 h
Result: The proportion of cells in the G1 phase gradually decreased, while the proportion of cells in the G2/M phase significantly accumulated.

Apoptosis Analysis[1]

Cell Line: HeLa cells
Concentration: 5 and 10 μM
Incubation Time: 48 h
Result: The total apoptosis rate of HeLa cells was 37.63% at 10 μM.
Cell shrinkage, intensified nuclear staining, and even apoptotic features such as nuclear condensation and fragmentation were observed.

Western Blot Analysis[1]

Cell Line: HeLa cells
Concentration: 5 and 10 μM
Incubation Time: 48 h
Result: Decreased the levels of Bcl-2, Caspase-3, Caspase-9, and PARP-1.
Increased the level of Bax and Cleaved PARP-1.
Dose-dependently inhibited the levels of phosphorylated ERK1/2 (p-ERK1/2) and phosphorylated JNK (p-JNK), while upregulating the phosphorylation of p38.
Had no effect on the total protein levels of ERK1/2, JNK, and p38.
分子量

594.58

Formula

C30H29F3N6O4
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

MAPK-IN-5 相关分类

  • 摩尔计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MAPK-IN-52579683-39-7p38 MAPKBcl-2 FamilyCaspaseReactive Oxygen Species (ROS)PARPApoptosispoly ADP ribose polymerase Cervical cancerErlotinibMAPK signaling pathwayTriazoleHela cellsInhibitorinhibitorinhibit

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