2. Academic Validation
  3. Identification and evaluation of Nordihydroguaiaretic acid (NDGA) as an active traditional Chinese medicine compound inhibiting the 3C-like protease of feline infectious peritonitis virus

Identification and evaluation of Nordihydroguaiaretic acid (NDGA) as an active traditional Chinese medicine compound inhibiting the 3C-like protease of feline infectious peritonitis virus

  • Vet Microbiol. 2025 Sep 15:310:110730. doi: 10.1016/j.vetmic.2025.110730.
Wei Yuan 1,  Xiaoen Pang 1,  Jingru Zhu 1,  Zan Chen 1,  Shuna Li 1,  Zhibo Yang 1,  Xiaohu Zhang 1,  Changbin Jin 1,  Xintong Huang 1,  Yu Shen 1,  Huarong Fang 1,  Jiahao Lin 2,  Yueping Zhang 3
Affiliations

Affiliations

  • 1 National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • 2 National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China; Beijing Zhongnongda Veterinary Hospital Co., Ltd., Beijing 100193, China; Teaching Animal Hospital, China Agricultural University, Beijing 100193, China. Electronic address: jiahao_lin@cau.edu.cn.
  • 3 National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: zhangyueping@cau.edu.cn.
PMID: 40976146 DOI: 10.1016/j.vetmic.2025.110730
Abstract

Feline infectious peritonitis (FIP) is a systemic and fatal disease that poses a serious threat to feline population, underscoring the urgent need for development of novel Antiviral drugs. The FIP is caused by the feline infectious peritonitis virus (FIPV), a highly pathogenic feline coronavirus (FCoV), and 3C-like protease (3CLpro) is a critical drug target in coronaviruses. Here, we employed structure-based in silico screening and identified Nordihydroguaiaretic acid (NDGA), a bioactive compound used in traditional Chinese medicine, as a potential FIPV 3CLpro inhibitor. Enzymatic assays confirmed that NDGA effectively inhibits FIPV 3CLpro activity. Furthermore, cellular assays demonstrated that NDGA suppresses FIPV replication. Additionally, in a feline challenge model with an FCoV-II strain, NDGA treatment prolonged survival and improved clinical outcomes. These results support NDGA as a potential lead compound for FIP therapy.

Keywords

3C-like protease; Antiviral; Feline infectious peritonitis; Nordihydroguaiaretic acid; Traditional Chinese medicine.

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