Venetoclax (Synonyms: 维奈妥拉; ABT-199; GDC-0199; RG7601)

目录号: HY-15531 纯度: 99.95%: Data Sheet SDS COA 产品使用指南
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Venetoclax (ABT-199; GDC-0199) 是一种高效，有选择性和口服有效的 Bcl-2 抑制剂，K_i 小于0.01 nM。Venetoclax 可以诱导自噬 (autophagy) 作用。

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥802	In-stock
5 mg	￥600	In-stock
10 mg	￥840	In-stock
50 mg	￥1080	In-stock
100 mg	￥1400	In-stock
500 mg	￥3680	In-stock
1 g	￥5840	In-stock
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Customer Review

Other Forms of Venetoclax:

MCE 顾客使用本产品发表的 208 篇科研文献

Cell Viability Assay

: Venetoclax purchased from MCE. Usage Cited in: Cancer Cell. 2024 Apr 8;42(4):552-567.e6. [Abstract]; Venetoclax (100 nM; 12-24 h). Western blot of the phosphorylation level of S6K1 upon the treatment of pegaspargase, venetoclax, or combination in both Nalm6 and 697 cells.

: Venetoclax purchased from MCE. Usage Cited in: Mol Cancer. 2024 Oct 31;23(1):243. [Abstract]; Venetoclax (10-160 μM; 12-72 h) combined with Cisplatin (2 μg/mL; 24-72 h) and Hydroxychloroquine (12.5-50 μM; 12-72 h) significantly inhibits the growth of H446/cDDP cells.

: Venetoclax purchased from MCE. Usage Cited in: Mol Cancer. 2024 Sep 20;23(1):204. [Abstract]; Venetoclax (0.1 µM; 24-96 h) significantly inhibits the proliferation of EG cells derived from FL (fetal liver) and CB (cord blood), and demonstrated enhanced inhibitory effects when combined with Trametinib (0.1 µM; 24-96 h).

: Venetoclax purchased from MCE. Usage Cited in: Cancer Cell. 2020 Dec 14;38(6):872-890.e6. [Abstract]; Cell viability of parental and venetoclax-resistant Molm-13 cells treated with MIK665 and +/− 1.3 μM venetoclax at 72 h.

: Venetoclax purchased from MCE. Usage Cited in: Nucleic Acids Res. 2020 Dec 16;48(22):12727-12745. [Abstract]; Co-immunoprecipitation assay and western blot showing immunoprecipitated Bcl-2, immunoblotted COX5A and IgG control lane. Input showing S70pBcl2, Bcl-2, COX5A, β-actin of Jurkat cells following treatment with venetoclax (2µM) for 24 hours.

: Venetoclax purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 10;503(3):1214-1220. [Abstract]; Bcl-2 family protein expression is assessed by western blotting in CNE-2 and 5-8F cells after treatment with ABT-199 for 48 h.

: Venetoclax purchased from MCE. Usage Cited in: Cancer Cell. 2017 Oct 9;32(4):490-505.e10. [Abstract]; Immunoblot for BAX, BCL-2, BCL-XL, and MCL-1 from immunoprecipitates of BAX from OCI-AML3 cells treated with Venetoclax (1.25 μM), BTSA1 (1.25 μM), or in combination after 2.5 hr, and western blot detection for BAX, BCL-2, BCL-XL, and MCL-1.

: Venetoclax purchased from MCE. Usage Cited in: Nat Cell Biol. 2017 Oct;19(10):1226-1236. [Abstract]; ABT-199 inhibits various forms of oncogenic GLI activation.

: Venetoclax purchased from MCE. Usage Cited in: Translational Cancer Research. Vol 6, No 4. 2017.; ABT-199 regulates p53/p21 signaling to induce G2/M phase arrest in DOHH2 cells. Representative blots of CDK1/cdc2, cyclin B1, p21 and p53 in DOHH2 cells treated with ABT-199 at 0.1 and 1 μM for 24 h. β-actin is used as the internal control.

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Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Venetoclax (ABT-199; GDC-0199) is a highly potent, selective and orally bioavailable Bcl-2 inhibitor with a K_i of less than 0.01 nM. Venetoclax induces autophagy^[1]^[2]^[3].

IC₅₀ & Target^[1]

Bcl-2

0.01 nM (Ki)

Bcl-xL

48 nM (Ki)

Bcl-W

245 nM (Ki)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.5 μM Compound: ABT-199	Cytotoxicity against human A549 cells assessed as inhibition of cell growth Cytotoxicity against human A549 cells assessed as inhibition of cell growth	[PMID: 38909705]
Cancer cell lines	GI₅₀	2.6 μM Compound: ABT-199	Cytotoxicity against human renal cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human renal cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	2.7 μM Compound: ABT-199	Cytotoxicity against human non-small cell lung cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human non-small cell lung cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	2.77 μM Compound: ABT-199	Cytotoxicity against human breast cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human breast cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	2.85 μM Compound: ABT-199	Cytotoxicity against human colon cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human colon cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	3.33 μM Compound: ABT-199	Cytotoxicity against human ovarian cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human ovarian cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	3.35 μM Compound: ABT-199	Cytotoxicity against human CNS cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human CNS cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
Cancer cell lines	GI₅₀	3.51 μM Compound: ABT-199	Cytotoxicity against human prostate cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human prostate cancer cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
DMS-79	EC₅₀	4.62 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human DMS 79 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human DMS 79 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
EOL1	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human EOL1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human EOL1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
GDM-1	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human GDM-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human GDM-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
HEK-293T	IC₅₀	> 20 μM Compound: ABT-199	Cytotoxicity against HEK293T cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay	[PMID: 34228434]
HEL	EC₅₀	3.37 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human HEL harboring JAK2 V617F mutant assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human HEL harboring JAK2 V617F mutant assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
HL-60	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human HL-60 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human HL-60 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
HL-60	EC₅₀	8.1 μM Compound: ABT-199	Induction of apoptosis in human HL-60 cells measured after 48 hrs by FITC-Annexin V/PS staining based Induction of apoptosis in human HL-60 cells measured after 48 hrs by FITC-Annexin V/PS staining based	[PMID: 33197310]
HL-60	IC₅₀	0.077 μM Compound: ABT-199	Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay	[PMID: 34228434]
HeLa	IC₅₀	> 20 μM Compound: ABT-199	Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay	[PMID: 34228434]
K562	EC₅₀	2.1 μM Compound: ABT199	Cytotoxicity against human K562 cells assessed as cell survival measured after 72 hrs by MTT assay Cytotoxicity against human K562 cells assessed as cell survival measured after 72 hrs by MTT assay	[PMID: 34216984]
KG-1	EC₅₀	0.85 μM Compound: ABT-199	Induction of apoptosis in human KG-1 cells measured after 48 hrs by FITC-Annexin V/PS staining based Induction of apoptosis in human KG-1 cells measured after 48 hrs by FITC-Annexin V/PS staining based	[PMID: 33197310]
KG-1	EC₅₀	1.2 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human KG-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human KG-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
Kasumi 1	EC₅₀	1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human Kasumi 1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human Kasumi 1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
Leukemia cell	GI₅₀	2.11 μM Compound: ABT-199	Cytotoxicity against human leukemia cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human leukemia cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
MCF7	EC₅₀	1.35 μM Compound: ABT-199	Induction of apoptosis in human MCF7 cells measured after 48 hrs by FITC-Annexin V/PS staining based Induction of apoptosis in human MCF7 cells measured after 48 hrs by FITC-Annexin V/PS staining based	[PMID: 33197310]
ML-2	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human ML-2 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human ML-2 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
MOLM-13	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human MOLM-13 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human MOLM-13 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
MOLM-16	EC₅₀	0.04 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human MOLM16 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human MOLM16 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
MOLT-4	EC₅₀	8 nM Compound: ABT-199, GDC-0199	Antiproliferative activity against Bcl-2 dependent human MOLT-4 cells assessed as reduction in cell viability by measuring effect concentration by CellTiter-Glo reagent based assay Antiproliferative activity against Bcl-2 dependent human MOLT-4 cells assessed as reduction in cell viability by measuring effect concentration by CellTiter-Glo reagent based assay	[PMID: 25787766]
MOLT-4	EC₅₀	> 5000 nM Compound: 23	Antiproliferative activity against human MOLT4 cells in presence of 10% human serum Antiproliferative activity against human MOLT4 cells in presence of 10% human serum	[PMID: 28926247]
MOLT-4	EC₅₀	> 5000 nM Compound: ABT-199, GDC-0199	Antiproliferative activity against Bcl-xl dependent human MOLT-4 cells assessed as reduction in cell viability by measuring effect concentration by CellTiter-Glo reagent based assay Antiproliferative activity against Bcl-xl dependent human MOLT-4 cells assessed as reduction in cell viability by measuring effect concentration by CellTiter-Glo reagent based assay	[PMID: 25787766]
MOLT-4	IC₅₀	2823 nM Compound: 1c; ABT-199	Inhibition of cell growth in human MOLT-4 cells incubated for 2 days by CellTiter-Glo luminescent cell viability assay Inhibition of cell growth in human MOLT-4 cells incubated for 2 days by CellTiter-Glo luminescent cell viability assay	[PMID: 38695063]
MV4-11	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human MV4-11 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human MV4-11 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
MV4-11	IC₅₀	0.44 μM Compound: 7; ABT199	Antiproliferative activity against human MV4-11 cells expressing high BCL-2 assessed as cell viability measured after 72 hrs by Cell Counting Kit-8 assay Antiproliferative activity against human MV4-11 cells expressing high BCL-2 assessed as cell viability measured after 72 hrs by Cell Counting Kit-8 assay	[PMID: 38913996]
Melanoma cell	GI₅₀	1.75 μM Compound: ABT-199	Cytotoxicity against human melanoma cells assessed as cell growth inhibition after 48 hrs by SRB assay Cytotoxicity against human melanoma cells assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 27994761]
NCI-H1048	EC₅₀	2.7 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H1048 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H1048 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H1417	EC₅₀	4.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H1417 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H1417 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H1836	EC₅₀	5 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H1836 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H1836 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H187	EC₅₀	0.85 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H187 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H187 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H211	EC₅₀	0.33 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H211 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H211 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H345	EC₅₀	1.2 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H345 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H345 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H446	EC₅₀	2.4 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H446 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H446 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H69	EC₅₀	3.86 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H69 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H69 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NCI-H889	EC₅₀	0.015 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NCI-H889 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NCI-H889 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
NOMO-1	EC₅₀	> 5 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human NOMO-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human NOMO-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
OCI-AML-3	EC₅₀	6.8 μM Compound: ABT-199	Induction of apoptosis in human OCI-AML-3 cells measured after 48 hrs by FITC-Annexin V/PS staining based Induction of apoptosis in human OCI-AML-3 cells measured after 48 hrs by FITC-Annexin V/PS staining based	[PMID: 33197310]
OCI-AML-3	EC₅₀	> 5 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human OCI-AML-3 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human OCI-AML-3 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
OCI-AML-5	EC₅₀	0.11 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human OCI-AML5 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human OCI-AML5 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
OCI-AML2	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human OCI-AML2 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human OCI-AML2 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
Pfeiffer	IC₅₀	6.26 μM Compound: 7; ABT199	Antiproliferative activity against human Pfeiffer cells expressing high BCL-2/BFL-1 assessed as cell viability measured after 72 hrs by Cell Counting Kit-8 assay Antiproliferative activity against human Pfeiffer cells expressing high BCL-2/BFL-1 assessed as cell viability measured after 72 hrs by Cell Counting Kit-8 assay	[PMID: 38913996]
RPMI-8226	IC₅₀	2.7 μM Compound: ABT-199	Growth inhibition of human RPMI8226 cells after 72 hrs by MTT assay Growth inhibition of human RPMI8226 cells after 72 hrs by MTT assay	[PMID: 30594434]
RS4-11	EC₅₀	3 nM Compound: ABT-199	Induction of apoptosis in human RS4-11 cells Induction of apoptosis in human RS4-11 cells	[PMID: 32563811]
RS4-11	EC₅₀	8 nM Compound: 23	Antiproliferative activity against human RS4:11 cells in presence of 10% human serum Antiproliferative activity against human RS4:11 cells in presence of 10% human serum	[PMID: 28926247]
RS4-11	IC₅₀	0.0043 μM Compound: ABT-199	Cytotoxicity against human RS4-11 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay Cytotoxicity against human RS4-11 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay	[PMID: 34228434]
RS4-11	IC₅₀	0.006 μM Compound: ABT-199; 1	Antiproliferative activity against human RS4:11 cells after 72 hrs by MTS assay Antiproliferative activity against human RS4:11 cells after 72 hrs by MTS assay	[PMID: 30278333]
RS4-11	IC₅₀	0.33 μM Compound: ABT-199	Cytotoxicity against human RS4:11 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human RS4:11 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 29453135]
RS4-11	IC₅₀	1.5 nM Compound: ABT-199	Cytotoxicity against human RS4:11 cells assessed as reduction in cell viability Cytotoxicity against human RS4:11 cells assessed as reduction in cell viability	[PMID: 29407973]
RS4-11	IC₅₀	1.8 nM Compound: 6; ABT-199	Antiproliferative activity against human RS4-11 cells assessed as inhibition of cell proliferation measured after 72 hrs by CCK8 assay Antiproliferative activity against human RS4-11 cells assessed as inhibition of cell proliferation measured after 72 hrs by CCK8 assay	[PMID: 34536651]
RS4-11	IC₅₀	110 nM Compound: 1c; ABT-199	Inhibition of cell growth in human RS4-11 cells harboring Bcl-2 G101V mutant incubated for 2 days by CellTiter-Glo luminescent cell viability assay Inhibition of cell growth in human RS4-11 cells harboring Bcl-2 G101V mutant incubated for 2 days by CellTiter-Glo luminescent cell viability assay	[PMID: 38695063]
RS4-11	IC₅₀	4.3 nM Compound: 1c; ABT-199	Inhibition of cell growth in human RS4-11 cells incubated for 2 days by CellTiter-Glo luminescent cell viability assay Inhibition of cell growth in human RS4-11 cells incubated for 2 days by CellTiter-Glo luminescent cell viability assay	[PMID: 38695063]
SET-2	EC₅₀	3.33 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human SET2 harboring JAK2 V617F mutant assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human SET2 harboring JAK2 V617F mutant assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
SIG-M5	EC₅₀	0.1 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human SIG-M5 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human SIG-M5 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
SKM-1	EC₅₀	2.53 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human SKM-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human SKM-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
THP-1	EC₅₀	0.82 μM Compound: ABT-199, GDC-0199	Antiproliferative activity against human THP-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay Antiproliferative activity against human THP-1 assessed as reduction in cell viability incubated for 48 hrs by CellTiter-Glo cell vaibility assay	[PMID: 25787766]
Toledo	IC₅₀	0.011 μM Compound: ABT-199; 1	Antiproliferative activity against human Toledo cells after 72 hrs by MTS assay Antiproliferative activity against human Toledo cells after 72 hrs by MTS assay	[PMID: 30278333]
U-266	IC₅₀	94 μM Compound: ABT-199	Growth inhibition of human U266 cells after 72 hrs by MTT assay Growth inhibition of human U266 cells after 72 hrs by MTT assay	[PMID: 30594434]

体外研究
(In Vitro)

Venetoclax (ABT-199) 有效杀死 FL5.12-BCL-2 细胞 (EC₅₀=4 nM)，Venetoclax (ABT-199) 对 FL5.12-表现出更弱的活性 BCL-XL 细胞 (EC₅₀=261 nM)。ABT-199 在细胞哺乳动物双杂交试验中也表现出选择性，它会破坏 BCL-2-BIM 复合物 (EC₅₀=3 nM)，但对 BCL-XL 的效果要差得多-BCL-XS (EC₅₀=2.2 μM) 或 MCL-1-NOXA 复合物^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

在源自 RS4;11 细胞 (ALL) 的异种移植物模型中，单次口服剂量为 12.5 mg/kg 后，Venetoclax (ABT-199) 导致最大肿瘤生长抑制 (TGI_max=47%) (P<0.001) 和肿瘤生长延迟 (TGD=26%) (P<0.05)^[1]。
用 Venetoclax (ABT-199) 100 mg/kg 处理 4 天已建立的异种移植 (T-ALL 细胞系 LOUCY 的小鼠异种移植模型) 肿瘤可显著降低白血病负担^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

868.44

Formula

C₄₅H₅₀ClN₇O₇S

CAS 号

1257044-40-8

性状

固体

颜色

Light yellow to yellow

中文名称

维奈妥拉

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 77.5 mg/mL (89.24 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

Ethanol 中的溶解度 : < 1 mg/mL (insoluble)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.1515 mL	5.7575 mL	11.5149 mL
5 mM	0.2303 mL	1.1515 mL	2.3030 mL
10 mM	0.1151 mL	0.5757 mL	1.1515 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% Ethanol 60% Phosal 50 PG 30% PEG400
Solubility: 20 mg/mL (23.03 mM); 悬浊液; 超声助溶
方案二

请依序添加每种溶剂： 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 5 mg/mL (5.76 mM); 悬浊液; Need ultrasonic and warming and heat to 49°C
方案三

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (2.88 mM); 悬浊液; 超声助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案四

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.88 mM); 悬浊液; 超声加热助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 45% PEG300 5% Tween-80 50% Saline
Solubility: 10 mg/mL (11.51 mM); 悬浊液; 超声助溶
方案二

请依序添加每种溶剂： 15% Cremophor EL 85% Saline
Solubility: 10 mg/mL (11.51 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.95%

选择批次：

Data Sheet (643 KB) SDS (558 KB)

COA (287 KB) HNMR (220 KB) LCMS (139 KB) 元素分析报告 (210 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Souers AJ, et al. ABT-199, a potent and selective BCL-2 inhibitor, achieves antitumor activity while sparing platelets. Nat Med. 2013 Feb;19(2):202-8. [Content Brief]

[2]. Peirs S, et al. ABT-199 mediated inhibition of BCL-2 as a novel therapeutic strategy in T-cell acute lymphoblastic leukemia. Blood. 2014 Dec 11;124(25):3738-47. [Content Brief]

[3]. Bi C, et al. Inhibition of 4EBP phosphorylation mediates the cytotoxic effect of mechanistic target of rapamycin kinase inhibitors in aggressive B-cell lymphomas. Haematologica. 2017 Apr;102(4):755-764. [Content Brief]

Cell Assay ^[1]	RS4;11 cells are seeded at 50,000 per well in 96-well plates and treated with compounds diluted in half-log steps starting at 1 μM and ending at 0.00005 μM. All other leukemia and lymphoma cell lines are seeded at 15,000-20,000 cells per well in the appropriate medium and incubated with Venetoclax or Navitoclax for 48 h. Effects on proliferation are determined using Cell TiterGlo reagent. EC₅₀ values are determined by nonlinear regression analysis of the concentration-response data. Mouse FL5.12-BCL-2 and FL5.12-BCL-XL cells are propagated and assessed. Bak^-/- Bax^-/- double knockout mouse embryonic fibroblasts are seeded into 96-well microtiter plates at 5,000 cells per well in DMEM supplemented with 10% FBS. Venetoclax (ABT-199) in the same culture medium is added in half-log dilutions starting at 5 μM. The cells are then incubated at 37°C (5% CO₂) for 48 h, and the effects on proliferation are determined using Cell TiterGlo reagent^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice^[2] Nonobese diabetic/severe combined immunodeficient γ (NSG) mice are injected at 6 weeks of age in the tail vein with 150 µL phosphate-buffered saline containing 5×10⁶ luciferase-labeled LOUCY cells. At regular time points, the bioluminescence is measured using the IVIS Lumina II imaging system. At 6 weeks, the cells are engrafted and the mice are randomly divided into 2 groups (with an equal number of males and females in both groups), and the treatment is started on day 0. Mice are treated with Venetoclax (ABT-199) 100 mg/kg body weight or with vehicle via oral gavage for 4 consecutive days. At days 0, 2, and 4 the bioluminescene is measured. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Souers AJ, et al. ABT-199, a potent and selective BCL-2 inhibitor, achieves antitumor activity while sparing platelets. Nat Med. 2013 Feb;19(2):202-8. [Content Brief] [2]. Peirs S, et al. ABT-199 mediated inhibition of BCL-2 as a novel therapeutic strategy in T-cell acute lymphoblastic leukemia. Blood. 2014 Dec 11;124(25):3738-47. [Content Brief] [3]. Bi C, et al. Inhibition of 4EBP phosphorylation mediates the cytotoxic effect of mechanistic target of rapamycin kinase inhibitors in aggressive B-cell lymphomas. Haematologica. 2017 Apr;102(4):755-764. [Content Brief]

Venetoclax 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1515 mL	5.7575 mL	11.5149 mL	28.7873 mL
	5 mM	0.2303 mL	1.1515 mL	2.3030 mL	5.7575 mL
	10 mM	0.1151 mL	0.5757 mL	1.1515 mL	2.8787 mL
	15 mM	0.0768 mL	0.3838 mL	0.7677 mL	1.9192 mL
	20 mM	0.0576 mL	0.2879 mL	0.5757 mL	1.4394 mL
	25 mM	0.0461 mL	0.2303 mL	0.4606 mL	1.1515 mL
	30 mM	0.0384 mL	0.1919 mL	0.3838 mL	0.9596 mL
	40 mM	0.0288 mL	0.1439 mL	0.2879 mL	0.7197 mL
	50 mM	0.0230 mL	0.1151 mL	0.2303 mL	0.5757 mL
	60 mM	0.0192 mL	0.0960 mL	0.1919 mL	0.4798 mL
	80 mM	0.0144 mL	0.0720 mL	0.1439 mL	0.3598 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Venetoclax (Synonyms: 维奈妥拉; ABT-199; GDC-0199; RG7601)

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Other Forms of Venetoclax:

MCE 顾客使用本产品发表的 208 篇科研文献

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Venetoclax (Synonyms: 维奈妥拉; ABT-199; GDC-0199; RG7601)

Customer Review

Other Forms of Venetoclax:

Venetoclax 相关抗体

MCE 顾客使用本产品发表的 208 篇科研文献

Venetoclax 相关产品

•相关化合物库:

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•同靶点蛋白产品:

查看 Bcl-2 Family 亚型特异性产品:

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参考文献

Venetoclax 相关抗体:

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