2. GPCR/G Protein Metabolic Enzyme/Protease
  3. Adenosine Receptor Endogenous Metabolite
  4. Theobromine

Theobromine  (Synonyms: 可可碱; 3,7-Dimethylxanthine)

目录号: HY-N0138 纯度: 99.78%
COA 产品使用指南 技术支持

Theobromine 是在可可豆中发现的甲基黄嘌呤，可以抑制腺苷受体 A1 (AR1) 信号传导。

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Theobromine

Theobromine Chemical Structure

CAS No. : 83-67-0

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥176
In-stock
10 mg ￥160
In-stock
25 mg ￥234
In-stock
50 mg ￥319
In-stock
100 mg ￥448
In-stock
500 mg ￥1049
In-stock
1 g ￥1538
In-stock
5 g   询价  
10 g   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Theobromine:

Theobromine 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 Adenosine Receptor 亚型特异性产品:

查看所有亚型
Adenosine A1 receptor (A1R) Adenosine A2A receptor (A2AR) Adenosine A2B receptor (A2BR) Adenosine A3 receptor (A3R)

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Theobromine is a methylxanthine found in cacao beans which can inhibit adenosine receptor A1 (AR1) signaling.

IC50 & Target

AR1[1]
体外研究
(In Vitro)

浓度高于 25 μM 的 Theobromine 会降低这些细胞中的脂质积累。细胞活力不受 Theobromine 影响。浓度高于 25 μM 的 Theobromine 会抑制 PPARγ、C/EBPα 和脂肪生成基因的蛋白质表达。这些基因的 mRNA 水平也会因可可碱而降低[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Theobromine 相关抗体:
体内研究
(In Vivo)

Theobromine 抑制附睾和肾周脂肪组织的增重。Theobromine 组的平均脂肪细胞面积小于载体组[1]。
以单位粪便重量的细菌数量计算，Theobromine 组的细菌数量低于其他组。Theobromine 饮食导致的 pH 值高于 RF 和 CC 饮食后的 pH 值。粪便乳酸浓度不受实验饮食的显著影响(RF 组为 4.26 mM；CC 组为 1.96 mM；可可碱组为 2.69 mM)[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

180.17

Formula

C7H8N4O2
CAS 号
性状

固体

颜色

White to off-white

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

DMSO 中的溶解度 : 1.1 mg/mL (6.11 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 5.5503 mL 27.7516 mL 55.5031 mL
5 mM 1.1101 mL 5.5503 mL 11.1006 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.78%

COA (291 KB) RP-HPLC (199 KB)

产品使用指南 (1538 KB)
参考文献
Kinase Assay
[1]

3T3-L1 preadipocytes are pre-incubated with MG132 (10 μM) for 30 min, followed by incubation with IBMX in the presence or absence of Theobromine (25 μM) for 8 h. The cells are lysed in denaturing cell extraction buffer (50 mM Tris-HCl, pH 7.5, containing 70 mM β-mercaptoethanol and 2% SDS) at 95°C for 10 min. The cell lysates are diluted 20 fold with dilution buffer (20 mM Tris-HCl, pH 7.5, containing 150 mM NaCl, 1 mM EDTA, 1mM EGTA, 1% TritonX-100, 2.5 mM sodium pyrophosphate and protease inhibitor cocktail) and centrifuged at 20,000 g for 30 s. The supernatant is incubated with rabbit polyclonal anti-C/EBPβ IgG, anti-FLAG IgG or control IgG at 4°C overnight, followed by incubation with 30 μL protein G-Sepharose resin at 4°C for 1 h. The resin is washed with lysis buffer three times and proteins bound to the resin are separated by SDS-PAGE and analyzed by western blotting[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Lewis rats (3 week old) are used in this study. The rats are randomly distributed into three dietary groups (n=7 per group): the reference (RF) group ingested standard diet AIN-93M, the cocoa (CC) group ingested a standard diet with 10% of natural Forastero cocoa containing 0.25% Theobromine, and the Theobromine (TB) group ingested a standard diet including 0.25% of Theobromine, i.e. the content of Theobromine presents in the CC diet[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Theobromine 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.5503 mL 27.7516 mL 55.5031 mL 138.7578 mL
5 mM 1.1101 mL 5.5503 mL 11.1006 mL 27.7516 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Theobromine83-67-03,7-DimethylxanthineAdenosine ReceptorEndogenous MetaboliteP1 receptorInhibitorinhibitorinhibit

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