1. Cell Cycle/DNA Damage Anti-infection
  2. Nucleoside Antimetabolite/Analog Orthopoxvirus
  3. Tiazofurin

Tiazofurin  (Synonyms: 噻唑呋林; NSC 286193; Riboxamide)

目录号: HY-114570 纯度: 99.84%
COA 产品使用指南 技术支持

Tiazofurin (NSC 286193) 是一种具有抗肿瘤活性的合成核苷类似物。Tiazofurin 在细胞内被代谢为噻唑-4-羧酰胺腺苷二核苷酸 (TAD),这是一种强效的 IMP 脱氢酶 (IMPDH) 抑制剂。Tiazofurin 增强 K562 细胞中 IL-6 的自体分泌。Tiazofurin 还具有抗正痘病毒 (orthopoxvirus) 和抗天花病毒活性。Tiazofurin 可用于白血病和肺癌研究

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Tiazofurin

Tiazofurin Chemical Structure

CAS No. : 60084-10-8

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Customer Review

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tiazofurin (NSC 286193) is a synthetic nucleoside analogue with antineoplastic activity. Tiazofurin is anabolized intracellularly to tiazole-4-carboxamide adenine dinucleotide (TAD), a potent inhibitor of IMP dehydrogenase (IMPDH). Tiazofurin enhances the autosecretion of IL-6 in K562 cells. Tiazofurin also has anti-orthopoxvirus and anti-variola activities. Tiazofurin can be used for the study of leukemia and lung cancer[1][2][3][4].

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
5637 IC50
24 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against 5637, human bladder carcinoma cell line
Compound was tested for antiproliferative activity against 5637, human bladder carcinoma cell line
[PMID: 9171883]
ACHN IC50
> 100 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against ACHN, human renal adenocarcinoma
Compound was tested for antiproliferative activity against ACHN, human renal adenocarcinoma
[PMID: 9171883]
B16 IC50
16.2 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against B16 melanoma cell lines
Inhibitory concentration against B16 melanoma cell lines
[PMID: 7562914]
B16 IC50
16.2 μM
Compound: tiazofurin
In vitro inhibitory activity against B16 murine melanoma cells
In vitro inhibitory activity against B16 murine melanoma cells
[PMID: 2120442]
CCRF-CEM IC50
10 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against CCRF-CEM, human acute T-lymphoblastic leukemia
Compound was tested for antiproliferative activity against CCRF-CEM, human acute T-lymphoblastic leukemia
[PMID: 9171883]
CCRF-SB IC50
7.5 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against CCRF-SB, human acute B-lymphoblastic leukemia
Compound was tested for antiproliferative activity against CCRF-SB, human acute B-lymphoblastic leukemia
[PMID: 9171883]
CHO-K1 IC50
6.4 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against CHO-K1, Chinese hamster ovary cells
Compound was tested for antiproliferative activity against CHO-K1, Chinese hamster ovary cells
[PMID: 9171883]
HL-60 IC50
0.19 μM
Compound: 1
Antiproliferative activity against human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
HL-60 IC50
0.19 μM
Compound: 1, Tiazofurin
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
[PMID: 23010263]
HL-60 IC50
0.19 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay
[PMID: 26859071]
HL-60 IC50
1.84 μM
Compound: 1, tiazofurin
Cytotoxicity against human HL60 cell line after 48 hrs by MTT assay
Cytotoxicity against human HL60 cell line after 48 hrs by MTT assay
[PMID: 17543526]
HL-60 IC50
2.5 μM
Compound: 1 (tiazofurin)
Inhibitory concentration range against human HL-60 cell line
Inhibitory concentration range against human HL-60 cell line
[PMID: 7562914]
HL-60 IC50
6.1 μM
Compound: tiazofurin
In vitro inhibitory activity against HL-60 human promyelocytic leukemia cells
In vitro inhibitory activity against HL-60 human promyelocytic leukemia cells
[PMID: 2120442]
HL-60 IC50
8.7 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against HL-60 human promyelocytic leukemia
Inhibitory concentration against HL-60 human promyelocytic leukemia
[PMID: 7562914]
HL-60 IC50
9.32 μM
Compound: 1
Antiproliferative activity against human HL60 cell line
Antiproliferative activity against human HL60 cell line
[PMID: 16495053]
HL-60 IC50
9.32 μM
Compound: 1 (Tiazofurin)
Concentration required to inhibit HL-60 cell growth
Concentration required to inhibit HL-60 cell growth
[PMID: 12951086]
HT-29 IC50
0.26 μM
Compound: 1
Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human HT-29 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
HT-29 IC50
0.26 μM
Compound: 1, Tiazofurin
Cytotoxicity against human HT-29 cells
Cytotoxicity against human HT-29 cells
[PMID: 23010263]
HT-29 IC50
0.26 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay
[PMID: 26859071]
HT-29 IC50
0.89 μM
Compound: 1, tiazofurin
Cytotoxicity against human HT29 cell line after 48 hrs by MTT assay
Cytotoxicity against human HT29 cell line after 48 hrs by MTT assay
[PMID: 17543526]
HT-29 IC50
1.01 μM
Compound: 1
Antiproliferative activity against human HT29 cell line
Antiproliferative activity against human HT29 cell line
[PMID: 16495053]
HT-29 IC50
1.01 μM
Compound: 1 (Tiazofurin)
Concentration required to inhibit HT-29 cell growth
Concentration required to inhibit HT-29 cell growth
[PMID: 12951086]
HT-29 IC50
1.01 μM
Compound: 1, NSC-286193, Tiazofurin
Cytotoxicity against HT29 cells after 24 hrs by MTT assay
Cytotoxicity against HT29 cells after 24 hrs by MTT assay
[PMID: 16908146]
HT-29 IC50
100 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against HT29, human colon adenocarcinoma
Compound was tested for antiproliferative activity against HT29, human colon adenocarcinoma
[PMID: 9171883]
HeLa IC50
100 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against HeLa, human cervix carcinoma
Compound was tested for antiproliferative activity against HeLa, human cervix carcinoma
[PMID: 9171883]
HeLa IC50
3.82 μM
Compound: 1
Antiproliferative activity against human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human HeLa cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
HeLa IC50
3.82 μM
Compound: 1, Tiazofurin
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
[PMID: 23010263]
HeLa IC50
3.82 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay
[PMID: 26859071]
HeLa IC50
4.76 μM
Compound: 1
Antiproliferative activity against human HeLa cell line
Antiproliferative activity against human HeLa cell line
[PMID: 16495053]
HeLa IC50
4.76 μM
Compound: 1, NSC-286193, Tiazofurin
Cytotoxicity against HeLa cells after 24 hrs by MTT assay
Cytotoxicity against HeLa cells after 24 hrs by MTT assay
[PMID: 16908146]
Jurkat IC50
0.04 μM
Compound: 1
Antiproliferative activity against human Jurkat cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human Jurkat cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
Jurkat IC50
0.04 μM
Compound: 1, Tiazofurin
Cytotoxicity against human Jurkat cells after 72 hrs by MTT assay
Cytotoxicity against human Jurkat cells after 72 hrs by MTT assay
[PMID: 23010263]
Jurkat IC50
0.04 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human Jurkat cells after 72 hrs by MTT assay
Antiproliferative activity against human Jurkat cells after 72 hrs by MTT assay
[PMID: 26859071]
Jurkat IC50
0.14 μM
Compound: 1, NSC-286193, Tiazofurin
Cytotoxicity against Jurkat cells after 24 hrs by MTT assay
Cytotoxicity against Jurkat cells after 24 hrs by MTT assay
[PMID: 16908146]
Jurkat IC50
0.51 μM
Compound: 1, tiazofurin
Cytotoxicity against human Jurkat T cells after 48 hrs by MTT assay
Cytotoxicity against human Jurkat T cells after 48 hrs by MTT assay
[PMID: 17543526]
K562 IC50
1.89 μM
Compound: 1
Antiproliferative activity against human K562 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human K562 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
K562 IC50
1.89 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
Antiproliferative activity against human K562 cells after 72 hrs by MTT assay
[PMID: 26859071]
K562 IC50
2.09 μM
Compound: 1, Tiazofurin
Cytotoxicity against human K562 cells after 72 hrs by MTT assay
Cytotoxicity against human K562 cells after 72 hrs by MTT assay
[PMID: 23010263]
K562 IC50
2.5 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against K562, human erythroid leukemia
Compound was tested for antiproliferative activity against K562, human erythroid leukemia
[PMID: 9171883]
K562 IC50
2.98 μM
Compound: 1, tiazofurin
Cytotoxicity against human K562 cells after 48 hrs by MTT assay
Cytotoxicity against human K562 cells after 48 hrs by MTT assay
[PMID: 17543526]
K562 IC50
3 μM
Compound: 1, TR
Concentration of the Antiproliferative compound required for the 50% inhibition of Growth against K562 cells
Concentration of the Antiproliferative compound required for the 50% inhibition of Growth against K562 cells
[PMID: 11806722]
K562 IC50
3 μM
Compound: TF (1)
Antiproliferative activity against K562 erythroid leukemic cells was determined.
Antiproliferative activity against K562 erythroid leukemic cells was determined.
[PMID: 9258359]
K562 IC50
3 μM
Compound: TR
Tested for inhibition of growth in human erythroleukemia K-562 cells.
Tested for inhibition of growth in human erythroleukemia K-562 cells.
[PMID: 9111303]
K562 IC50
4.16 μM
Compound: 1 (Tiazofurin)
Concentration required to inhibit K562 cell growth
Concentration required to inhibit K562 cell growth
[PMID: 12951086]
K562 IC50
4.5 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against K562 human myelogenous leukemia cell line
Inhibitory concentration against K562 human myelogenous leukemia cell line
[PMID: 7562914]
K562 IC50
5.29 μM
Compound: 1
Antiproliferative activity against human K562 cell line
Antiproliferative activity against human K562 cell line
[PMID: 16495053]
K562 IC50
5.29 μM
Compound: 1, NSC-286193, Tiazofurin
Cytotoxicity against human K562 cells after 24 hrs by MTT assay
Cytotoxicity against human K562 cells after 24 hrs by MTT assay
[PMID: 16908146]
L1210 IC50
2.9 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against L1210, murine lymphocytic leukemia
Compound was tested for antiproliferative activity against L1210, murine lymphocytic leukemia
[PMID: 9171883]
L1210 IC50
6.2 μM
Compound: tiazofurin
In vitro inhibitory activity against L1210 murine lymphocytic leukemia cells
In vitro inhibitory activity against L1210 murine lymphocytic leukemia cells
[PMID: 2120442]
L1210 IC50
7.4 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against L1210 murine lymphocytic leukemia
Inhibitory concentration against L1210 murine lymphocytic leukemia
[PMID: 7562914]
LoVo IC50
> 128 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against LoVo human colon adenocarcinoma cell lines
Inhibitory concentration against LoVo human colon adenocarcinoma cell lines
[PMID: 7562914]
MCF7 IC50
0.26 μM
Compound: 1, Tiazofurin
Cytotoxicity against human MCF7 cells
Cytotoxicity against human MCF7 cells
[PMID: 23010263]
MCF7 IC50
1.78 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay
[PMID: 26859071]
MCF7 IC50
6.39 μM
Compound: 1, tiazofurin
Cytotoxicity against human MCF7 cell line after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cell line after 48 hrs by MTT assay
[PMID: 17543526]
MCF7 IC50
7.98 μM
Compound: 1
Antiproliferative activity against human MCF7 cell line
Antiproliferative activity against human MCF7 cell line
[PMID: 16495053]
MCF7 IC50
8.49 μM
Compound: 1 (Tiazofurin)
Concentration required to inhibit MCF-7 cell growth
Concentration required to inhibit MCF-7 cell growth
[PMID: 12951086]
MDA-MB-231 IC50
0.26 μM
Compound: 1, Tiazofurin
Cytotoxicity against human MDA-MB-231 cells
Cytotoxicity against human MDA-MB-231 cells
[PMID: 23010263]
MOLT-4 IC50
5.2 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against MOLT-4, human acute T-lymphoblastic leukemia
Compound was tested for antiproliferative activity against MOLT-4, human acute T-lymphoblastic leukemia
[PMID: 9171883]
MRC5 IC50
0.36 μM
Compound: 1
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 31557614]
MRC5 IC50
0.36 μM
Compound: 1, Tiazofurin
Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay
Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay
[PMID: 23010263]
MRC5 IC50
0.36 μM
Compound: 1; Tiazofurin
Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay
Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay
[PMID: 26859071]
MRC5 IC50
0.49 μM
Compound: 1, tiazofurin
Cytotoxicity against human MRC5 cell line after 48 hrs by MTT assay
Cytotoxicity against human MRC5 cell line after 48 hrs by MTT assay
[PMID: 17543526]
MRC5 IC50
0.85 μM
Compound: 1
Antiproliferative activity against human MRC5 cell line
Antiproliferative activity against human MRC5 cell line
[PMID: 16495053]
MRC5 IC50
0.85 μM
Compound: 1 (Tiazofurin)
Concentration required to inhibit MRC-5 cell growth
Concentration required to inhibit MRC-5 cell growth
[PMID: 12951086]
MRC5 IC50
0.85 μM
Compound: 1, NSC-286193, Tiazofurin
Cytotoxicity against MRC5 cells after 24 hrs by MTT assay
Cytotoxicity against MRC5 cells after 24 hrs by MTT assay
[PMID: 16908146]
P388 IC50
16.7 μM
Compound: tiazofurin
In vitro inhibitory activity against P388 murine lymphocytic leukemia cells
In vitro inhibitory activity against P388 murine lymphocytic leukemia cells
[PMID: 2120442]
P388 IC50
2.2 μM
Compound: 1 (tiazofurin)
Inhibitory concentration against P388 murine lymphocytic leukemia cell lines
Inhibitory concentration against P388 murine lymphocytic leukemia cell lines
[PMID: 7562914]
Raji IC50
16.06 μM
Compound: 1, tiazofurin
Cytotoxicity against human Raji cells after 48 hrs by MTT assay
Cytotoxicity against human Raji cells after 48 hrs by MTT assay
[PMID: 17543526]
Raji IC50
5.28 μM
Compound: 1
Antiproliferative activity against human Raji cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
Antiproliferative activity against human Raji cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay
[PMID: 31557614]
Raji IC50
5.28 μM
Compound: 1, Tiazofurin
Cytotoxicity against human Raji cells after 72 hrs by MTT assay
Cytotoxicity against human Raji cells after 72 hrs by MTT assay
[PMID: 23010263]
Raji IC50
5.28 μM
Compound: 1; Tiazofurin
Antiproliferative activity against human Raji cells after 72 hrs by MTT assay
Antiproliferative activity against human Raji cells after 72 hrs by MTT assay
[PMID: 26859071]
Raji IC50
8.2 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against Raji, human Burkitt lymphoma
Compound was tested for antiproliferative activity against Raji, human Burkitt lymphoma
[PMID: 9171883]
WIL2-NS IC50
5.7 μM
Compound: Tiazofurin
Compound was tested for antiproliferative activity against Wil2-NS, human splenic lymphoblastoid cells
Compound was tested for antiproliferative activity against Wil2-NS, human splenic lymphoblastoid cells
[PMID: 9171883]
体外研究
(In Vitro)

Tiazofurin (10 μM, 0-3 天) 影响 K562 人白血病细胞的生长动力学并促进红系分化,同时显著增加苯胺啶阳性细胞的百分比并提高糖蛋白 A (GpA) 的表达[1]
Tiazofurin (10 μM, 2 天) 增强 K562 细胞中 IL-6 的自体分泌[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Tiazofurin (200-700 mg/kg,腹腔注射,每日一次,第 1-9 天) 在敏感的小鼠移植肿瘤模型 (CDF1 小鼠中的 P388/L1210 白血病,B6C3F1 小鼠中的 Lewis 肺癌) 中发挥显著的抗肿瘤疗效[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: leukemia cells (P388/L1210) and Lewis lung carcinoma cells were intravenously inoculated into B6C3F1 mice or CDF1 mice[2]
Dosage: 200, 700 mg/kg
Administration: i.p., qd days 1-9
Result: Exerted significant anti-tumor efficacy in sensitive murine transplanted tumor models (P388/L1210 leukemia in CDF1 mice, Lewis lung carcinoma in B6C3F1 mice).
分子量

260.27

Formula

C9H12N2O5S

CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验: 

DMSO 中的溶解度 : 125 mg/mL (480.27 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.8422 mL 19.2108 mL 38.4216 mL
5 mM 0.7684 mL 3.8422 mL 7.6843 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.8422 mL 19.2108 mL 38.4216 mL 96.0541 mL
5 mM 0.7684 mL 3.8422 mL 7.6843 mL 19.2108 mL
10 mM 0.3842 mL 1.9211 mL 3.8422 mL 9.6054 mL
15 mM 0.2561 mL 1.2807 mL 2.5614 mL 6.4036 mL
20 mM 0.1921 mL 0.9605 mL 1.9211 mL 4.8027 mL
25 mM 0.1537 mL 0.7684 mL 1.5369 mL 3.8422 mL
30 mM 0.1281 mL 0.6404 mL 1.2807 mL 3.2018 mL
40 mM 0.0961 mL 0.4803 mL 0.9605 mL 2.4014 mL
50 mM 0.0768 mL 0.3842 mL 0.7684 mL 1.9211 mL
60 mM 0.0640 mL 0.3202 mL 0.6404 mL 1.6009 mL
80 mM 0.0480 mL 0.2401 mL 0.4803 mL 1.2007 mL
100 mM 0.0384 mL 0.1921 mL 0.3842 mL 0.9605 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Tiazofurin
目录号:
HY-114570
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