Romidepsin (Synonyms: 罗米地辛; FK 228; FR 901228; NSC 630176)

目录号: HY-15149 纯度: 99.90%: Data Sheet SDS COA 产品使用指南
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技术支持

Romidepsin (FK 228) 是具有抗肿瘤活性的组蛋白去乙酰化酶 (HDAC) 抑制剂，抑制 HDAC1，HDAC2，HDAC4 和 HDAC6，IC₅₀ 值分别为 36 nM，47 nM，510 nM 和 1.4 μM。Romidepsin (FK 228) 由紫色杆菌产生，诱导 G2/M 细胞周期阻滞和凋亡 (apoptosis)。

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Romidepsin Chemical Structure

CAS No. : 128517-07-7

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Customer Review

Other Forms of Romidepsin:

Romidepsin-d₇ 询价
Romidepsin (GMP) 询价

MCE 顾客使用本产品发表的 65 篇科研文献

Cell Viability Assay

: Romidepsin purchased from MCE. Usage Cited in: Cancer Cell. 2023 Mar 13;41(3):602-619.e11. [Abstract]; The triple combination of E + R + iC significantly decreases tumor volume compared with double combinations, in mice. E: enzalutamide (30 mg/kg; p.o.; single daily for 10 days); R: Romidepsin (0.3 mg/kg; i.p.; single daily for 10 days); iC: CXCR2 inhibitor.

: Romidepsin purchased from MCE. Usage Cited in: PLoS Pathog. 2018 Sep 13;14(9):e1007267. [Abstract]; Immunoblot analysis for vIL-6, ORF45, or actin is performed on lysates from BCBL-1 cells treated with NaB+TPA, Romidepsin or LBH589.

: Romidepsin purchased from MCE. Usage Cited in: Neurosci Lett. 2017 Jul 13:653:12-18. [Abstract]; Romidepsin increases histone acetylation in SH-SY5Y neuroblastoma cells. Western blots of pAcH3 and β-actin levels in SH-SY5Y cells cultured for 24 h with 0-40 nM of Romidepsin. 20 nM and 40 nM Romidepsin significantly increase the levels of pAcH3 relative to control, which is measured by densitometric quantification of pAcH3 protein levels relative to β-actin loading control.

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HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

生物活性
纯度 & 产品资料
参考文献

生物活性

Romidepsin (FK 228) is a Histone deacetylase (HDAC) inhibitor with anti-tumor activities. Romidepsin (FK 228) inhibits HDAC1, HDAC2, HDAC4, and HDAC6 with IC₅₀s of 36 nM, 47 nM, 510 nM and 1.4 μM, respectively^[1]. Romidepsin (FK 228) is produced by Chromobacterium violaceum, induces cell G2/M phase arrest and apoptosis^[2].

IC₅₀ & Target^[1]

HDAC1

36 nM (IC₅₀)

HDAC2

47 nM (IC₅₀)

HDAC4

510 nM (IC₅₀)

HDAC6

14000 nM (IC₅₀)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	GI₅₀	2.6 nM Compound: 1, FK228	Growth inhibition of human A549 cells by sulforhodamine B colorimetric method Growth inhibition of human A549 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
A549	GI₅₀	2.6 nM Compound: 1; FK228	Growth inhibition of human A549 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human A549 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
A549	GI₅₀	2.6 nM Compound: 5, FK228	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 24589486]
A549	IC₅₀	0.025 μM Compound: FK228	Growth inhibition of human A549 cells Growth inhibition of human A549 cells	[PMID: 28814374]
ACHN	GI₅₀	20 nM Compound: 1, FK228	Growth inhibition of human ACHN cells by sulforhodamine B colorimetric method Growth inhibition of human ACHN cells by sulforhodamine B colorimetric method	[PMID: 23313638]
ACHN	GI₅₀	20 nM Compound: 1; FK228	Growth inhibition of human ACHN cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human ACHN cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
ACHN	GI₅₀	20 nM Compound: 5, FK228	Cytotoxicity against human ACHN cells by SRB assay Cytotoxicity against human ACHN cells by SRB assay	[PMID: 24589486]
DMS-114	GI₅₀	3.6 nM Compound: 1, FK228	Growth inhibition of human DMS114 cells by sulforhodamine B colorimetric method Growth inhibition of human DMS114 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
DMS-114	GI₅₀	3.6 nM Compound: 1; FK228	Growth inhibition of human DMS-114 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human DMS-114 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
DMS-114	GI₅₀	3.6 nM Compound: 5, FK228	Cytotoxicity against human DMS114 cells by SRB assay Cytotoxicity against human DMS114 cells by SRB assay	[PMID: 24589486]
DMS-273	GI₅₀	5.8 nM Compound: 1, FK228	Growth inhibition of human DMS273 cells by sulforhodamine B colorimetric method Growth inhibition of human DMS273 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
DMS-273	GI₅₀	5.8 nM Compound: 1; FK228	Growth inhibition of human DMS-273 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human DMS-273 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
DMS-273	GI₅₀	5.8 nM Compound: 5, FK228	Cytotoxicity against human DMS273 cells by SRB assay Cytotoxicity against human DMS273 cells by SRB assay	[PMID: 24589486]
DU-145	GI₅₀	2.55 nM Compound: 1, FK228	Antiproliferative activity against human DU145 cells after 72 hrs by CCK8 assay Antiproliferative activity against human DU145 cells after 72 hrs by CCK8 assay	[PMID: 26331334]
DU-145	GI₅₀	6 nM Compound: 1, FK228	Growth inhibition of human DU145 cells by sulforhodamine B colorimetric method Growth inhibition of human DU145 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
DU-145	GI₅₀	6 nM Compound: 1; FK228	Growth inhibition of human DU-145 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human DU-145 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
DU-145	GI₅₀	6 nM Compound: 5, FK228	Cytotoxicity against human DU145 cells by SRB assay Cytotoxicity against human DU145 cells by SRB assay	[PMID: 24589486]
HCC 2998	GI₅₀	3.1 nM Compound: 1, FK228	Growth inhibition of human HCC2998 cells by sulforhodamine B colorimetric method Growth inhibition of human HCC2998 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
HCC 2998	GI₅₀	3.1 nM Compound: 1; FK228	Growth inhibition of human HCC 2998 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human HCC 2998 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
HCC 2998	GI₅₀	3.1 nM Compound: 5, FK228	Cytotoxicity against human HCC2998 cells by SRB assay Cytotoxicity against human HCC2998 cells by SRB assay	[PMID: 24589486]
HCT-116	GI₅₀	11.6 nM Compound: FK228	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 31057733]
HCT-116	GI₅₀	3.1 nM Compound: 1, FK228	Growth inhibition of human HCT116 cells by sulforhodamine B colorimetric method Growth inhibition of human HCT116 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
HCT-116	GI₅₀	3.1 nM Compound: 1; FK228	Growth inhibition of human HCT-116 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT-116 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
HCT-116	GI₅₀	3.1 nM Compound: 5, FK228	Cytotoxicity against human HCT116 cells by SRB assay Cytotoxicity against human HCT116 cells by SRB assay	[PMID: 24589486]
HCT-116	IC₅₀	0.028 μM Compound: Romidepsin	Antiproliferative activity against human HCT116 cells assessed as cell viability incubated for 72 hrs by coulter counter method Antiproliferative activity against human HCT116 cells assessed as cell viability incubated for 72 hrs by coulter counter method	[PMID: 26481659]
HCT-116	IC₅₀	3 nM Compound: FK228	Antiproliferative activity against human HCT116 cells after 48 hrs in presence of DTT by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs in presence of DTT by MTT assay	[PMID: 25147612]
HCT-15	GI₅₀	450 nM Compound: 1, FK228	Growth inhibition of human HCT15 cells by sulforhodamine B colorimetric method Growth inhibition of human HCT15 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
HCT-15	GI₅₀	450 nM Compound: 1; FK228	Growth inhibition of human HCT-15 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human HCT-15 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
HCT-15	GI₅₀	450 nM Compound: 2; FK228	Cytotoxicity against human HCT-15 cells assessed as cell growth inhibition by sulforhodamine B colorimetric method Cytotoxicity against human HCT-15 cells assessed as cell growth inhibition by sulforhodamine B colorimetric method	[PMID: 34826681]
HCT-15	GI₅₀	450 nM Compound: 5, FK228	Cytotoxicity against human HCT15 cells by SRB assay Cytotoxicity against human HCT15 cells by SRB assay	[PMID: 24589486]
HEK293	CC₅₀	0.004 μM Compound: Romidepsin	Cytotoxicity against HEK293 cells assessed as reduction in cell growth incubated for 48 hrs by MTS assay Cytotoxicity against HEK293 cells assessed as reduction in cell growth incubated for 48 hrs by MTS assay	[PMID: 30973727]
HEK293	IC₅₀	<= 0.001 μM Compound: 11; FK228	Cytotoxicity against HEK293 cells after 48 hrs by resazurin assay Cytotoxicity against HEK293 cells after 48 hrs by resazurin assay	[PMID: 28241112]
HEK293	IC₅₀	<= 1 nM Compound: Romidepsin	Cytotoxicity against HEK293 cells after 48 hrs by resazurin dye based assay Cytotoxicity against HEK293 cells after 48 hrs by resazurin dye based assay	[PMID: 30245402]
HT-29	GI₅₀	3.3 nM Compound: 1, FK228	Growth inhibition of human HT-29 cells by sulforhodamine B colorimetric method Growth inhibition of human HT-29 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
HT-29	GI₅₀	3.3 nM Compound: 1; FK228	Growth inhibition of human HT-29 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human HT-29 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
HT-29	GI₅₀	3.3 nM Compound: 5, FK228	Cytotoxicity against human HT-29 cells by SRB assay Cytotoxicity against human HT-29 cells by SRB assay	[PMID: 24589486]
HepG2	CC₅₀	0.01 μM Compound: Romidepsin	Cytotoxicity against human HepG2 cells assessed as reduction in cell growth incubated for 48 hrs by CellTiter-Glo assay Cytotoxicity against human HepG2 cells assessed as reduction in cell growth incubated for 48 hrs by CellTiter-Glo assay	[PMID: 30973727]
HepG2	IC₅₀	2.4 ng/mL Compound: FK228; 2	Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 3 days by trypan blue assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 3 days by trypan blue assay	[PMID: 35586423]
IGROV-1	IC₅₀	0.08 μM Compound: Romidepsin	Antiproliferative activity against human IGROV1/Pt1 cells assessed as cell viability incubated for 72 hrs by coulter counter method Antiproliferative activity against human IGROV1/Pt1 cells assessed as cell viability incubated for 72 hrs by coulter counter method	[PMID: 26481659]
IGROV-1	IC₅₀	0.22 μM Compound: Romidepsin	Antiproliferative activity against human IGROV1 cells assessed as cell viability incubated for 72 hrs by coulter counter method Antiproliferative activity against human IGROV1 cells assessed as cell viability incubated for 72 hrs by coulter counter method	[PMID: 26481659]
KM12	GI₅₀	3.4 nM Compound: 1, FK228	Growth inhibition of human KM12 cells by sulforhodamine B colorimetric method Growth inhibition of human KM12 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
KM12	GI₅₀	3.4 nM Compound: 1; FK228	Growth inhibition of human KM12 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human KM12 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
KM12	GI₅₀	3.4 nM Compound: 5, FK228	Cytotoxicity against human KM12 cells by SRB assay Cytotoxicity against human KM12 cells by SRB assay	[PMID: 24589486]
LOX IMVI	GI₅₀	2.5 nM Compound: 1, FK228	Growth inhibition of human LOXIMVI cells by sulforhodamine B colorimetric method Growth inhibition of human LOXIMVI cells by sulforhodamine B colorimetric method	[PMID: 23313638]
LOX IMVI	GI₅₀	2.5 nM Compound: 1; FK228	Growth inhibition of human LOX IMVI cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human LOX IMVI cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
LOX IMVI	GI₅₀	2.5 nM Compound: 5, FK228	Cytotoxicity against human LOXIMVI cells by SRB assay Cytotoxicity against human LOXIMVI cells by SRB assay	[PMID: 24589486]
MCF7	GI₅₀	4.2 nM Compound: 1, FK228	Growth inhibition of human MCF7 cells by sulforhodamine B colorimetric method Growth inhibition of human MCF7 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MCF7	GI₅₀	4.2 nM Compound: 1; FK228	Growth inhibition of human MCF7 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MCF7 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MCF7	GI₅₀	4.2 nM Compound: 5, FK228	Cytotoxicity against human MCF7 cells by SRB assay Cytotoxicity against human MCF7 cells by SRB assay	[PMID: 24589486]
MCF7	IC₅₀	0.75 nM Compound: FK-228	Growth inhibition of MCF7 cells Growth inhibition of MCF7 cells	[PMID: 17958342]
MDA-MB-231	GI₅₀	11.5 nM Compound: FK228	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 31057733]
MDA-MB-231	GI₅₀	5.5 nM Compound: 1, FK228	Growth inhibition of human MDA-MB-231 cells by sulforhodamine B colorimetric method Growth inhibition of human MDA-MB-231 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MDA-MB-231	GI₅₀	5.5 nM Compound: 1; FK228	Growth inhibition of human MDA-MB-231 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MDA-MB-231 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MDA-MB-231	GI₅₀	5.5 nM Compound: 5, FK228	Cytotoxicity against human MDA-MB-231 cells by SRB assay Cytotoxicity against human MDA-MB-231 cells by SRB assay	[PMID: 24589486]
MDA-MB-231	GI₅₀	9.7 nM Compound: 2	Antiproliferative activity against triple-negative human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against triple-negative human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 33745280]
MKN-1	GI₅₀	3.2 nM Compound: 1, FK228	Growth inhibition of human MKN1 cells by sulforhodamine B colorimetric method Growth inhibition of human MKN1 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MKN-1	GI₅₀	3.2 nM Compound: 1; FK228	Growth inhibition of human MKN-1 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MKN-1 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MKN-1	GI₅₀	3.2 nM Compound: 5, FK228	Cytotoxicity against human MKN1 cells by SRB assay Cytotoxicity against human MKN1 cells by SRB assay	[PMID: 24589486]
MKN-28	GI₅₀	17 nM Compound: 1, FK228	Growth inhibition of human MKN28 cells by sulforhodamine B colorimetric method Growth inhibition of human MKN28 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MKN-28	GI₅₀	17 nM Compound: 1; FK228	Growth inhibition of human MKN-28 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MKN-28 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MKN-28	GI₅₀	17 nM Compound: 5, FK228	Cytotoxicity against human MKN28 cells by SRB assay Cytotoxicity against human MKN28 cells by SRB assay	[PMID: 24589486]
MKN-45	GI₅₀	14 nM Compound: 1, FK228	Growth inhibition of human MKN45 cells by sulforhodamine B colorimetric method Growth inhibition of human MKN45 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MKN-45	GI₅₀	14 nM Compound: 1; FK228	Growth inhibition of human MKN-45 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MKN-45 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MKN-45	GI₅₀	14 nM Compound: 5, FK228	Cytotoxicity against human MKN45 cells by SRB assay Cytotoxicity against human MKN45 cells by SRB assay	[PMID: 24589486]
MKN-7	GI₅₀	4.9 nM Compound: 1, FK228	Growth inhibition of human MKN7 cells by sulforhodamine B colorimetric method Growth inhibition of human MKN7 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MKN-7	GI₅₀	4.9 nM Compound: 1; FK228	Growth inhibition of human MKN-7 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MKN-7 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MKN-7	GI₅₀	4.9 nM Compound: 5, FK228	Cytotoxicity against human MKN7 cells by SRB assay Cytotoxicity against human MKN7 cells by SRB assay	[PMID: 24589486]
MKN-74	GI₅₀	3 nM Compound: 1, FK228	Growth inhibition of human MKN74 cells by sulforhodamine B colorimetric method Growth inhibition of human MKN74 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
MKN-74	GI₅₀	3 nM Compound: 1; FK228	Growth inhibition of human MKN-74 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MKN-74 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
MKN-74	GI₅₀	3 nM Compound: 5, FK228	Cytotoxicity against human MKN74 cells by SRB assay Cytotoxicity against human MKN74 cells by SRB assay	[PMID: 24589486]
MOLT-4	GI₅₀	2.06 nM Compound: 1, FK228	Antiproliferative activity against human MOLT4 cells after 72 hrs by CCK8 assay Antiproliferative activity against human MOLT4 cells after 72 hrs by CCK8 assay	[PMID: 26331334]
NCI-H226	GI₅₀	8.9 nM Compound: 1, FK228	Growth inhibition of human NCI-H226 cells by sulforhodamine B colorimetric method Growth inhibition of human NCI-H226 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
NCI-H226	GI₅₀	8.9 nM Compound: 1; FK228	Growth inhibition of human NCI-H226 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H226 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
NCI-H226	GI₅₀	8.9 nM Compound: 5, FK228	Cytotoxicity against human NCI-H226 cells by SRB assay Cytotoxicity against human NCI-H226 cells by SRB assay	[PMID: 24589486]
NCI-H23	GI₅₀	4.6 nM Compound: 1, FK228	Growth inhibition of human NCI-H23 cells by sulforhodamine B colorimetric method Growth inhibition of human NCI-H23 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
NCI-H23	GI₅₀	4.6 nM Compound: 1; FK228	Growth inhibition of human NCI-H23 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H23 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
NCI-H23	GI₅₀	4.6 nM Compound: 5, FK228	Cytotoxicity against human NCI-H23 cells by SRB assay Cytotoxicity against human NCI-H23 cells by SRB assay	[PMID: 24589486]
NCI-H460	GI₅₀	3 nM Compound: 1, FK228	Growth inhibition of human NCI-H460 cells by sulforhodamine B colorimetric method Growth inhibition of human NCI-H460 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
NCI-H460	GI₅₀	3 nM Compound: 1; FK228	Growth inhibition of human NCI-H460 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H460 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
NCI-H460	GI₅₀	3 nM Compound: 5, FK228	Cytotoxicity against human NCI-H460 cells by SRB assay Cytotoxicity against human NCI-H460 cells by SRB assay	[PMID: 24589486]
NCI-H522	GI₅₀	1.8 nM Compound: 1, FK228	Growth inhibition of human NCI-H522 cells by sulforhodamine B colorimetric method Growth inhibition of human NCI-H522 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
NCI-H522	GI₅₀	1.8 nM Compound: 1; FK228	Growth inhibition of human NCI-H522 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H522 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
NCI-H522	GI₅₀	1.8 nM Compound: 2; FK228	Cytotoxicity against human NCI-H522 cells assessed as cell growth inhibition by sulforhodamine B colorimetric method Cytotoxicity against human NCI-H522 cells assessed as cell growth inhibition by sulforhodamine B colorimetric method	[PMID: 34826681]
NCI-H522	GI₅₀	1.8 nM Compound: 5, FK228	Cytotoxicity against human NCI-H522 cells by SRB assay Cytotoxicity against human NCI-H522 cells by SRB assay	[PMID: 24589486]
NFF	IC₅₀	0.001 μM Compound: Romidepsin	Cytotoxicity against human NFF cells incubated for 72 hrs by SRB assay Cytotoxicity against human NFF cells incubated for 72 hrs by SRB assay	[PMID: 39208744]
NFF	IC₅₀	<= 0.001 μM Compound: 11; FK228	Cytotoxicity against human NFF cells after 72 hrs by SRB assay Cytotoxicity against human NFF cells after 72 hrs by SRB assay	[PMID: 28241112]
NFF	IC₅₀	<= 1 nM Compound: Romidepsin	Cytotoxicity against human NFF cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human NFF cells after 72 hrs by sulforhodamine B assay	[PMID: 30245402]
OVCAR-3	GI₅₀	4.6 nM Compound: 1, FK228	Growth inhibition of human OVCAR3 cells by sulforhodamine B colorimetric method Growth inhibition of human OVCAR3 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
OVCAR-3	GI₅₀	4.6 nM Compound: 1; FK228	Growth inhibition of human OVCAR-3 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR-3 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
OVCAR-3	GI₅₀	4.6 nM Compound: 5, FK228	Cytotoxicity against human OVCAR3 cells by SRB assay Cytotoxicity against human OVCAR3 cells by SRB assay	[PMID: 24589486]
OVCAR-4	GI₅₀	20 nM Compound: 1, FK228	Growth inhibition of human OVCAR4 cells by sulforhodamine B colorimetric method Growth inhibition of human OVCAR4 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
OVCAR-4	GI₅₀	20 nM Compound: 1; FK228	Growth inhibition of human OVCAR-4 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR-4 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
OVCAR-4	GI₅₀	20 nM Compound: 5, FK228	Cytotoxicity against human OVCAR4 cells by SRB assay Cytotoxicity against human OVCAR4 cells by SRB assay	[PMID: 24589486]
OVCAR-5	GI₅₀	2.8 nM Compound: 1, FK228	Growth inhibition of human OVCAR5 cells by sulforhodamine B colorimetric method Growth inhibition of human OVCAR5 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
OVCAR-5	GI₅₀	2.8 nM Compound: 1; FK228	Growth inhibition of human OVCAR-5 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR-5 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
OVCAR-5	GI₅₀	2.8 nM Compound: 5, FK228	Cytotoxicity against human OVCAR5 cells by SRB assay Cytotoxicity against human OVCAR5 cells by SRB assay	[PMID: 24589486]
OVCAR-8	GI₅₀	5.5 nM Compound: 1, FK228	Growth inhibition of human OVCAR8 cells by sulforhodamine B colorimetric method Growth inhibition of human OVCAR8 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
OVCAR-8	GI₅₀	5.5 nM Compound: 1; FK228	Growth inhibition of human OVCAR-8 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human OVCAR-8 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
OVCAR-8	GI₅₀	5.5 nM Compound: 5, FK228	Cytotoxicity against human OVCAR8 cells by SRB assay Cytotoxicity against human OVCAR8 cells by SRB assay	[PMID: 24589486]
PC-3	GI₅₀	18 nM Compound: 1, FK228	Growth inhibition of human PC3 cells by sulforhodamine B colorimetric method Growth inhibition of human PC3 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
PC-3	GI₅₀	18 nM Compound: 1; FK228	Growth inhibition of human PC-3 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human PC-3 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
PC-3	GI₅₀	18 nM Compound: 5, FK228	Cytotoxicity against human PC3 cells by SRB assay Cytotoxicity against human PC3 cells by SRB assay	[PMID: 24589486]
RXF 631	GI₅₀	6.6 nM Compound: 1, FK228	Growth inhibition of human RXF631L cells by sulforhodamine B colorimetric method Growth inhibition of human RXF631L cells by sulforhodamine B colorimetric method	[PMID: 23313638]
RXF 631	GI₅₀	6.6 nM Compound: 1; FK228	Growth inhibition of human RXF 631 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human RXF 631 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
RXF 631	GI₅₀	6.6 nM Compound: 5, FK228	Cytotoxicity against human RXF631L cells by SRB assay Cytotoxicity against human RXF631L cells by SRB assay	[PMID: 24589486]
SF-268	GI₅₀	4.9 nM Compound: 1, FK228	Growth inhibition of human SF268 cells by sulforhodamine B colorimetric method Growth inhibition of human SF268 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SF-268	GI₅₀	4.9 nM Compound: 1; FK228	Growth inhibition of human SF-268 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SF-268 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SF-268	GI₅₀	4.9 nM Compound: 5, FK228	Cytotoxicity against human SF268 cells by SRB assay Cytotoxicity against human SF268 cells by SRB assay	[PMID: 24589486]
SF-295	GI₅₀	4 nM Compound: 1, FK228	Growth inhibition of human SF295 cells by sulforhodamine B colorimetric method Growth inhibition of human SF295 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SF-295	GI₅₀	4 nM Compound: 1; FK228	Growth inhibition of human SF-295 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SF-295 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SF-295	GI₅₀	4 nM Compound: 5, FK228	Cytotoxicity against human SF295 cells by SRB assay Cytotoxicity against human SF295 cells by SRB assay	[PMID: 24589486]
SF-539	GI₅₀	3.6 nM Compound: 1, FK228	Growth inhibition of human SF539 cells by sulforhodamine B colorimetric method Growth inhibition of human SF539 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SF-539	GI₅₀	3.6 nM Compound: 1; FK228	Growth inhibition of human SF-539 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SF-539 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SF-539	GI₅₀	3.6 nM Compound: 5, FK228	Cytotoxicity against human SF539 cells by SRB assay Cytotoxicity against human SF539 cells by SRB assay	[PMID: 24589486]
SK-OV-3	GI₅₀	3.3 nM Compound: 1, FK228	Growth inhibition of human SKOV3 cells by sulforhodamine B colorimetric method Growth inhibition of human SKOV3 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SK-OV-3	GI₅₀	3.3 nM Compound: 1; FK228	Growth inhibition of human SK-OV-3 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SK-OV-3 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SK-OV-3	GI₅₀	3.3 nM Compound: 5, FK228	Cytotoxicity against human SKOV3 cells by SRB assay Cytotoxicity against human SKOV3 cells by SRB assay	[PMID: 24589486]
SNB-75	GI₅₀	7.2 nM Compound: 1, FK228	Growth inhibition of human SNB75 cells by sulforhodamine B colorimetric method Growth inhibition of human SNB75 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SNB-75	GI₅₀	7.2 nM Compound: 1; FK228	Growth inhibition of human SNB-75 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB-75 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SNB-75	GI₅₀	7.2 nM Compound: 5, FK228	Cytotoxicity against human SNB75 cells by SRB assay Cytotoxicity against human SNB75 cells by SRB assay	[PMID: 24589486]
SNB-78	GI₅₀	9.6 nM Compound: 1, FK228	Growth inhibition of human SNB78 cells by sulforhodamine B colorimetric method Growth inhibition of human SNB78 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
SNB-78	GI₅₀	9.6 nM Compound: 1; FK228	Growth inhibition of human SNB-78 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SNB-78 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
SNB-78	GI₅₀	9.6 nM Compound: 5, FK228	Cytotoxicity against human SNB78 cells by SRB assay Cytotoxicity against human SNB78 cells by SRB assay	[PMID: 24589486]
Sf9	IC₅₀	0.0024 μM Compound: FK228	Inhibition of human recombinant C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay Inhibition of human recombinant C-terminal FLAG/His-tagged HDAC1 expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay	[PMID: 31057733]
Sf9	IC₅₀	0.0091 μM Compound: FK228	Inhibition of human recombinant C-terminal FLAG-tagged HDAC6 expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay Inhibition of human recombinant C-terminal FLAG-tagged HDAC6 expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay	[PMID: 31057733]
Sf9	IC₅₀	4.462 μM Compound: FK228	Inhibition of human recombinant C-terminal GST-tagged HDAC4 (101 to 1084 residues) expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay Inhibition of human recombinant C-terminal GST-tagged HDAC4 (101 to 1084 residues) expressed in baculovirus infected Sf9 insect cells after 1 hr by HDAC-Glo1/2 luminescent assay	[PMID: 31057733]
St-4	GI₅₀	22 nM Compound: 1, FK228	Growth inhibition of human St-4 cells by sulforhodamine B colorimetric method Growth inhibition of human St-4 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
St-4	GI₅₀	22 nM Compound: 1; FK228	Growth inhibition of human St-4 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human St-4 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
St-4	GI₅₀	22 nM Compound: 5, FK228	Cytotoxicity against human St-4 cells by SRB assay Cytotoxicity against human St-4 cells by SRB assay	[PMID: 24589486]
T-cell	EC₅₀	0.003 μM Compound: Romidepsin, istodax	Reactivation of latent HIV1 NL4-3-Luc expression in human naive CD4+ T cells treated 7 days post-infection measured after 48 hrs by luminescence plate reader analysis Reactivation of latent HIV1 NL4-3-Luc expression in human naive CD4+ T cells treated 7 days post-infection measured after 48 hrs by luminescence plate reader analysis	[PMID: 24495105]
U-251	GI₅₀	3.9 nM Compound: 1, FK228	Growth inhibition of human U251 cells by sulforhodamine B colorimetric method Growth inhibition of human U251 cells by sulforhodamine B colorimetric method	[PMID: 23313638]
U-251	GI₅₀	3.9 nM Compound: 1; FK228	Growth inhibition of human U-251 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human U-251 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 27318982]
U-251	GI₅₀	3.9 nM Compound: 5, FK228	Cytotoxicity against human U251 cells by SRB assay Cytotoxicity against human U251 cells by SRB assay	[PMID: 24589486]
U-937	CC₅₀	0.73 nM Compound: Romidepsin	Cytotoxicity against human U937 cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo luminescent cell viability assay Cytotoxicity against human U937 cells assessed as reduction in cell viability after 48 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 29541372]
U-937	GI₅₀	3.41 nM Compound: 1, FK228	Antiproliferative activity against human U937 cells after 72 hrs by CCK8 assay Antiproliferative activity against human U937 cells after 72 hrs by CCK8 assay	[PMID: 26331334]
WI-38	GI₅₀	30.1 nM Compound: 2	Cytotoxicity against human WI-38 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Cytotoxicity against human WI-38 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 33745280]
WI-38	GI₅₀	5.39 nM Compound: 1, FK228	Antiproliferative activity against human WI38 cells after 72 hrs by CCK8 assay Antiproliferative activity against human WI38 cells after 72 hrs by CCK8 assay	[PMID: 26331334]

体外研究
(In Vitro)

Romidepsin (0-72 hours；0 -80 nM) 以剂量依赖性方式抑制 HCC 细胞增殖^[2]。
Romidepsin (0-48 hours；0-60 nM) 导致在 HCC 细胞中以时间和剂量依赖性方式诱导 G2/M 期细胞周期停滞^[2]。
Romidepsin (0-48 小时；0-60 nM) 在 HCC 细胞中促进细胞凋亡，增加 c-caspase-3、c-caspase-9 和 c-PARP 蛋白的表达^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	HCC cells
Concentration:	0 nM; 10 nM; 20 nM; 30 nM; 40 nM; 50 nM; 60 nM; 70 nM; 80 nM
Incubation Time:	0 hours; 12 hours; 24 hours; 48 hours; 72 hours
Result:	Inhibited HCC cells proliferation.

Cell Cycle Analysis^[2]

Cell Line:	HCC cells
Concentration:	0 nM; 15 nM; 30 nM; 60 nM
Incubation Time:	12 hours;24 hours; 48 hours
Result:	Caused a G2/M arrest.

Western Blot Analysis^[2]

Cell Line:	HCC cells
Concentration:	0 nM; 15 nM; 30 nM; 60 nM
Incubation Time:	12 hours;24 hours; 48 hours
Result:	Increaesd c-caspase-3, c-caspase-9, and c-PARP expression in HCC cells.

体内研究
(In Vivo)

Romidepsin (腹腔注射；0.5 和 1 mg/kg；每 3 天一次；21 天) 抑制肿瘤生长，显示 p-cdc25C、ki67、c-caspase-3 和 c-PARP 的高表达，以及降低肿瘤中 Ki-67 的表达^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Nude mice with Huh7 cells^[2]
Dosage:	0.5 and 1 mg/kg
Administration:	Intraperitoneal injection; 0.5 and 1 mg/kg; every 3 day; 21 days
Result:	Suppressed tumor growth in mouse xenograft models.

Clinical Trial

分子量

540.70

Formula

C₂₄H₃₆N₄O₆S₂

CAS 号

128517-07-7

性状

固体

颜色

White to off-white

中文名称

罗米地辛

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (184.95 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8495 mL	9.2473 mL	18.4945 mL
5 mM	0.3699 mL	1.8495 mL	3.6989 mL
10 mM	0.1849 mL	0.9247 mL	1.8495 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.85 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (3.85 mM); 悬浊液; 超声助溶

此方案可获得 2.08 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.85 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.98%

选择批次：

Data Sheet (672 KB) SDS (394 KB)

COA (304 KB) HNMR (211 KB) LCMS (139 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Furumai R, et al. FK228 (depsipeptide) as a natural prodrug that inhibits class I histone deacetylases. Cancer Res. 2002 Sep 1;62(17):4916-21. [Content Brief]

[2]. Sun WJ, et al. Romidepsin induces G2/M phase arrest via Erk/cdc25C/cdc2/cyclinB pathway and apoptosis induction through JNK/c-Jun/caspase3 pathway in hepatocellular carcinoma cells. Biochem Pharmacol. 2017 Mar 1;127:90-100. [Content Brief]

Romidepsin 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8495 mL	9.2473 mL	18.4945 mL	46.2364 mL
	5 mM	0.3699 mL	1.8495 mL	3.6989 mL	9.2473 mL
	10 mM	0.1849 mL	0.9247 mL	1.8495 mL	4.6236 mL
	15 mM	0.1233 mL	0.6165 mL	1.2330 mL	3.0824 mL
	20 mM	0.0925 mL	0.4624 mL	0.9247 mL	2.3118 mL
	25 mM	0.0740 mL	0.3699 mL	0.7398 mL	1.8495 mL
	30 mM	0.0616 mL	0.3082 mL	0.6165 mL	1.5412 mL
	40 mM	0.0462 mL	0.2312 mL	0.4624 mL	1.1559 mL
	50 mM	0.0370 mL	0.1849 mL	0.3699 mL	0.9247 mL
	60 mM	0.0308 mL	0.1541 mL	0.3082 mL	0.7706 mL
	80 mM	0.0231 mL	0.1156 mL	0.2312 mL	0.5780 mL
	100 mM	0.0185 mL	0.0925 mL	0.1849 mL	0.4624 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Romidepsin (Synonyms: 罗米地辛; FK 228; FR 901228; NSC 630176)

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MCE 顾客使用本产品发表的 65 篇科研文献

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完整储备液配制表

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Romidepsin (Synonyms: 罗米地辛; FK 228; FR 901228; NSC 630176)

Customer Review

Other Forms of Romidepsin:

Romidepsin 相关抗体

MCE 顾客使用本产品发表的 65 篇科研文献

Romidepsin 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 HDAC 亚型特异性产品:

生物活性

纯度 & 产品资料

参考文献

Romidepsin 相关抗体:

摩尔计算器

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Romidepsin 相关分类

完整储备液配制表

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