1. Apoptosis
    Autophagy
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  2. Apoptosis
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    mTOR
    Akt
  3. Cyclovirobuxine D

Cyclovirobuxine D 

目录号: HY-N0107 纯度: >95.0%
产品使用指南

Cyclovirobuxine D (CVB-D) 是中药黄杨 Buxus microphylla 的主要活性成分。Cyclovirobuxine D 诱导自噬并减弱 AktmTOR 的磷酸化。Cyclovirobuxine D 通过抑制细胞周期进程和诱导线粒体介导的细胞凋亡 apoptosis 抑制癌细胞的增殖。Cyclovirobuxine D 治疗心肌梗死引起的心力衰竭,具有治疗心力衰竭的潜力。

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Cyclovirobuxine D Chemical Structure

Cyclovirobuxine D Chemical Structure

CAS No. : 860-79-7

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Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥500 In-stock
5 mg ¥500 In-stock
10 mg ¥800 In-stock
20 mg ¥1400 In-stock
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Description

Cyclovirobuxine D (CVB-D) is the main active component of the traditional Chinese medicine Buxus microphylla. Cyclovirobuxine D induces autophagy and attenuates the phosphorylation of Akt and mTOR[1]. Cyclovirobuxine D inhibits cell proliferation of gastric cancer cells through suppression of cell cycle progression and inducement of mitochondria-mediated apoptosis[2]. Cyclovirobuxine D is beneficial for heart failure induced by myocardial infarction[3].

In Vitro

Cyclovirobuxine D (0-240 µM ;24-72 hours) shows a concentration- and time-dependent reduced cell viability after CVB-D treatment, only 10% MGC-803 cells and 20% MKN28 cells are alive at 72 h after treatment with 240 µM[2].
Cyclovirobuxine D (0-120 µM; 48 hours) arrests the cell cycle of gastric cancer cells at S phase in a concentration-dependent manner[2].
Cyclovirobuxine D (0-120 µM; 48 hours) leads to apoptosis in gastric cancer cells in a concentration-dependent manner, especially early stage apoptosis. Cyclovirobuxine D (0-120 µM; 48 hours) causes apoptosis via up-regulation of the apoptosis- related proteins, cleaved Caspase-3 and ratio of Bax/Bcl-2, in gastric cancer cells[2].

Cell Viability Assay[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, 120 and 240 µM
Incubation Time: 24, 48, 72 hours
Result: Reduced Cell Viability and Colony Formation Ability of Gastric Cancer Cells

Cell Cycle Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Arrested cell cycle progressions of MGC-803 and MKN28 cells.

Apoptosis Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Induced apoptosis of MGC-803 and MKN28 cells.

Western Blot Analysis[2]

Cell Line: MGC-803 and MKN28 cells
Concentration: 0, 30, 60, and 120 µM
Incubation Time: 48 hours
Result: Up-regulated cleaved Caspase-3 and Bax and decreased the expression of Bcl-2 expression.
Molecular Weight

402.66

Formula

C₂₆H₄₆N₂O

CAS No.

860-79-7

SMILES

C[C@H](NC)[C@@]1([H])[C@H](O)C[C@@]2(C)[C@]3([H])CC[C@@]4([H])C(C)(C)[C@@H](NC)CC[C@]4(C5)[C@]35CC[C@@]21C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 13 mg/mL (32.29 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4174 mL 24.8348 mL
5 mM 0.4967 mL 2.4835 mL 4.9670 mL
10 mM 0.2483 mL 1.2417 mL 2.4835 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。 -80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

    此方案可获得 ≥ 1.08 mg/mL (2.68 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.8 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,澄清透明的生理盐水溶液
  • 2.

    请依序添加每种溶剂: 10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

    此方案可获得 ≥ 1.08 mg/mL (2.68 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 10.8 mg/mL 的澄清 EtOH 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定溶至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% EtOH    90% corn oil

    Solubility: ≥ 1.08 mg/mL (2.68 mM); Clear solution

    此方案可获得 ≥ 1.08 mg/mL (2.68 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 10.8 mg/mL 的澄清 EtOH 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
References
  • 摩尔计算器

  • 稀释计算器

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Keywords:

Cyclovirobuxine DApoptosisAutophagymTORAktMammalian target of RapamycinPKBProtein kinase BInhibitorinhibitorinhibit

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