2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from E. coli K-235

Lipopolysaccharides, from E. coli K-235  (Synonyms: LPS, from Escherichia coli (K-235))

目录号: HY-D1056A5
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Lipopolysaccharides, from E. coli (Escherichia coli) K-235 是来源于大肠杆菌 (E. coli) 的脂多糖内毒素和 TLR-4 激活剂，是一种 S 型 LPS，可激活免疫系统的致病相关分子模式 (PAMP) 和诱导细胞分泌迁移体。Lipopolysaccharides, from E. coli K-235 具有典型的 3 部分结构：O 抗原 (O-antigen)、核心寡糖 (core oligosaccharide) 和脂质 A (Lipid A)。Lipopolysaccharides, from E. coli K-235 对 C57BL/10ScN 脾细胞有促有丝分裂作用，同时，用丁醇和脱氧胆酸法纯化过的 LPS 对 C57BL/10ScCR 和 C3H/HeJ 小鼠的脾细胞有刺激作用。
建议配制 ≥2 mg/mL 母液，并保证充分混合溶解。由于 LPS 具有吸附特性，分装保存时需使用低吸附离心管。

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Lipopolysaccharides, from E. coli K-235

Lipopolysaccharides, from E. coli K-235 Chemical Structure

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Lipopolysaccharides, from E. coli K-235 相关抗体

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生物活性

Lipopolysaccharides, from E. coli (Escherichia coli) K-235 are lipopolysaccharide endotoxins and TLR-4 activators derived from E. coli, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from E. coli K-235 exhibit a typical three-part structure: O-antigen (O-antigen), core oligosaccharide (core oligosaccharide), and lipid A (Lipid A). Lipopolysaccharides, from E. coli K-235 have a mitogenic effect on C57BL/10ScN spleen cells. Additionally, LPS purified using butanol and deoxycholic acid methods stimulates spleen cells in C57BL/10ScCR and C3H/HeJ mice[1][2].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

IC50 & Target

TLR-4[2]
体外研究
(In Vitro)

Note:
1. 为了保持 LPS 的完整性，建议将 LPS 溶液存放在硅烷化容器中。因为 LPS 可以粘附在塑料和某些类型的玻璃上，尤其是在浓度低于 0.1 mg/mL 时。如果 LPS 浓度超过 1 mg/mL，这种吸附作用相对较小，建议配制 ≥2 mg/mL 母液，并保证充分混合溶解。如果使用玻璃容器，请确保使用前充分混合至少 30 分钟，以重新溶解任何吸附在管壁的 LPS。
2. LPS 是一种能在溶液中形成不同尺寸胶束的分子，分子量不固定，储备液及工作液可直接配制成质量浓度 (mg/mL, μg/mL, etc.)。
3. LPS 溶于水或 PBS 后可能会观察到均匀的浑浊溶液，如需过滤除菌，请勿直接过滤储备液，建议稀释成工作液之后用 0.22 μm 的滤膜过滤除菌。

Lipopolysaccharides, from E. coli K-235 以 TLR4 和 CD14 依赖性的方式刺激巨噬细胞中促炎因子表达[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from E. coli K-235 相关抗体:
中文名称

脂多糖,来源于大肠杆菌K-235
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Lipopolysaccharides, from E. coli K-235 相关分类

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from E. coli K-235LPS, from Escherichia coli (K-235)脂多糖,来源于大肠杆菌K-235Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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