2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. Lipopolysaccharides, from Proteus mirabilis

Lipopolysaccharides, from Proteus mirabilis  (Synonyms: LPS, from bacterial (Proteus mirabilis))

目录号: HY-D1056B2
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Lipopolysaccharides, from Proteus mirabilis 是来源于奇异变形杆菌 (Proteus mirabilis) 的脂多糖内毒素和 TLR-4 激活剂，是 S 型 LPS，可激活免疫系统的致病相关分子模式 (PAMP) 和诱导细胞分泌迁移体。Lipopolysaccharides, from Proteus mirabilis 具有典型的 3 部分结构：O 抗原、核心寡糖和脂质 A。Proteus mirabilis 是导致尿路感染的主要病原体，并可能引起类风湿性关节炎。Lipopolysaccharides, from Proteus mirabilis 还具有潜在抗肿瘤作用，对实体肿瘤 (如脑膜肉瘤和 Walker 癌肉瘤) 具有体内抑制活性。
建议配制 ≥2 mg/mL 母液，并保证充分混合溶解。由于 LPS 具有吸附特性，分装保存时需使用低吸附离心管。

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Lipopolysaccharides, from Proteus mirabilis Chemical Structure

Lipopolysaccharides, from Proteus mirabilis Chemical Structure

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1 mg ￥3120
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Lipopolysaccharides, from Proteus mirabilis 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Lipopolysaccharides, from Proteus mirabilis are lipopolysaccharide endotoxins and TLR-4 activators derived from Proteus mirabilis, classified as S-type LPS, which can activate pathogen-associated molecular patterns (PAMP) of the immune system and induce cellular secretion of migrasomes. Lipopolysaccharides, from Proteus mirabilis exhibit a typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Proteus mirabilis is a major pathogen causing urinary tract infections and may also contribute to rheumatoid arthritis. Lipopolysaccharides, from Proteus mirabilis also exhibit potential anti-tumor effects, demonstrating in vivo inhibitory activity against solid tumors such as meningosarcoma and Walker carcinosarcoma[1][2].
It is recommended to prepare a stock solution of ≥2 mg/mL and ensure that it is fully mixed and dissolved. Due to the adsorption characteristics of LPS, low adsorption centrifuge tubes should be used for aliquoting and storage.

IC50 & Target

TLR-4[2]
体外研究
(In Vitro)

Note:
1. 为了保持 LPS 的完整性，建议将 LPS 溶液存放在硅烷化容器中。因为 LPS 可以粘附在塑料和某些类型的玻璃上，尤其是在浓度低于 0.1 mg/mL 时。如果 LPS 浓度超过 1 mg/mL，这种吸附作用相对较小，建议配制 ≥2 mg/mL 母液，并保证充分混合溶解。如果使用玻璃容器，请确保使用前充分混合至少 30 分钟，以重新溶解任何吸附在管壁的 LPS。
2. LPS 是一种能在溶液中形成不同尺寸胶束的分子，分子量不固定，储备液及工作液可直接配制成质量浓度 (mg/mL, μg/mL, etc.)。
3. LPS 溶于水或 PBS 后可能会观察到均匀的浑浊溶液，如需过滤除菌，请勿直接过滤储备液，建议稀释成工作液之后用 0.22 μm 的滤膜过滤除菌。

血清介导的免疫作用是通过激活补体级联信号，产生溶解复合物 C5b-9，发挥自身杀菌活性。Lipopolysaccharides, from Proteus mirabilis 则能够激活补体，并引起 C5b 沉积。Lipopolysaccharides, from Proteus mirabilis 与血清孵育 3 小时 (37 °C) 后，细菌活力受到抑制[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Lipopolysaccharides, from Proteus mirabilis 相关抗体:
体内研究
(In Vivo)

Lipopolysaccharides, from Proteus mirabilis (最低有效剂量 0.005 mg/kg；静脉注射或腹腔注射；单剂量) 在小鼠和大鼠荷瘤模型中，有效抑制实体型艾氏癌 (Ehrlich carcinoma) (ED=0.005 mg/kg)、Sarcoma 180、Sarcoma 37 等多种实体瘤，并使部分肿瘤完全消退。但 Lipopolysaccharides, from Proteus mirabilis 对腹水型肿瘤无效。Lipopolysaccharides, from Proteus mirabilis 能阻断肿瘤组织血流，使注入的物质 “Lock out” 或 “Lock in”。Lipopolysaccharides, from Proteus mirabilis 还可以与 6-mercaptopurine (HY-13677) 和 Cyclophosphamide (HY-17420) 等抗肿瘤药物联合使用，提高对 Meningeal sarcoma MS-147 和 Walker carcinosarcoma 256 等难治性肿瘤的抑制效果[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice bearing solid-type Ehrlich carcinoma, Sarcoma 180, Sarcoma 37, Meningeal sarcoma MS-147, Walker carcinosarcoma 256, Adenocarcinoma 755, Yoshida sarcoma, C3H spontaneous sarcoma, Lymphoid leukemia L 1210; Rats bearing Walker carcinosarcoma 256[2].
Dosage: LPS from wild type and heptoseless mutant N-434 of Proteus mirabilis RMS-203, with doses varying in different experiments.
Administration: Intravenous or intraperitoneal injection, subcutaneous injection (for some experiments); evaluating tumor regression in 2-4 weeks.
Result: LPS from both wild type and heptoseless mutant of Proteus mirabilis RMS-203 had the same level of activity when given intravenously in the Pigment test on Ehrlich solid tumor.
Inhibited the growth of solid-type Ehrlich carcinoma, Sarcoma 180, and Sarcoma 37, and could cause complete regression of these tumors.
Exhibited a vascular effect on tumor tissues, it could not be ineffective against ascites-type tumors.
同用名

LPS, from bacterial (Proteus mirabilis)

中文名称

脂多糖,来源于奇异变形杆菌
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Lipopolysaccharides, from Proteus mirabilis 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Lipopolysaccharides, from Proteus mirabilisLPS, from bacterial (Proteus mirabilis)脂多糖,来源于奇异变形杆菌Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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