1. Metabolic Enzyme/Protease
    Apoptosis
  2. Phosphatase
    Apoptosis
  3. NSC 95397

NSC 95397 

目录号: HY-108543
产品使用指南

NSC 95397 是一种有效选择性的 Cdc25 双特异性磷酸酶抑制剂 (Cdc25A、Cdc25B 以及 Cdc25C 的 Ki 值分别为 32、96、40 nM,人亚型 Cdc25A、人亚型 Cdc25C 以及 Cdc25B 的 IC50 值分别为 22.3、56.9、125 nM)。NSC 95397 抑制丝裂原活化蛋白激酶磷酸酶-1 (MKP-1),并通过 MKP-1 和 ERK1/2 途径抑制结肠癌细胞的增殖并诱导细胞凋亡。

MCE 的所有产品仅用作科学研究,我们不为任何个人用途提供产品和服务

NSC 95397 Chemical Structure

NSC 95397 Chemical Structure

CAS No. : 93718-83-3

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Stock
1 mg   Get quote  
5 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

Description

NSC 95397 is a potent, selective Cdc25 dual specificity phosphatase inhibitor (Ki=32 nM (Cdc25A), 96 nM (Cdc25B), 40 nM (Cdc25C); IC50=22.3 nM (human Cdc25A), 56.9 nM (human Cdc25C), 125 nM (Cdc25B))[1]. NSC 95397 inhibits mitogen-activated protein kinase phosphatase-1 (MKP-1) and suppresses proliferation and induces apoptosis in colon cancer cells through MKP-1 and ERK1/2 pathway[2].

IC50 & Target

Ki: 32 nM (Cdc25A), 96 nM (Cdc25B), 40 nM (Cdc25C)[1]
IC50: 22.3 nM (human Cdc25A) , 56.9 nM (human Cdc25C), 125 nM (Cdc25B)[1]

In Vitro

NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner[2].
NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells[2].
NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811[2].
NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels[2].
NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204[2].

Cell Viability Assay[2]

Cell Line: Human colon cancer cell lines, SW480, SW620, and DLD-1
Concentration: 0, 10, and 20 µM
Incubation Time: 24 hours
Result: The IC50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively.

Western Blot Analysis[2]

Cell Line: SW480, SW620, and DLD-1 cells
Concentration: 10 μM
Incubation Time: 24 hours
Result: p21 was upregulated while CDK4 and CDK6 were downregulated.
Rduced the phosphorylation of Rb on Ser795 and Ser807/811
Molecular Weight

310.39

Formula

C₁₄H₁₄O₄S₂

CAS No.

93718-83-3

SMILES

O=C1C(SCCO)=C(SCCO)C(C2=C1C=CC=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
NSC 95397
目录号:
HY-108543
需求量:

NSC 95397

Cat. No.: HY-108543