1. Cell Cycle/DNA Damage
    Epigenetics
  2. Sirtuin
  3. Thiomyristoyl

Thiomyristoyl 

目录号: HY-101278 纯度: 98.37%
产品使用指南

Thiomyristoyl是高效特异性的SIRT2抑制剂,IC50值为28 nM。

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Thiomyristoyl Chemical Structure

Thiomyristoyl Chemical Structure

CAS No. : 1429749-41-6

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥2368
In-stock
5 mg ¥1850
In-stock
10 mg ¥2950
In-stock
25 mg ¥5900
In-stock
50 mg ¥9800
In-stock
100 mg
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Top Publications Citing Use of Products

    Thiomyristoyl purchased from MCE. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. 

    HuCCT1 and RBE cells are treated with 0, 25, or 50 μm Tm (Thiomyristoyl) and subjected to western blotting

    Thiomyristoyl purchased from MCE. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. 

    After treatment with Tm (Thiomyristoyl), NAC, or a combination of both, the levels of ROS and proteins are detected.

    Thiomyristoyl purchased from MCE. Usage Cited in: Neoplasia. 2019 Mar 29;21(5):429-441. 

    cMYC, p-PDHA1, PHGDH, PSAT1 and PSPH protein levels in xenograft tissues from control and Tm (Thiomyristoyl) group are detected by staining and Western blotting.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Thiomyristoyl is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM.

    IC50 & Target

    SIRT2

    28 nM (IC50)

    SIRT1

    98 μM (IC50)

    体外研究
    (In Vitro)

    Thiomyristoyl (TM) is a potent SIRT2-specific inhibitor with broad anticancer activity but little effect on non-cancerous cells. SIRT2-inhibition promotes c-Myc ubiquitination and degradation, suggesting the therapeutic potential of TM to target certain c-Myc-driven cancers. TM could inhibit SIRT2 with an IC50 of 28 nM, but inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM. TM inhibits three human breast cancer cell lines, MCF-7, MDA-MB-468, and MDA-MB-231[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    TM inhibits tumor growth in mouse models of breast cancer. TM does not cause significant toxicity in mice and no significant weight loss is observed in TM-treated mice. S5H, the acetyl-a-tubulin level is moderately but statistically significantly increased in tumors from TM-treated mice compared with those from vehicle-treated mice, suggesting that TM indeed inhibits SIRT2 in vivo[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    581.85

    Formula

    C34H51N3O3S

    CAS 号
    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO : ≥ 32 mg/mL (55.00 mM)

    Ethanol : 15.29 mg/mL (26.28 mM; Need ultrasonic)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.7187 mL 8.5933 mL 17.1866 mL
    5 mM 0.3437 mL 1.7187 mL 3.4373 mL
    10 mM 0.1719 mL 0.8593 mL 1.7187 mL
    *

    请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

    In Vivo:

    请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
    分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 1.

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      此方案可获得 ≥ 2.5 mg/mL (4.30 mM,饱和度未知) 的澄清溶液。

      以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

      将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液
    • 2.

      请依序添加每种溶剂: 10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.30 mM); Clear solution

      此方案可获得 ≥ 2.5 mg/mL (4.30 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

      以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

    *以上所有助溶剂都可在 MCE 网站选购。
    参考文献
    Cell Assay
    [1]

    Cells are seeded into 96-well plates at 3,000–4,000 cells per well. After 24 hr, test compounds (Thiomyristoyl) are added to cells to final concentrations ranging from 1 to 50 μM. Cells are then incubated for 72 hr and cell viability is measured using the CellTiter-Blue viability assay. Relative cell viability in the presence of test compounds is normalized to the vehicle-treated controls after background subtraction. GraphPad Prism software is used to determine the IC50 values. Knockdown of SIRT1-7 in various cell lines is achieved by lentiviral infection[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献
    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2

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    产品名称:
    Thiomyristoyl
    目录号:
    HY-101278
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