2. Metabolic Enzyme/Protease Apoptosis Anti-infection
  3. Cytochrome P450 Apoptosis Parasite
  4. Columbamine chloride

Columbamine chloride  (Synonyms: Columbamin chloride; Dehydroisocorypalmine chloride)

目录号: HY-N0926A 纯度: 99.62%
COA 产品使用指南 技术支持

Columbamine (Columbamin; Dehydroisocorypalmine) chloride 是一种从植物中发现的有机杂四环生物碱。Columbamine chloride 是 Berberine (HY-N0716) 的代谢物。Columbamine chloride 抑制细胞色素 P450 (CYP) 亚型 CYP3A4 (IC50 = 30.6 µM)。Columbamine chloride 诱导癌细胞凋亡 (apoptosis)。Columbamine chloride 可用于抗氧化、抗炎、抗肿瘤、抗真菌、抗原虫、保肝、保神经、降血脂、降血糖等研究。

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Columbamine chloride

Columbamine chloride Chemical Structure

CAS No. : 1916-10-5

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1 mg ￥500
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5 mg ￥1400
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10 mg ￥2200
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Other Forms of Columbamine chloride:

Columbamine chloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 Cytochrome P450 亚型特异性产品:

查看所有亚型
CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A4 CYP17A1

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Columbamine (Columbamin; Dehydroisocorypalmine) chloride is an organic heterotetracyclic alkaloid extracted from plants. Columbamine chloride is a metabolite of Berberine (HY-N0716). Columbamine chloride inhibits the cytochrome P450 (CYP) isoform CYP3A4 (IC50 = 30.6 µM). Columbamine chloride induces apoptosis in cancer cells. Columbamine chloride can be used for antioxidant, anti-inflammatory, antitumor, antifungal, antiparasite, hepatoprotective, neuroprotective, hypolipidemic and hypoglycemic study[1][2][3][4][5][6].

IC50 & Target

CYP3A4

30.6 μM (IC50)

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
KB IC50
77.9 μM
Compound: Columbamine chloride
Cytotoxicity against human KB cells after 72 hrs
Cytotoxicity against human KB cells after 72 hrs
[PMID: 11141105]
体外研究
(In Vitro)

Columbamine (M2) (15 μM, 24 h) chloride 可降低 Hep G2 细胞内的甘油三酯 (TG) 含量[2]。
Columbamine (15 μM, 24 h) chloride 在 Hep G2 细胞中通过 AMPK 激活导致脂质合成减少和脂肪酸氧化增加[2]。
Columbamine (10-50 μM, 24-72 h) chloride 通过抑制 Wnt/β-catenin 信号通路抑制结肠癌细胞增殖[4]。
Columbamine (20-40 μM, 24 h) chloride 可抑制结肠癌细胞的侵袭和迁移[4]。
Columbamine (20-40 μM, 48 h) chloride 在结肠癌细胞中通过抑制 caspase 依赖机制触发细胞凋亡[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Columbamine chloride 相关抗体:

Cell Proliferation Assay[4]

Cell Line: LoVo, HCT-116, SW480 (Human colorectal cancer cell lines)
Concentration: 10, 20, 30, 40, 50 μM
Incubation Time: 24, 48, 72 h
Result: Inhibited the growth of the colon cells, which were proportional to treatment time and dosage.
Suppressed clone formation of HCT116 cells in a concentration and time-dependent manner.

Cell Migration Assay [4]

Cell Line: LoVo, HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 24 h
Result: Significantly inhibited the migration and invasion of HCT116 and Lovo cells compared with the control cells.
Greatly impaired the invasion and migration of HCT116 cells in dose-dependent manner.
Suppressed the expression of N-Cadherin and promoted the expression of E-cadherin, resulting in the inhibition of epithelial-mesenchymal transition (EMT) of colon cancer cells.
Attenuated expressions of MMP2, MMP7 and MMP9 proteins.

Apoptosis Analysis[4]

Cell Line: HCT-116
Concentration: 20, 30, 40 μM
Incubation Time: 48 h
Result: Significantly prevented cell growth and promoted cell death at concentration from 20 μM to 40 μM.
Increased the population of Annexin V-positive cells.
The cleaved form of caspase-3 and PARP were increased without affecting the expression of caspase-3 and PARP.
Promoted pro-apoptosis factor BAD expression and repressed anti-apoptosis factor Bcl-2 expression.

RT-PCR[2]

Cell Line: Hep G2 cells
Concentration: 15 μM
Incubation Time: 24 h
Result: Inhibited the expression of genes, such as FAS, HMGR, ACC and GPAT, which are associated with fat synthesis.
Increased the gene expression of mCAD, but had no effects on CPT-1.
体内研究
(In Vivo)

Columbamine (5-20 mg/kg；给药 23 天) 在 HCT116 异种移植小鼠模型中以剂量依赖性方式显著减小了肿瘤体积[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Six-weeks-old male BALB/c nude mice (subcutaneously injected with HCT116 and control cells on the opposite flanks of the same mouse)[4]
Dosage: 5, 10, 20 mg/kg
Administration: Administered to experimental mice for 23 days
Result: Decreased the tumor volumes in a dose-dependent manner.
分子量

373.83

Formula

C20H20ClNO4
CAS 号
性状

固体

颜色

Light yellow to yellow

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据
细胞实验： 

DMSO 中的溶解度 : 5 mg/mL (13.38 mM; 超声助溶 (<60°C); 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6750 mL 13.3751 mL 26.7501 mL
5 mM 0.5350 mL 2.6750 mL 5.3500 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.62%

COA (288 KB) HNMR (249 KB) LCMS (92 KB)

产品使用指南 (1538 KB)
参考文献

Columbamine chloride 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6750 mL 13.3751 mL 26.7501 mL 66.8753 mL
5 mM 0.5350 mL 2.6750 mL 5.3500 mL 13.3751 mL
10 mM 0.2675 mL 1.3375 mL 2.6750 mL 6.6875 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Columbamine1916-10-5ColumbaminDehydroisocorypalmineCytochrome P450ApoptosisParasiteCYPsdehydroisocorypalmineantioxidantanti-inflammatoryantitumorantimicrobialhepatoprotectiveneuroprotectivehypolipidemichypoglycemictriglycerideHep G2Inhibitorinhibitorinhibit

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目录号：
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