2. PI3K/Akt/mTOR Protein Tyrosine Kinase/RTK Cell Cycle/DNA Damage
  3. MELK PDGFR Haspin Kinase
  4. MELK-8a

MELK-8a  (Synonyms: NVS-MELK8a)

目录号: HY-100368
产品使用指南

摩尔计算器

MELK-8a (NVS-MELK8a) 是一种高效的选择性母体胚胎亮氨酸拉链激酶 (MELK) 抑制剂，IC50 为 2 nM。MELK-8a 还抑制 Flt3 (ITD)、Haspin、PDGFRα，IC50 分别为 0.18、0.19 和 0.42 μM。 MELK 在调节癌细胞的细胞有丝分裂中发挥着重要作用。

在相同的摩尔浓度下，化合物盐形式与游离形式有相同的生物活性，但盐形式 MELK-8a hydrochloride 通常具有更好的水溶性和稳定性。

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MELK-8a Chemical Structure

MELK-8a Chemical Structure

CAS No. : 1922153-17-0

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MELK-8a 的其他形式现货产品:

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MELK-8a 相关抗体

Top Publications Citing Use of Products

    MELK-8a purchased from MCE. Usage Cited in: J Biol Chem. 2020 Feb 21;295(8):2359-2374.  [Abstract]

    Asynchronous MDA-MB-468 cells are treated with DMSO or 0.5, 1, or 3 μM 8a for 1, 6, or 24h. Cells are harvested and immunoblotted with the indicated antibodies.

    MELK-8a purchased from MCE. Usage Cited in: J Biol Chem. 2020 Feb 21;295(8):2359-2374.  [Abstract]

    Delayed mitotic entry caused by 8a is associated with delayed activation of Aurora A, Aurora B, and CDK1. HeLaS3 cells are synchronized at G1/S with double thymidine block and released into DMSO or 3 μM 8a. Cells are collected starting at 6h post-release, with subsequent collections completed every 0.5 or 1h, over a 12h timecourse. Cells are lysed and immunoblotted with the indicated antibodies. The split in the image indicates that the samples from DMSO and 3 μM 8a conditions are blotted sepa.

    MELK-8a purchased from MCE. Usage Cited in: J Biol Chem. 2020 Feb 21;295(8):2359-2374.  [Abstract]

    MELK inhibition with 3 μM 8a causes delayed mitotic entry. HeLaS3 cells are synchronized at G1/S with double thymidine block and released into DMSO, 1 μM, or 3 μM 8a. Cells are collected every 2h over a 12h timecourse. Cells are lysed and immunoblotted with the indicated antibodies. The splits in the image indicate that the samples from DMSO, 1 μM, and 3 μM 8a conditions are immunoblotted separately due to space constraints. Asynchronous HeLaS3 lysate is loaded in the lanes labeled “A” in order

    MELK-8a purchased from MCE. Usage Cited in: J Biol Chem. 2020 Feb 21;295(8):2359-2374.  [Abstract]

    HeLaS3 cells treated with 8a complete mitosis and begin the subsequent cell cycle following delayed mitotic entry. HeLaS3 cells are synchronized at G1/S with double thymidine block and released into DMSO or 3 μM 8a. Cells are collected starting at 6h post-release, with subsequent collections completed every 2h over a 20h timecourse. Cells are lysed and immunoblotted with the indicated antibodies. The split in the image indicates that the samples from DMSO and 3 μM 8a conditions are blotted sepa

    MELK-8a purchased from MCE. Usage Cited in: Cell Rep. 2017 Dec 5;21(10):2829-2841.  [Abstract]

    A375 cells are treated with MELK-8a for 48 h and analyzed for indicated proteins by immunoblot analysis. ACTINB is used for as a loading control.

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    PDGFR PDGFRβ PDGFRα

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    MELK-8a (NVS-MELK8a) is a highly potent and selective maternal embryonic leucine zipper kinase (MELK) inhibitor with IC50 of 2 nM. MELK-8a also inhibits Flt3 (ITD), Haspin, PDGFRα with IC50s of 0.18, 0.19, and 0.42 μM, respectively. MELK plays an essential role in regulating cell mitosis in a subset of cancer cells[1].

    分子量

    432.56

    Formula

    C25H32N6O
    CAS 号
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    纯度 & 产品资料
    参考文献

    MELK-8a 相关分类

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    MELK-8a1922153-17-0NVS-MELK8aMELKPDGFRHaspin KinaseMaternal embryonic leucine zipper kinasePlatelet-derived growth factor receptormaternal embryonic leucine zipper kinaseMELK inhibitorcell mitosiscancer cellsInhibitorinhibitorinhibit

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