2. Protein Tyrosine Kinase/RTK
  3. FAK Pyk2
  4. PF-562271 hydrochloride

PF-562271 hydrochloride  (Synonyms: VS-6062(hydrochloride))

目录号: HY-20403 纯度: 98.10%
COA 产品使用指南 技术支持

PF-562271 (VS-6062) hydrochloride 是一种有效，可逆，ATP 竞争性的 FAK 和 Pyk2 激酶抑制剂，IC50 分别为 1.5 nM 和 13 nM。

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PF-562271 hydrochloride

PF-562271 hydrochloride Chemical Structure

CAS No. : 939791-41-0

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规格 价格 是否有货 数量
5 mg ￥1180
In-stock
10 mg   询价  
50 mg   询价  

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Customer Review

Other Forms of PF-562271 hydrochloride:

PF-562271 hydrochloride 相关抗体

Top Publications Citing Use of Products

    PF-562271 hydrochloride purchased from MCE. Usage Cited in: Cell Death Dis. 2023 Feb 24;14(2):157.  [Abstract]

    PF-562271 (250 nM; 6 h) largely inhibits Akt-S6 phosphorylation in OE-MXRA5 priPC-1 cells.

    PF-562271 hydrochloride purchased from MCE. Usage Cited in: Int J Cancer. 2015 Oct 1;137(7):1549-59.  [Abstract]

    786-O (a) and Caki-1 (b) are treated for 24 and 48h with increasing concentrations of PF-562,271 and adherent cells were counted. Cell lysates are evaluated through immunoblotting for total FAK following treatment for 24h.

    PF-562271 hydrochloride purchased from MCE. Usage Cited in: Practical Oncology Journal. 2015, 29(5): 444-449.

    Effects and mechanism of Biglycan and FAK signaling pathway on the invasion and metastasis of colon cancer cells.

    PF-562271 hydrochloride purchased from MCE. Usage Cited in: Cancer Res. 2013 May 1;73(9):2873-83.  [Abstract]

    FAK inhibition downregulates the AKT/mTOR pathway and CAS activity. A, protein levels measured by Western immunoblotting for AKT/mTOR pathway proteins in A673 and TC32 cells serum-starved overnight, treated with PF-562271 for 6 hours, and then stimulated with IGF-1 for 2 hours. Vinculin is used as the loading control. B, Western immunoblots showing downregulation of phospho-CAS but not phospho-ERK in A673 and TC32 cells after treatment with PF-562271.

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    PF-562271 (VS-6062) hydrochloride is a potent, ATP-competitive and reversible FAK and Pyk2 kinase inhibitor with IC50s of 1.5 nM and 13 nM, respectively[1].

    IC50 & Target

    IC50: 1.5 nM (FAK), 13 nM (Pyk2), 30 nM (CDK2), 47 nM (CDK3), 58 nM (CDK1), 97 nM (CDK7), 97 nM (Flt3)[1]
    体外研究
    (In Vitro)

    PF-562271 (VS-6062) hydrochloride is shown to be a 30- to 120-nM inhibitor of CDK2/E, CDK5/p35, CDK1/B, and CDK3/E in recombinant enzyme assays, in cell-based assays evaluating the role of CDKs, a 48-hour exposure of 3.3 μM PF-562271 is required to alter cell cycle progression. PF-562271 is potent in an inducible cell-based assay measuring phospho-FAK with a IC50 of 5 nM[1].
    PF-562271, a selective inhibitor of both FAK and proline-rich tyrosine kinase 2 (PYK2), a FAK-related family member, on cell growth and colony formation in Ewing sarcoma cell lines. Seven cell lines are treated for 5 days with PF-562271 across a range of concentrations using 2-fold serial dilutions. Treatment with PF-562271 impaires cell viability in all cell lines, with an average IC50 of 2.4 μM after 3 days of treatment. TC32 and A673 are the 2 most sensitive cell lines, with IC50 concentrations of 2.1 and 1.7 μM, respectively[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    PF-562271 hydrochloride 相关抗体:
    体内研究
    (In Vivo)

    PF-562271 inhibits FAK phosphorylation in vivo in a dose-dependent fashion (calculated EC50 of 93 ng/mL, total) after p.o. administration to tumor-bearing mice[1]. Rats that receive PF-562271 demonstrate a decrease in tumor growth after 2 weeks of treatment with signs of bone healing as evidenced by the deposition of new bone (cortical and cancellous) at sites previously damaged by the tumor[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    543.95

    Formula

    C21H21ClF3N7O3S
    CAS 号
    性状

    固体

    颜色

    Light yellow to yellow

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 100 mg/mL (183.84 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.8384 mL 9.1920 mL 18.3840 mL
    5 mM 0.3677 mL 1.8384 mL 3.6768 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料
    参考文献

    PF-562271 hydrochloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8384 mL 9.1920 mL 18.3840 mL 45.9601 mL
    5 mM 0.3677 mL 1.8384 mL 3.6768 mL 9.1920 mL
    10 mM 0.1838 mL 0.9192 mL 1.8384 mL 4.5960 mL
    15 mM 0.1226 mL 0.6128 mL 1.2256 mL 3.0640 mL
    20 mM 0.0919 mL 0.4596 mL 0.9192 mL 2.2980 mL
    25 mM 0.0735 mL 0.3677 mL 0.7354 mL 1.8384 mL
    30 mM 0.0613 mL 0.3064 mL 0.6128 mL 1.5320 mL
    40 mM 0.0460 mL 0.2298 mL 0.4596 mL 1.1490 mL
    50 mM 0.0368 mL 0.1838 mL 0.3677 mL 0.9192 mL
    60 mM 0.0306 mL 0.1532 mL 0.3064 mL 0.7660 mL
    80 mM 0.0230 mL 0.1149 mL 0.2298 mL 0.5745 mL
    100 mM 0.0184 mL 0.0919 mL 0.1838 mL 0.4596 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    PF-562271939791-41-0VS-6062(hydrochloride)PF562271PF 562271FAKPyk2PTK2 protein tyrosine kinase 2PTK2Focal adhesion kinaseProline-rich tyrosine kinase 2AntitumorEwingSarcomaBreastCancerInhibitorinhibitorinhibit

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    目录号：
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