1. PI3K/Akt/mTOR
  2. PI3K
  3. 740 Y-P

740 Y-P (Synonyms: 740YPDGFR; PDGFR 740Y-P)

目录号: HY-P0175 纯度: 96.49%
产品使用指南

740 Y-P (PDGFR 740Y-P) 是有效,可渗透细胞的 PI3K 活化物。

MCE 的所有产品仅用作科学研究,我们不为任何个人用途提供产品和服务

740 Y-P Chemical Structure

740 Y-P Chemical Structure

CAS No. : 1236188-16-1

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Size Price Stock Quantity
1 mg ¥1000 In-stock
5 mg ¥2800 In-stock
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50 mg   Get quote  

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Customer Review

Top Publications Citing Use of Products

    740 Y-P purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.

    PC12 cells are pretreated with 740-YP (30 μM), or SC79 (13.7 μM), or 3BDO (100 μM) for 1 h, and for the duration of GO (50 μg/mL) incubation for 24 h. LC3 turnover is detected by western blotting.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    Description

    740 Y-P (PDGFR 740Y-P) is a potent and cell permeable PI3K activator.

    IC50 & Target

    PI3K

     

    In Vitro

    740 Y-P is a synthetic biotinylated peptide consisting of the 16-amino acid sequence derived from the third helix of the Antennapedia protein in tandem with a 9 amino acid phosphopeptide mimetic of the high affinity p85 binding site on the PDGF receptor. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85. 740 Y-P fails to bind GST alone, or a GST fusion protein containing the N- terminal SH2 domain of PLCg and shows relatively weak binding of a GST fusion proteins containing the SH2 domain of Grb2. The 740 Y-P peptide is also able to specifically interact with p85 in cell lysates as shown by affinity precipitation. 740 Y-P stimulates mitogenesis at the lowest concentration tested (1 mg/mL). The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 mg/mL. 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides[1].

    In Vivo

    740 Y-P is not only internalised in living cells but can also interact with p85 in vivo.[1]

    Molecular Weight

    3270.70

    Formula

    C₁₄₁H₂₂₂N₄₃O₃₉PS₃

    CAS No.

    1236188-16-1

    Sequence

    Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Ser-Asp-Gly-Gly-{PO2-Tyr}-Met-Asp-Met-Ser

    Sequence Shortening

    R-Q-I-K-I-W-F-Q-N-R-R-M-K-W-K-K-S-D-G-G-{PO2-Y}-M-D-M-S

    SMILES

    [RQIKIWFQNRRMKWKKSDGG-{PO2-Tyr}-MDMS]

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 20 mg/mL (6.11 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.3057 mL 1.5287 mL 3.0574 mL
    5 mM 0.0611 mL 0.3057 mL 0.6115 mL
    10 mM --- --- ---
    * 请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液,并请注意储备液的保存方式和期限。
    References
    Cell Assay
    [1]

    Following serum starvation, C2 cells are cultured in the presence of the PDGFR 740Y-P peptide at the given concentrations (0, 1, 10, 100 μg/mL) for 48 h. The mitogenic response is assessed by measurement of the percentage of cells in S-phase[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 96.49%

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

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    Inquiry Information

    产品名称:
    740 Y-P
    目录号:
    HY-P0175
    需求量:

    740 Y-P

    Cat. No.: HY-P0175