2. Cell Cycle/DNA Damage Autophagy Apoptosis
  3. Antifolate Autophagy Apoptosis
  4. Pemetrexed disodium

Pemetrexed disodium  (Synonyms: 培美曲塞二钠盐; LY231514 disodium)

目录号: HY-10820A 纯度: 99.95%
COA 产品使用指南

摩尔计算器

Pemetrexed disodium (LY231514 disodium) 是一种叶酸拮抗剂 (antifolate)。Pemetrexed disodium 抑制胸苷酸合成酶 (TS)，二氢叶酸还原酶 (DHFR) 和甘氨酰胺核苷酸甲酰转移酶 (GARFT) 的 Ki 分别为 1.3 nM，7.2 nM 和 65 nM。

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Pemetrexed disodium Chemical Structure

Pemetrexed disodium Chemical Structure

CAS No. : 150399-23-8

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥605
In-stock
1 mg ￥171
In-stock
5 mg ￥343
In-stock
10 mg ￥550
In-stock
50 mg ￥750
In-stock
100 mg ￥1250
In-stock
200 mg ￥2100
In-stock
500 mg   询价  
1 g   询价  

* Please select Quantity before adding items.

Other Forms of Pemetrexed disodium:

Pemetrexed disodium 相关抗体

Top Publications Citing Use of Products

    Pemetrexed disodium purchased from MCE. Usage Cited in: Theranostics. 2020 May 15;10(13):6048-6060.  [Abstract]

    Immunofluorescence assay showing the expression and intracellular location of UCHL1 in NSCLC cells. Increased UCHL1 levels are observed in both the cytoplasm and the nucleus of the PEM-R cells.

    Pemetrexed disodium purchased from MCE. Usage Cited in: Theranostics. 2020 May 15;10(13):6048-6060.  [Abstract]

    Western blot analysis of UCHL1 levels in PEM-R cells and their parental cells. The mRNA and protein levels of UCHL1 in the PEM-R cells are significantly increased, relative to in the parental cells.

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Pemetrexed disodium (LY231514 disodium) is an antifolate, the Kis of the pentaglutamate of Pemetrexed disodium are 1.3, 7.2, and 65 nM for inhibits thymidylate synthase (TS), dihydrofolate reductase (DHFR), and glycinamide ribonucleotide formyltransferase (GARFT), respectively[1].

    IC50 & Target

    Ki: 1.3 nM (TS), 7.2 nM (DHFR), 65 nM (GARFT)[1]
    体外研究
    (In Vitro)

    Pemetrexed (LY231514) disodium is a novel classical antifolate, the antitumor activity of which may result from simultaneous and multipie inhibition of several key folate-requiring enzymes via its polyglutamated metabolites. Pemetrexed (LY231514) is one of the best substrates that is known for the enzyme FPGS (Km=1.6 μM and Vmax/Km=621). It is likely that polyglutamation and the polyglutamated metabolites of LY231514 play profound roles in determining both the selectivity and the antitumor activity of this novel agent. Whereas LY23l5l4 only moderately inhibits TS (Ki=340 nM, recombinant mouse), the pentaglutamate of LY23l5l4 is 100-fold more potent (Ki=3.4 nM), making LY231514 one of the most potent folate-based TS inhibitors[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Pemetrexed disodium 相关抗体:
    体内研究
    (In Vivo)

    The group of mice treated with PC61 plus Pemetrexed demonstrates statistically longer survival than other groups. In a survival analysis, significantly better survival is observed in the group of mice treated with PC61 plus Pemetrexed compare with those treated with PC61 alone, rat IgG plus Pemetrexed, or no treatment[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    471.37

    Formula

    C20H19N5Na2O6
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    培美曲塞二钠盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : 100 mg/mL (212.15 mM; 超声助溶 (<60°C))

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.1215 mL 10.6074 mL 21.2148 mL
    5 mM 0.4243 mL 2.1215 mL 4.2430 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案，请直接配置工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 50 mg/mL (106.07 mM); 澄清溶液; 超声助溶

    • 方案 二

      请依序添加每种溶剂： Saline

      Solubility: 100 mg/mL (212.15 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.95%

    COA (191 KB) HNMR (271 KB) LCMS (266 KB)

    产品使用指南 (1538 KB)
    参考文献
    Cell Assay
    [1]

    Dose-response curves are generated to determine the concentration required for 50% inhibition of growth (IC50). Pemetrexed is dissolved initially in DMSO at a concentration of 4 mg/mL and further diluted with cell culture medium to the desired concentration. CCRF-CEM leukemia cells in complete medium are added to 24-well Cluster plates at a final concentration of 4.8×104 cells/well in a total volume of 2 mL. Test compounds at various concentrations are added to duplicate wells so that the final volume of DMSO is 0.5%. The plates are incubated for 72 h at 37°C in an atmosphere of 5% CO2 in air. At the end of the incubation, cell numbers are determined on a ZBI Coulter counter. Control wells usually contain 4×105 to 6×105 cells at the end of the incubation. For several studies, IC50s are determined for each compound in the presence of either 300 μM AICA, 5 μM thymidine, 100 μM hypoxanthine, or combination of 5 μM hymidine plus 100 μM hypoxanthine[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    Female CBA mice and female NOD/SCID mice (NOD.CB17-Prkdcscid) at 6-8 wk of age are used. Premetrexed (100 mg/kg) is given i.p. from days 4-8 (5 consecutive d) to tumor-bearing mice to explore the synergistic effect when combined with anti-CD25 Ab or IgG control. The dose and schedule used for Pemetrexed in the current study is determined based on previous studies in mice.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Pemetrexed disodium 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.1215 mL 10.6074 mL 21.2148 mL 53.0369 mL
    5 mM 0.4243 mL 2.1215 mL 4.2430 mL 10.6074 mL
    10 mM 0.2121 mL 1.0607 mL 2.1215 mL 5.3037 mL
    15 mM 0.1414 mL 0.7072 mL 1.4143 mL 3.5358 mL
    20 mM 0.1061 mL 0.5304 mL 1.0607 mL 2.6518 mL
    25 mM 0.0849 mL 0.4243 mL 0.8486 mL 2.1215 mL
    30 mM 0.0707 mL 0.3536 mL 0.7072 mL 1.7679 mL
    40 mM 0.0530 mL 0.2652 mL 0.5304 mL 1.3259 mL
    50 mM 0.0424 mL 0.2121 mL 0.4243 mL 1.0607 mL
    60 mM 0.0354 mL 0.1768 mL 0.3536 mL 0.8839 mL
    80 mM 0.0265 mL 0.1326 mL 0.2652 mL 0.6630 mL
    100 mM 0.0212 mL 0.1061 mL 0.2121 mL 0.5304 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pemetrexed disodium150399-23-8LY231514 disodium培美曲塞二钠盐AntifolateAutophagyApoptosisInhibitorinhibitorinhibit

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