1. PI3K/Akt/mTOR
    Apoptosis
  2. mTOR
    Apoptosis
  3. Salidroside

Salidroside (Synonyms: Rhodioloside)

目录号: HY-N0109 纯度: 98.63%
产品使用指南

Salidroside 是一种脯氨酰内肽酶 (prolyl endopeptidase) 抑制剂。Salidroside 可通过激活 mTOR 信号缓解肿瘤恶病质小鼠模型中的恶病质症状。Salidroside 还能通过增强 PINK1/Parkin 介导的线粒体自噬来保护多巴胺能神经元。

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Salidroside Chemical Structure

Salidroside Chemical Structure

CAS No. : 10338-51-9

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Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1  mL in Water ¥605 In-stock
5 mg ¥550 In-stock
10 mg ¥880 In-stock
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100 mg   Get quote  

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Customer Review

Top Publications Citing Use of Products

    Salidroside purchased from MCE. Usage Cited in: Int J Oncol. 2019 Jun;54(6):1969-1980.

    Apoptosis‑related protein expression in MG63 cells in response to various concentrations of Salidroside is detected via western blot analysis.

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    • 参考文献

    Description

    Salidroside is a prolyl endopeptidase Inhibitor. Salidroside alleviates cachexia symptoms in mouse models of cancer cachexia via activating mTOR signalling. Salidroside protects dopaminergic neurons by enhancing PINK1/Parkin-mediated mitophagy.

    IC50 & Target[2]

    mTOR

     

    In Vitro

    Salidroside (100 μM) inhibits prolyl endopeptidase (PEP) activity (10.6±1.9%). Prolyl endopeptidase is an enzyme that plays a role in the metabolism of proline-containing neuropeptidase which is recognized to be involved in learning and memory[1]. Salidroside, one of the major phenylpropanoid glycosides found in R. rosea L, is consumed almost daily as a nutritional supplement in many countries and has been identified possessing potential anti-fatigue and anoxia,anti-aging, and anti-Alzheimer's disease activities. Salidroside can improve muscle nutrition via increasing mTOR, p-mTOR, and MyHC expression[2]. SH-SY5Y cells are exposed to 0-600 μM MPP+ for 12-48 h and the results show that MPP+ results in a significant decrease of cell viability in a concentration and time-dependent manner. Cells are pretreated with 25-100 μM Salidroside (Sal) for 24 h and then exposed to 500 μM MPP+ for an additional 24 h. Salidroside concentration-dependently prevents MPP+-induced decrease of cell viability. Annexin V/PI staining is a common method for the detection of apoptotic cell. Salidroside significantly decreases the number of Annexin V/PI-stained cells treated by MPP+ which is in a concentration-dependent manner. Apoptotic cell could also be morphologically evaluated by Hoechst staining. In Hoechst staining, apoptotic cells are characterized by reduced nuclear size, chromatin condensation, intense fluorescence, and nuclear fragmentation. Salidroside notably inhibits MPP+-induced increase of chromatin condensation, intense fluorescence, and nuclear fragmentation in SH-SY5Y cells[3].

    In Vivo

    Salidroside is a natural antioxidant extracted from medicinal food plant Rhodiola rosea. Salidroside (100 mg/kg/day) shows strong glucose lowering effect on db/db mice which is similar to effect of Metformin (200 mg/kg/day). For this reason, the dose of 100 mg/kg/day salidroside is used[4].

    Molecular Weight

    300.30

    Formula

    C₁₄H₂₀O₇

    CAS No.

    10338-51-9

    SMILES

    O[C@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)[C@@H]1OCCC2=CC=C(O)C=C2

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (333.00 mM)

    * "≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3300 mL 16.6500 mL 33.3000 mL
    5 mM 0.6660 mL 3.3300 mL 6.6600 mL
    10 mM 0.3330 mL 1.6650 mL 3.3300 mL
    *

    请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。 -80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

    References
    Cell Assay
    [3]

    SH-SY5Y cells are seeded in 96-well plates at 1×104 cells per well. After the treatment with Salidroside (25-100 μM) and MPP+, cell viability is measured by MTT assay. Briefly, cells are incubated with 500 μg/mL MTT at 37°C for 4 h. After that, the medium is removed and 150 μL DMSO is added and shaking is conducted for 10 min. Absorbance is measured at 570 nm in a microplate reader and the results are expressed as folds of control[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4]

    Mice[4]
    The 4-week-old male C57BL/6 mice are fed a high-fat diet (HFD) (n=16) or normal chow diet (n=8). After 10 weeks of the HFD, salidroside intervention (100 mg/kg/day) is initiated by gavage once a day for 5 weeks. The control groups are given vehicle (saline). The 4-week-old male C57Bl/KsJ (BKS) mice (wild type, n=8) and BKS.Cg-Dock7m +/+ Leprdb/J (db/db) mice (n=16) are used. Salidroside (100 mg/kg/day) is administrated orally by gavage once a day for 5 weeks. The control groups are given vehicle (saline). Fasting blood glucose and body weight of mice are monitored every 5 days. Glucose measurements are performed on blood drawn from the tail vein using a Glucometer.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.63%

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    Keywords:

    SalidrosideRhodiolosidemTORApoptosisMammalian target of RapamycinInhibitorinhibitorinhibit

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    产品名称:
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    目录号:
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