1. Anti-infection Stem Cell/Wnt Autophagy Apoptosis
  2. Bacterial Wnt β-catenin Mitophagy Autophagy Apoptosis Antibiotic Parasite
  3. Salinomycin

Salinomycin  (Synonyms: 盐霉素; Procoxacin)

目录号: HY-15597 纯度: 98.39%
COA 产品使用指南

Salinomycin (Procoxacin),一种钾离子载体抗生素,选择性抑制革兰氏阳性菌的生长 (gram-positive bacteria)。Salinomycin 是 Wnt/β-catenin 信号传导的有效抑制剂,阻断 Wnt 诱导的 LRP6 磷酸化。Salinomycin (Procoxacin) 选择性抑制人肿瘤干细胞。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

Salinomycin Chemical Structure

Salinomycin Chemical Structure

CAS No. : 53003-10-4

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥991
In-stock
1 mg ¥318
In-stock
5 mg ¥700
In-stock
10 mg ¥1200
In-stock
50 mg ¥3900
In-stock
100 mg   询价  
200 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Salinomycin:

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products

    Salinomycin purchased from MCE. Usage Cited in: Cell Biosci. 2021 Aug 4;11(1):156.  [Abstract]

    Salinomycin (0.0078125 μg/ml; for 24 h) reverses the MDLS-induced up-regulation of Oct4. The expression of Oct4 is determined using Western blot analysis.

    Salinomycin purchased from MCE. Usage Cited in: Cell Commun Signal. 2018 Nov 23;16(1):89.  [Abstract]

    The expression of collagen I, α-smooth muscle actin (α-SMA), and Sca-1 in lung tissues is measured by western blotting in the treatment of Saline, Salinomycin, Bleomycin+Vehicle, and Bleomycin+ Salinomycin.

    Salinomycin purchased from MCE. Usage Cited in: Oncol Rep. 2018 Aug;40(2):877-886.  [Abstract]

    Salinomycin (SAL) has a pro-apoptotic effect on NB4 and HL-60 cells. The apoptosis-related protein levels of Bax and Bcl-2 are assessed by western blotting with the β-actin protein as an internal control.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Salinomycin (Procoxacin), a polyether potassium ionophore antibiotic, selectively inhibits the growth of gram-positive bacteria. Salinomycin is a potent inhibitor of Wnt/β-catenin signaling, blocks Wnt-induced LRP6 phosphorylation. Salinomycin (Procoxacin) shows selective activity against human cancer stem cells[1][2][3].

    IC50 & Target

    Coccidia

     

    体外研究
    (In Vitro)

    Salinomycin 是 Wnt 信号级联的有效抑制剂。Salinomycin 孵育恶性淋巴细胞可在 48 小时内诱导细胞凋亡,平均 IC50 为 230 nM。Salinomycin 也是一种抗生素钾离子载体,最近有报道作为选择性乳腺癌干细胞抑制剂[1]
    Salinomycin 是一种新型有效的抗癌药物,抑制 SW620 细胞和 Cisp 抗性 SW620 细胞,IC50 分别为 1.54±0.23 μM 和 0.32±0.05 μM。发现 Salinomycin 具有杀死癌症干细胞 (CSC) 和耐药癌细胞的能力。连续用沙利霉素处理 48 h 后,显微镜下观察凋亡细胞,随机计数一个视野至少 100 个细胞。Cisp 抗性 SW620 细胞中 Hoechst33342 染色的凋亡细胞数 (20.20±3.72) 明显高于 SW620 细胞 (9.40±2.07)/100 个细胞 (p<0.05)。用 Salinomycin 处理 48 小时后,使用流式细胞术分析检测 SW620 细胞和 Cisp 抗性 SW620 细胞的细胞凋亡。抗 Cisp 的 SW620 细胞凋亡率 (37.82±3.63%) 明显高于 SW620 细胞 (16.78±2.56%) (p<0.05)[2]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    在施用 4 mg/kg Salinomycin (Sal)、8 mg/kg Salinomycin 和 10 μL/g 盐水 6 周后,处死小鼠。与对照组相比,Salinomycin 处理组中肝肿瘤的大小减小。肿瘤的平均直径从 12.17 mm 减小到 3.67 mm (p<0.05),肿瘤的平均体积 (V=长×宽2×0.5) 从 819 mm3 减小至 25.25 mm3 (p<0.05)。接下来,收获肿瘤,然后进行 HE 染色、免疫组织化学和 TUNEL 测定,以评估 Salinomycin 的抗肿瘤活性。HE染色显示肝癌组织结构:大小不一的细胞核,肝索结构被破坏。免疫组织化学显示 Salinomycin 处理后 PCNA 表达较低。HE染色和TUNEL测定表明 Salinomycin 处理组的细胞凋亡率高于对照组。此外,免疫组织化学显示 Salinomycin 处理后 Bax/Bcl-2 比率增加。与对照组相比,Salinomycin 处理组 β-catenin 蛋白表达降低[4]
    Salinomycin 是一种单羧酸聚醚类抗生素,由白色链霉菌发酵产生。它具有特殊的环状结构,能与病原微生物和球虫的胞外阳离子,尤其是 K+、Na+ 、Rb+ 形成络合物,改变细胞内外离子浓度[5]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    751.00

    Formula

    C42H70O11

    CAS 号
    性状

    固体

    颜色

    White to light yellow

    中文名称

    盐霉素;沙利霉素

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : ≥ 36.7 mg/mL (48.87 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 1.3316 mL 6.6578 mL 13.3156 mL
    5 mM 0.2663 mL 1.3316 mL 2.6631 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
    ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.33 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

      生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂: 10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (3.33 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL(饱和度未知)的澄清溶液,此方案实验周期在半个月以上的动物实验酌情使用。

      1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    请输入您的动物体内配方组成:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠,建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括:DMSO ,均可在 MCE 网站选购。 Tween 80,均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO(母液浓度为 mg/mL)。
    您所需的储备液浓度超过该产品的实测溶解度,以下方案仅供参考,如有需要,请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液,加入 μL  μL ,混合均匀至澄清,再加 μL Tween 80,混合均匀至澄清,再加 μL 生理盐水
    连续给药周期超过半月以上,请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态,从左到右依次添加助溶剂。您可采用超声加热 (超声清洗仪,建议频次 20-40 kHz),涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 98.39%

    参考文献
    Cell Assay
    [2]

    For cisplatin or Salinomycin IC50 analysis in SW620 cells or Cisp-resistant SW620 cells, cells (1×104/well) are cultured in 96-well plates and treated with different chemotherapeutics (cisplatin, Salinomycin) in different concentrations for 48 h. Then 20 μL of cell counting kit-8 (CCK-8) is added into each of the 96-wells. After 4 h incubation at 37°C, the optical density (OD) values are detected at 450 nm using the scan reader. Cell growth inhibiting rates are described as cell inhibiting curves and the IC50 parameters (inhibiting concentration of 50% cells) are evaluated by Xlfit 5.2 software. For cell proliferation analysis, SW620 cells or Cisp-resistant SW620 cells (5×103/well) are also seeded in 96-well plates in serum-containing medium and treated with cisplatin (5 μM, according to the calculated IC50 values of cisplatin in SW620 cells) for 0, 12, 24, 48, 72 and 96 h. Then 20 μL cell counting kit-8 is added into each of the 96-wells. After 4-h incubation at 37°C, the coloring reactions are also quantified at 450 nm[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3][4]

    Mice[3]
    Nude mice (nu/nu; 4-6 weeks of age) are used. HepG2 cells are suspended in 100 mL 1:1 serum-free DMEM and Matrigel. Mice are anesthetized with ketamine/xylazine and after surgically opening the abdomen, HepG2 cells are inoculated into the liver parenchyma and mice are monitored every 3 days for 35 days. Finally, 18 nude mice are divided into three groups that are intraperitoneally injected daily for 6 weeks: two Salinomycin-treated groups (4 mg/kg Salinomycin group, 8 mg/kg Salinomycin group) and the control group (saline water group).
    Rats[4]
    A total of 10 male rats are used in the experiment. After a routine anesthesia, the abdomen is opened. After a resuspension of high glucose medium not containing serum DMEM, and matrigel, the bladder transitional cancer cell line T24 is inoculated in the parenchyma of bladder in rats, and then the abdomen is sutured. After operation, the rats are randomized into the experiment group and the control group with five in each group. After operation, the rats in the experiment group are immediately given intraperitoneal injection of Salinomycin with a dosage of 8 mg/kg, while the rats in the control group are given intraperitoneal injection of normal saline. A close observation is paid during the drug administration period. After 15 d, the rats are sacrificed by cervical dislocation, and the complete tumor tissues are stripped to observe the tumor growth and metastasis.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3316 mL 6.6578 mL 13.3156 mL 33.2889 mL
    5 mM 0.2663 mL 1.3316 mL 2.6631 mL 6.6578 mL
    10 mM 0.1332 mL 0.6658 mL 1.3316 mL 3.3289 mL
    15 mM 0.0888 mL 0.4439 mL 0.8877 mL 2.2193 mL
    20 mM 0.0666 mL 0.3329 mL 0.6658 mL 1.6644 mL
    25 mM 0.0533 mL 0.2663 mL 0.5326 mL 1.3316 mL
    30 mM 0.0444 mL 0.2219 mL 0.4439 mL 1.1096 mL
    40 mM 0.0333 mL 0.1664 mL 0.3329 mL 0.8322 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    您最近查看的产品:

    Your information is safe with us. * Required Fields.

       产品名称:

     

    * 需求量:

    * 客户姓名:

     

    * Email:

    * 电话:

     

    * 公司或机构名称:

       留言给我们:

    Bulk Inquiry

    Inquiry Information

    产品名称:
    Salinomycin
    目录号:
    HY-15597
    需求量: