2. 诱导疾病模型产品 GPCR/G Protein Apoptosis
  3. 心血管系统疾病模型 Angiotensin Receptor Apoptosis
  4. 高血压模型
  5. Angiotensin II human acetate

Angiotensin II human acetate  (Synonyms: Angiotensin II acetate; Ang II acetate; DRVYIHPF acetate)

目录号: HY-13948A 纯度: 99.62%
COA 产品使用指南 技术支持

Angiotensin II human (Angiotensin II) acetate 是一种血管收缩剂，是肾素/血管紧张素系统的主要生物活性肽。Angiotensin II human acetate 在调节人类血压中起着核心作用，主要通过血管紧张素 II 与 G 蛋白偶联受体 (GPCRs)、血管紧张素II 1型受体 (AT1R) 和血管紧张素II 2型受体 (AT2R) 之间的相互作用来介导。Angiotensin II human acetate 刺激交感神经刺激，增加醛固酮生物合成和肾脏活动。Angiotensin II human acetate 诱导血管平滑肌细胞生长，增加成纤维细胞中 I 型和 III 型胶原的合成，导致血管壁和心肌增厚，并导致纤维化。Angiotensin II human acetate 也诱导细胞凋亡。Angiotensin II human acetate 通过 LOX-1 依赖的氧化还原敏感途径诱导内皮细胞毛细血管形成。

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Custom Peptide Synthesis

Angiotensin II human acetate

Angiotensin II human acetate Chemical Structure

CAS No. : 68521-88-0

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规格 价格 是否有货 数量
1 mg ￥188
In-stock
5 mg ￥470
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10 mg ￥750
In-stock
25 mg ￥1375
In-stock
50 mg ￥1900
In-stock
100 mg ￥2800
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Other Forms of Angiotensin II human acetate:

Angiotensin II human acetate 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 173 篇科研文献

WB
RT-PCR

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Mol Cell Endocrinol. 2023 Jul 1:571:111938.  [Abstract]

    Angiotensin II human (Ang II; 1 μM; 48 h) increases the expression of MFN1, t-CaMKⅡ and p-CaMKⅡ and decreases the expression of SERCA2 in NMCMs.

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Biomedicines. 2022 Dec 4;10(12):3131.

    Angiotensin II human (Ang II; 100 nM; 24 h) pretreatment significantly increases both the protein level of CtBP1 during 3T3-L1 adipogenesis.

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Biomedicines. 2022 Dec 4;10(12):3131.

    Angiotensin II human (Ang II; 100 nM; 24 h) pretreatment significantly increases both the mRNA level of CtBP1 during 3T3-L1 adipogenesis.

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Sci Transl Med. 2021 Jul 21;13(603):eaaz4959.  [Abstract]

    SMC was stimulated with vehicle and AngII of 10-8 M, 10-7 M, 10-6 M,10-5 M and 10-4 M, respectively, for 24 hrs. Cells were harvested and EPO protein abundance measured by Western blot.

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Sci Transl Med. 2021 Jul 21;13(603):eaaz4959.  [Abstract]

    Cultured human renal 786-O cells were stimulated with vehicle, AngII (50μM), AngII + Telmisartan (1μM), AngII + PD123319 (50μM) for 12 hrs. Cells were harvested and EPO protein abundance was detected by Western blot.

    Angiotensin II human acetate purchased from MCE. Usage Cited in: Int J Biochem Cell Biol. 2020 Apr;121:105703.  [Abstract]

    HPMCs are treated with 5.6 mM glucose (NG) or with 236 mM glucose (HG), or 100 nM AngII (AngII), or 236 mM glucose plus 10 mM valsartan (HGþvalsartan), or 100 nM AngII plus 10 mM valsartan (AngIIþvalsartan) for 24 h. The protein levels of p-mTOR, mTOR, p-4EBP1, 4EBP1, p-S6K1, and S6K1 are examined by Western blot analysis.

    查看 Angiotensin Receptor 亚型特异性产品:

    查看所有亚型
    AT1 Receptor AT2 Receptor

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Angiotensin II human (Angiotensin II) acetate is a vasoconstrictor and a major bioactive peptide of the renin/angiotensin system. Angiotensin II human acetate plays a central role in regulating human blood pressure, which is mainly mediated by interactions between Angiotensin II and the G-protein-coupled receptors (GPCRs) Angiotensin II type 1 receptor (AT1R) and Angiotensin II type 2 receptor (AT2R). Angiotensin II human acetate stimulates sympathetic nervous stimulation, increases aldosterone biosynthesis and renal actions. Angiotensin II human acetate induces growth of vascular smooth muscle cells, increases collagen type I and III synthesis in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. Angiotensin II human acetate also induces apoptosis. Angiotensin II human acetate induces capillary formation from endothelial cells via the LOX-1 dependent redox-sensitive pathway[1][2][3][4].

    IC50 & Target

    AT2 Receptor

     

    AT1 Receptor

     

    体外研究
    (In Vitro)

    Most of the known actions of Angiotensin II (Ang II) human acetate are mediated by AT1 receptors, the AT2 receptor contributes to the regulation of blood pressure and renal function[1].
    Angiotensin II human acetate raises blood pressure (BP) by a number of actions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal actions. Other Angiotensin II human acetate actions include induction of growth, cell migration, and mitosis of vascular smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT1)[2].
    Angiotensin II (1 nM) induces the expression of LOX-1 and VEGF and enhances capillary formation from human coronary endothelial cells in Matrigel assay. Angiotensin II -mediated expression of LOX-1 and VEGF, capillary formation, intracellular reactive oxygen species generation, and phosphorylation of p38 as well as p44/42 mitogen-activated protein kinases, were suppressed by anti-LOX-1 antibody, nicotinamide-adenine dinucleotide phosphate oxidase inhibitor apocynin and the Ang II type 1 receptor blocker Losartan, but not by the Ang II type 2 receptor blocker PD123319[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Angiotensin II human acetate 相关抗体:
    体内研究
    (In Vivo)

    注意：
    请勿仅参考一篇文章来确定实验条件。建议在正式实验前，通过预实验确定最佳实验条件 (动物品系、年龄、剂量、频率及周期、检测时间及指标等)。

    Angiotensin II human (acetate) 可用于诱导高血压、心脏肥大和腹主动脉瘤模型[7][8][9][10][11]。

    Angiotensin II (acetate) 通过受体介导的内吞作用被各种细胞类型内化，在心脏、肾脏和肾上腺等组织大量富集。骨骼肌组织中浓度为血浆浓度的 8%-41%，心脏为 64%-150%，肾脏为 340%-550%，肾上腺则高达 680%-2100%。血浆中的 Angiotensin II (acetate) 达到稳态浓度仅需约 5 分钟，而组织中达到稳态浓度需要 30-60 分钟。循环系统中 Angiotensin II (acetate) 的半衰期极短 (约 0.5 分钟)，而在心脏、肾脏和肾上腺组织中，内吞后形成的 Angiotensin II (acetate) 的体内半衰期显著延长至约 15 分钟[10]。
    1. 诱发高血压[7]
    致病原理
    Angiotensin II human 通过激活 AT1 受体，引起血管收缩、钠水潴留、交感神经激活及氧化应激，导致血压升高。
    具体造模方法：
    小鼠：C57/BL6J • 雄性&雌性 • 12-16 周龄 • 21-27 g
    给药方式：800 ng/kg/min, 0.003 mL/min • 7 天 • sc，皮下植入渗透泵
    Note
    性别的影响：慢性 ANG II 诱发的高血压，在清醒小鼠中存在性别差异。雌性小鼠可能免受 ANG II 引起的血压升高的影响。
    造模成功指标
    关键因素：血压↑ 第7天，男性血压高于女性。
    相关产品：/
    拮抗产品：/

    2. 诱发心脏肥大[8][9]
    致病原理
    Angiotensin II humanAng II 通过激活 AT1 受体，引发炎症、氧化应激和细胞外基质重塑，导致心肌细胞增生和肥厚。
    具体造模方法：
    小鼠：C57/BL6J • 雄性 • 8 周龄 &bull
    给药方式：2 μg/kg/min • 4 周龄 • sc, osmotic pump implanted subcutaneously
    Note
    造模成功指标
    指标变化：WT小鼠血压明显升高。
    外观监测：心脏肥大和纤维化。
    相关产品：/
    拮抗产品：Eplerenone (HY-B0251)

    3. 腹主动脉瘤模型[11]
    致病原理
    Angiotensin II 通过刺激单核细胞募集、巨噬细胞激活以及增强氧化应激来引起动脉粥样硬化，最终导致小鼠腹主动脉局部扩张、变薄，形成腹主动脉瘤。
    具体造模方法
    小鼠：ApoE–/– 小鼠 • 雌性 • 6 月龄
    给药方式：500 或 1000 ng/min/kg • 皮下 • 28 天
    造模成功指标
    细胞变化：增加主动脉内膜的载脂巨噬细胞和淋巴细胞。
    表型观测：存在大量动脉粥样硬化病变。主动脉腹部区域尺寸显著增大，存在大量外膜组织，并且外膜组织内存在血凝块，外表面出现新生血管出现。
    同类产品 /
    拮抗产品(+/-)-硫酸氢氯吡格雷 (HY-107867)

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    1106.23

    Formula

    C52H75N13O14
    CAS 号
    性状

    固体

    颜色

    White to off-white

    Sequence

    Asp-Arg-Val-Tyr-Ile-His-Pro-Phe
    Sequence Shortening

    DRVYIHPF
    中文名称

    血管紧张素 II 醋酸盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light, under nitrogen)

    溶解性数据
    细胞实验： 

    H2O 中的溶解度 : 5 mg/mL (4.52 mM; ultrasonic and warming and heat to 37°C)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 0.9040 mL 4.5199 mL 9.0397 mL
    5 mM --- --- ---
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂： PBS

      Solubility: 10 mg/mL (9.04 mM); 澄清溶液; 超声助溶

    • 方案 二

      请依序添加每种溶剂： Saline

      Solubility: 12.5 mg/mL (11.30 mM); 澄清溶液; 超声助溶 (<60°C)

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.81%

    COA (295 KB) LCMS (90 KB)

    产品使用指南 (1538 KB)
    参考文献

    Angiotensin II human acetate 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light, under nitrogen)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 0.9040 mL 4.5199 mL 9.0397 mL 22.5993 mL

    * 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Angiotensin II human68521-88-0Angiotensin IIAng IIDRVYIHPF血管紧张素 II 醋酸盐Angiotensin ReceptorApoptosisInhibitorinhibitorinhibit

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