1. Neuronal Signaling Metabolic Enzyme/Protease
  2. Monoamine Oxidase Endogenous Metabolite
  3. β-Aminopropionitrile

β-Aminopropionitrile  (Synonyms: BAPN)

目录号: HY-Y1750 纯度: 99.94%
COA 产品使用指南

β-Aminopropionitrile (BAPN) 是一种特异的、不可逆赖的、具有口服活性的氨酰氧化酶 (LOX) 抑制剂。β-Aminopropionitrile 靶向 LOX 或 LOXL 同工酶的活性位点。

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β-Aminopropionitrile Chemical Structure

β-Aminopropionitrile Chemical Structure

CAS No. : 151-18-8

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥610
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500 mg ¥550
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5 g ¥2810
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20 g ¥6100
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50 g   询价  

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Other Forms of β-Aminopropionitrile:

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查看 Monoamine Oxidase 亚型特异性产品:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

β-Aminopropionitrile (BAPN) is a specific, irreversible and orally active lysyl oxidase (LOX) inhibitor. β-Aminopropionitrile targets the active site of LOX or LOXL isoenzymes[1][2].

IC50 & Target

Lysyl Oxidase

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

β-Aminopropionitrile (BAPN) normalizes the expression of GLUT4 and adiponectin, and improves glucose uptake in an in vitro model of insulin resistance[1].
β-Aminopropionitrile (500 μM; 72 h) blocks the hypoxia-induced EMT morphological and marker protein changes, and inhibits invasion and migration capacities of cervical carcinoma cells in vitro[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 200 μM with 1.15 nM and 2.87 nM TNFα
Incubation Time: 72 h
Result: TNFα reduced expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented.

Cell Invasion Assay[2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Significantly reduced hypoxia-elicited cell invasion in both cell models.

Cell Migration Assay [2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Decreased hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively.

Western Blot Analysis[2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin.
体内研究
(In Vivo)

β-Aminopropionitrile (BAPN) (100 mg/kg/day; p.o.; 6 weeks) reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats[1].
β-Aminopropionitrile monofumarate (1 g/kg/day; p.o.; 4 weeks) induces thoracic aortic dissection in C57BL/6 mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats of 150 g, high-fat diet (HFD) model[1]
Dosage: 100 mg/kg/day
Administration: In the drinking water, 6 weeks
Result: Significantly prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. Reduced the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently reduced HOMA index in the HFD group. Improved insulin signalling in adipose tissue from obese animals.
Animal Model: C57BL/6 mice[3]
Dosage: 1 g/kg/day
Administration: In the drinking water, 4 weeks
Result: Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II.
分子量

70.09

Formula

C3H6N2

CAS 号
性状

液体

颜色

Colorless to light yellow

结构分类
初始来源
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 100 mg/mL (1426.74 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : 50 mg/mL (713.37 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 14.2674 mL 71.3369 mL 142.6737 mL
5 mM 2.8535 mL 14.2674 mL 28.5347 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL (46.37 mM); 澄清溶液

    此方案可获得 ≥ 3.25 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;再向上述体系中加入 50 μL Tween-80,混合均匀;然后再继续加入 450 μL 生理盐水 定容至 1 mL

    生理盐水的配制:将 0.9 g 氯化钠,溶解于 ddH₂O 并定容至 100 mL,可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.25 mg/mL (46.37 mM); 澄清溶液

    此方案可获得 ≥ 3.25 mg/mL(饱和度未知)的澄清溶液。

    1 mL 工作液为例,取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中,混合均匀。

    20% SBE-β-CD in Saline 的配制(4°C,储存一周):2 g SBE-β-CD(磺丁基醚 β-环糊精)粉末定容于 10 mL 的生理盐水中,完全溶解至澄清透明。

以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂: PBS

    Solubility: 100 mg/mL (1426.74 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.99%

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 14.2674 mL 71.3369 mL 142.6737 mL 356.6843 mL
5 mM 2.8535 mL 14.2674 mL 28.5347 mL 71.3369 mL
10 mM 1.4267 mL 7.1337 mL 14.2674 mL 35.6684 mL
15 mM 0.9512 mL 4.7558 mL 9.5116 mL 23.7790 mL
20 mM 0.7134 mL 3.5668 mL 7.1337 mL 17.8342 mL
25 mM 0.5707 mL 2.8535 mL 5.7069 mL 14.2674 mL
30 mM 0.4756 mL 2.3779 mL 4.7558 mL 11.8895 mL
40 mM 0.3567 mL 1.7834 mL 3.5668 mL 8.9171 mL
50 mM 0.2853 mL 1.4267 mL 2.8535 mL 7.1337 mL
60 mM 0.2378 mL 1.1889 mL 2.3779 mL 5.9447 mL
80 mM 0.1783 mL 0.8917 mL 1.7834 mL 4.4586 mL
100 mM 0.1427 mL 0.7134 mL 1.4267 mL 3.5668 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
β-Aminopropionitrile
目录号:
HY-Y1750
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