Estradiol (Synonyms: 雌二醇; β-Estradiol; E2; 17β-Estradiol; 17β-Oestradiol)

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Estradiol (β-Estradiol) 是一种类固醇激素和主要的女性性激素。Estradiol 可上调人子宫内膜干细胞 (hEnSCs) 神经标志物的表达并促进其神经分化。Estradiol 可用于癌症、神经退行性疾病和神经组织工程的相关研究。

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CAS No. : 50-28-2

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10 mM * 1 mL in DMSO	￥500	In-stock
500 mg	￥400	In-stock
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Other Forms of Estradiol:

MCE 顾客使用本产品发表的 112 篇科研文献

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RT-PCR

: Estradiol purchased from MCE. Usage Cited in: J Cachexia Sarcopenia Muscle. 2025 Feb;16(1):e13693. [Abstract]; Estradiol (E2) (10-100 nM) exhibites an increase in the SIRT1 protein level in C2C12 cells, without significantly affecting the mRNA level.

: Estradiol purchased from MCE. Usage Cited in: Sci Total Environ. 2024 Jul 6:947:174543. [Abstract]; Estradiol (E2) (1 μM, 8 h) alone or in combination with TEB (100 μM, 8 h) (TEB + E2) significantly increases the protein expression levels of FoxL2 and pFoxL2 (Ser238) in ZF4 cells.

: Estradiol purchased from MCE. Usage Cited in: Aging Cell. 2024 Aug 7:e14303. [Abstract]; Estradiol (3-25 ng/mouse; s.c.; for 7 consecutive days) significantly decreases LAMIN A levels while increasing PROGERIN and PRELAMIN A levels in the uteri of mice injected with 10 ng or 25 ng, compared to those receiving the 3 ng dose.

: Estradiol purchased from MCE. Usage Cited in: Int J Biol Macromol. 2023 May 1:236:123987. [Abstract]; Estradiol (1-100 nM; 6-72 h) promotes the proliferation of sheep myoblasts.

: Estradiol purchased from MCE. Usage Cited in: Theranostics. 2020 Aug 29;10(24):10874-10891. [Abstract]; Estradiol (E2) (100 nM; 36 h) treatment can enhance the expression of ERRα and VLDL-related genes such as Apob, Mttp and Pla2g12b, whereas the induction is blocked with loss of ERRα in primary hepatocytes.

: Estradiol purchased from MCE. Usage Cited in: Environ Pollut. 2021 Feb 1;270:116015. [Abstract]; Estradiol (1-10 nM; 24 h) significantly induces mRNA expression of both pS2 and NRF1 genes in MCF-7 cells.

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生物活性
纯度 & 产品资料
参考文献

生物活性

Estradiol (β-Estradiol) is a steroid hormone and the major female sex hormone. Estradiol can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering^[1]^[2].

IC₅₀ & Target

Human Endogenous Metabolite

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	39.81 μM Compound: ES	Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay	[PMID: 25222876]
CHO	EC₅₀	38.4 μM Compound: 55	Agonist activity at human TGR5 expressed in CHO cells by luciferase assay Agonist activity at human TGR5 expressed in CHO cells by luciferase assay	[PMID: 18307294]
CHO	IC₅₀	> 100 μM Compound: ES	Antiproliferative activity against CHO cells after 2 days by MTT assay Antiproliferative activity against CHO cells after 2 days by MTT assay	[PMID: 25222876]
CHO-K1	EC₅₀	0.03 μM Compound: E2	Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay	[PMID: 20553023]
CHO-K1	EC₅₀	0.036 nM Compound: E2	Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22647217]
CHO-K1	EC₅₀	0.05 nM Compound: E2	Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22647217]
CHO-K1	EC₅₀	0.05 μM Compound: E2	Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay	[PMID: 20553023]
COS-1	EC₅₀	2.4 nM Compound: 1	In vitro agonist activity for estrogen receptor alpha expressed in COS-1 cells In vitro agonist activity for estrogen receptor alpha expressed in COS-1 cells	[PMID: 15713407]
COS-1	EC₅₀	9.7 nM Compound: 1	In vitro agonist activity for estrogen receptor beta expressed in COS-1 cells In vitro agonist activity for estrogen receptor beta expressed in COS-1 cells	[PMID: 15713407]
COS-7	EC₅₀	0.3 nM Compound: 17beta-estradiol	Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry	[PMID: 16520733]
COS-7	EC₅₀	11 nM Compound: Estradiol	Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay	[PMID: 19863083]
COS-7	EC₅₀	3.2 nM Compound: Estradiol	Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay	[PMID: 19863083]
HEC-1	EC₅₀	0.09 nM Compound: Estradiol	Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 21481497]
HEC-1	EC₅₀	0.1 nM Compound: estradiol	Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	[PMID: 19286283]
HEC-1	EC₅₀	0.123 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
HEC-1	EC₅₀	0.13 nM Compound: estradiol	Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 19128016]
HEC-1	EC₅₀	0.16 nM Compound: estradiol	Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay	[PMID: 25559213]
HEC-1	EC₅₀	0.2 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
HEC-1	EC₅₀	0.38 nM Compound: estradiol	Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay	[PMID: 25559213]
HEC-1	EC₅₀	0.5 nM Compound: estradiol	Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay	[PMID: 19286283]
HEC-1	EC₅₀	0.7 nM Compound: estradiol	Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 19128016]
HEC-1	EC₅₀	0.72 nM Compound: Estradiol	Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay	[PMID: 21481497]
HEK-293T	EC₅₀	10.4 nM Compound: E2	Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 20659801]
HEK-293T	EC₅₀	2.5 nM Compound: E2	Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay	[PMID: 20659801]
HEK293	EC₅₀	0.024 nM Compound: beta-Est	Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HEK293	EC₅₀	0.046 nM Compound: 1	Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay	[PMID: 21885279]
HEK293	EC₅₀	0.085 nM Compound: beta-Est	Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay	[PMID: 25305688]
HEK293	EC₅₀	0.09 nM Compound: E2	Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	0.13 nM Compound: E2	Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay	[PMID: 30940565]
HEK293	EC₅₀	0.75 nM Compound: 17-beta-estradiol	Effect on transactivation of ALP gene expression in HEK293 cells transfected with hERalpha Effect on transactivation of ALP gene expression in HEK293 cells transfected with hERalpha	[PMID: 16309907]
HEK293	EC₅₀	0.75 nM Compound: estradiol	Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay	[PMID: 17448656]
HEK293	EC₅₀	0.75 nM Compound: estradiol	Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay	[PMID: 17890084]
HEK293	EC₅₀	2.1 nM Compound: 17-beta-estradiol	Effect on transactivation of ALP gene expression in HEK293 cells transfected with hERbeta Effect on transactivation of ALP gene expression in HEK293 cells transfected with hERbeta	[PMID: 16309907]
HEK293	EC₅₀	2.1 nM Compound: estradiol	Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay	[PMID: 17448656]
HEK293	EC₅₀	2.1 nM Compound: estradiol	Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay	[PMID: 17890084]
HEK293	EC₅₀	4.6 nM Compound: E2	Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay	[PMID: 21218783]
HEK293	IC₅₀	2.88 μM Compound: beta-Estradiol	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT3-expressing HEK293 cells	[PMID: 12110607]
HEK293	IC₅₀	5.73 μM Compound: beta-Estradiol	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT1-expressing HEK293 cells	[PMID: 12110607]
HEK293	IC₅₀	50 μM Compound: Estradiol	Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit	[PMID: 22761000]
HEK293	IC₅₀	> 30 μM Compound: beta-Estradiol	TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells TP_TRANSPORTER: inhibition of MPP+ uptake (MPP+: 0.25 uM) in OCT2-expressing HEK293 cells	[PMID: 12110607]
HRPE	IC₅₀	1.1 μM Compound: beta-Estradiol	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT3-expressing HRPE cells	[PMID: 9830022]
HRPE	IC₅₀	84.8 μM Compound: beta-Estradiol	TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells TP_TRANSPORTER: inhibition of TEA uptake (TEA: 20 uM) in OCT2-expressing HRPE cells	[PMID: 9830022]
HeLa	EC₅₀	0.02 nM Compound: 17-beta-estradiol	Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor alpha. Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor alpha.	[PMID: 11906280]
HeLa	EC₅₀	0.07 nM Compound: 17-beta-estradiol	Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor beta. Activation of estrogen response element in HeLa cells stably transfected with human Estrogen receptor beta.	[PMID: 11906280]
HeLa	EC₅₀	0.21 nM Compound: E2	Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	0.37 nM Compound: E2	Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay	[PMID: 31879182]
HeLa	EC₅₀	0.59 nM Compound: (+)-3,17-beta-estradiol	Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay at 10 uM Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay at 10 uM	[PMID: 16610787]
HeLa	EC₅₀	0.7 nM Compound: 38	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 37077378]
HeLa	EC₅₀	0.84 nM Compound: (+)-3,17-beta-estradiol	Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay Inhibition of ER beta-mediated transactivation of ERE in HeLa cell luciferase assay	[PMID: 16610787]
HeLa	IC₅₀	24 nM Compound: 1	Inhibition of [3H]estradiol binding to human estrogen receptor beta expressed in HeLa cells Inhibition of [3H]estradiol binding to human estrogen receptor beta expressed in HeLa cells	[PMID: 15658851]
HeLa	IC₅₀	28 nM Compound: 1	Inhibition of [3H]estradiol binding to human estrogen receptor alpha expressed in HeLa cells Inhibition of [3H]estradiol binding to human estrogen receptor alpha expressed in HeLa cells	[PMID: 15658851]
HepG2	EC₅₀	11 nM Compound: E2, estradiol	Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22283328]
HepG2	EC₅₀	2.2 nM Compound: E2, estradiol	Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay	[PMID: 22283328]
Hepatocyte	EC₅₀	1.9 μM Compound: Estradiol	Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method	[PMID: 22815312]
Hepatocyte	EC₅₀	4.1 μM Compound: Estradiol	Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method	[PMID: 22815312]
Ishikawa	EC₅₀	0.01 nM Compound: 1	Concentration of compound required to induce 50 % of the maximum stimulation of alkaline phosphatase activity in Ishikawa cells Concentration of compound required to induce 50 % of the maximum stimulation of alkaline phosphatase activity in Ishikawa cells	[PMID: 8201587]
Ishikawa	EC₅₀	0.047 nM Compound: 17-beta-estradiol	Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs	[PMID: 15568770]
Ishikawa	EC₅₀	0.9 nM Compound: E2	Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate	[PMID: 22178552]
Ishikawa	EC₅₀	24.6 pM Compound: E2	Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader	[PMID: 22464681]
JEG-3	IC₅₀	84000 nM Compound: 17-a Estradiol	Inhibition of steroid sulfatase activity of JEG-3 cells Inhibition of steroid sulfatase activity of JEG-3 cells	[PMID: 11087571]
JEG-3	IC₅₀	84000 nM Compound: 3	Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S	[PMID: 9873454]
MCF7	EC₅₀	0.007 nM Compound: 17-beta-estradiol	Agonist effect on transcriptional activation in MCF-7 cells expressing estrogen receptor alpha Agonist effect on transcriptional activation in MCF-7 cells expressing estrogen receptor alpha	[PMID: 10673099]
MCF7	EC₅₀	0.0084 nM Compound: estradiol	Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR	[PMID: 25559213]
MCF7	EC₅₀	0.01 nM Compound: 1	Effect on ERalpha in MCF7 cell line transfected with ER responsive luciferase reporter Effect on ERalpha in MCF7 cell line transfected with ER responsive luciferase reporter	[PMID: 16219463]
MCF7	EC₅₀	0.01 nM Compound: 1 (17-beta-estradiol)	Effective concentration against MCF-7 breast tumor cells using MCF-7 assay. Effective concentration against MCF-7 breast tumor cells using MCF-7 assay.	[PMID: 12825935]
MCF7	EC₅₀	0.01 nM Compound: 4 (estradiol (E2))	Percent agonistic activity for estrogen-induced pS2 expression in MCF-7 cells Percent agonistic activity for estrogen-induced pS2 expression in MCF-7 cells	[PMID: 12749898]
MCF7	EC₅₀	0.02 nM Compound: E2	Effective dose for [3H]- estradiol against proliferation of MCF-7 cells Effective dose for [3H]- estradiol against proliferation of MCF-7 cells	[PMID: 2769681]
MCF7	EC₅₀	0.292 nM Compound: estradiol	Induction of pS2 mRNA expression in human MCF-7 mammary carcinoma cells Induction of pS2 mRNA expression in human MCF-7 mammary carcinoma cells	[PMID: 8627615]
MCF7	EC₅₀	0.3 nM Compound: estradiol	Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR	[PMID: 25559213]
MCF7	EC₅₀	0.7 nM Compound: estradiol	Stimulation of MCF-7 cell proliferation Stimulation of MCF-7 cell proliferation	[PMID: 8941399]
MCF7	EC₅₀	1.52 x 10^-9 M Compound: 5	Growth response in cultures of MCF-7 (human breast cancer cell line) cells Growth response in cultures of MCF-7 (human breast cancer cell line) cells	[PMID: 9357533]
MCF7	EC₅₀	10 nM Compound: E2	Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay	[PMID: 21218783]
MCF7	EC₅₀	3.01 x 10^-11 M Compound: estradiol	Induction of pS2 Gene expression in human MCF-7 cells Induction of pS2 Gene expression in human MCF-7 cells	[PMID: 9371241]
MCF7	EC₅₀	3.7 x 10^-12 M Compound: estradiol	Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay	[PMID: 18760603]
MCF7	EC₅₀	6.4 pM Compound: E2	Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay	[PMID: 22464681]
MCF7	EC₅₀	9.96 x 10^-10 M Compound: estradiol	Induction of pS2 mRNA expression in human MCF-7 mammary carcinoma cells Induction of pS2 mRNA expression in human MCF-7 mammary carcinoma cells	[PMID: 8627615]
MCF7	EC₅₀	8.0 x 10^-11 M Compound: estradiol	Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay	[PMID: 16190777]
MCF7	EC₅₀	0.996 x 10^-10 M Compound: estradiol	Affinity estrogen receptor of MCF-7 human mammary cancer cells Affinity estrogen receptor of MCF-7 human mammary cancer cells	[PMID: 8627615]
MCF7	EC₅₀	1.8 x 10^-10 M Compound: estradiol	Affinity for estrogen receptor of human MCF-7 cells Affinity for estrogen receptor of human MCF-7 cells	[PMID: 9371241]
MCF7	ED₅₀	0.00456 nM Compound: 2	Functional ERalpha receptor activity in transcriptional activation assay in MCF-7 cells Functional ERalpha receptor activity in transcriptional activation assay in MCF-7 cells	[PMID: 12824043]
MCF7	IC₅₀	0.32 nM Compound: estradiol	Effect on estradiol-stimulated MCF-7 cell proliferation Effect on estradiol-stimulated MCF-7 cell proliferation	[PMID: 8941399]
MCF7	IC₅₀	0.4 nM Compound: estrogen	Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay	[PMID: 16520733]
MCF7	IC₅₀	27.35 μM Compound: ES	Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay	[PMID: 25222876]
MCF7	IC₅₀	3 nM Compound: estradiol	Displacement of [3H]estradiol from Estrogen receptor in MCF-7 cells Displacement of [3H]estradiol from Estrogen receptor in MCF-7 cells	[PMID: 1548683]
MCF7	IC₅₀	32 μM Compound: estradiol	Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay	[PMID: 12565971]
MDA-MB-231	IC₅₀	33.46 μM Compound: ES	Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay	[PMID: 25222876]
MDA-MB-231	IC₅₀	> 100 μM Compound: estradiol	Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay	[PMID: 12565971]
MIA PaCa-2	IC₅₀	42.93 μM Compound: ES	Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay	[PMID: 25222876]
NIH3T3	IC₅₀	> 100 μM Compound: ES	Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay	[PMID: 25222876]
SAOS-2	ED₅₀	4.7 nM Compound: 2	Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells	[PMID: 12824043]
SH-SY5Y	EC₅₀	0.1 nM Compound: 17-beta-estradiol	Agonist activity in transcriptional activation assay in SH-SY5Y neuroblastoma cells expressing Estrogen receptor beta Agonist activity in transcriptional activation assay in SH-SY5Y neuroblastoma cells expressing Estrogen receptor beta	[PMID: 11906280]
SK-BR-3	IC₅₀	0.1 nM Compound: estrogen	Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay	[PMID: 16520733]
Sf9	IC₅₀	12.5 nM Compound: E2	Displacement of [3H]17beta-estradiol from human ERalpha expressed in SF9 cells Displacement of [3H]17beta-estradiol from human ERalpha expressed in SF9 cells	[PMID: 19836949]
T47D	EC₅₀	0.07 nM Compound: 17-beta-E2	Agonist effect on transcriptional activation in T47D cells expressing estrogen receptor alpha Agonist effect on transcriptional activation in T47D cells expressing estrogen receptor alpha	[PMID: 12459017]
T47D	EC₅₀	0.8 nM Compound: 17-beta-E2	Agonist effect on transcriptional activation of T47D cells expressing human estrogen receptor beta Agonist effect on transcriptional activation of T47D cells expressing human estrogen receptor beta	[PMID: 12459017]
U2OS	EC₅₀	0.004 nM Compound: E2	Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control	[PMID: 23043242]
U2OS	EC₅₀	0.01 nM Compound: E2	Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control	[PMID: 23043242]
U2OS	EC₅₀	0.019 nM Compound: estradiol	Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT	[PMID: 16162002]
U2OS	EC₅₀	0.098 nM Compound: estradiol	Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT	[PMID: 16162002]
U2OS	EC₅₀	0.15 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]
U2OS	EC₅₀	0.4 nM Compound: E2, 17beta-E2, estradiol	Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay	[PMID: 22122563]

体外研究
(In Vitro)

Estradiol (10 nM，7 d) 诱导人子宫内膜干细胞 (hEnSCs) 的神经分化和增加轴突分支^[1]。
Estradiol (17β-estradiol，10 nM，7 days) 增加 hEnSCs 分化的神经样细胞中神经元样细胞标志物 (Tuj-1、巢蛋白和 NF-H) 的表达^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the number of neurite processes including neural differentiation and neurite branching.

Immunofluorescence^[1]

Cell Line:	Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration:	10 nM
Incubation Time:	7 days
Result:	Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.

体内研究
(In Vivo)

Estradiol (1 nM，来自 FBN-ARO-KO 小鼠的海马切片) 挽救长期增强 (LTP) 振幅^[1]。
Estradiol (0.0167 mg，皮下植入，FBN-ARO-KO 小鼠) 挽救 FBN-ARO-KO 小鼠的分子和功能缺陷^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	1 nM
Administration:	Treated for the hippocampal slices
Result:	Rescued long-term potentiation (LTP) amplitude of both male and female mice.

Animal Model:	FBN-ARO-KO Mice^[2]
Dosage:	0.0167 mg
Administration:	Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation.
Result:	Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain. Rescued the spatial learning and memory defects.

Clinical Trial

分子量

272.38

Formula

C₁₈H₂₄O₂

CAS 号

50-28-2

性状

固体

颜色

White to off-white

中文名称

雌二醇；强力求偶素

结构分类

Steroids

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

溶解性数据

细胞实验：

DMSO 中的溶解度 : 100 mg/mL (367.13 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

Ethanol 中的溶解度 : 16.67 mg/mL (61.20 mM; 超声助溶)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6713 mL	18.3567 mL	36.7134 mL
5 mM	0.7343 mL	3.6713 mL	7.3427 mL
10 mM	0.3671 mL	1.8357 mL	3.6713 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: 2.5 mg/mL (9.18 mM); 悬浊液; 超声助溶
方案三

请依序添加每种溶剂： 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液
方案四

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.64 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案五

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.64 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： Corn Oil
Solubility: 12.5 mg/mL (45.89 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.93%

选择批次：

Data Sheet (643 KB) SDS (623 KB)

COA (286 KB) HNMR (280 KB) LCMS (87 KB) 元素分析报告 (210 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Elham Hasanzadeh. Defining the role of 17β-estradiol in human endometrial stem cells differentiation into neuron-like cells. Cell Biol Int. 2021 Jan;45(1):140-153. [Content Brief]

[2]. Yujiao Lu, et al. Neuron-Derived Estrogen Regulates Synaptic Plasticity and Memory. J Neurosci. 2019 Apr 10;39(15):2792-2809. [Content Brief]

Estradiol 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.6713 mL	18.3567 mL	36.7134 mL	91.7835 mL
	5 mM	0.7343 mL	3.6713 mL	7.3427 mL	18.3567 mL
	10 mM	0.3671 mL	1.8357 mL	3.6713 mL	9.1784 mL
	15 mM	0.2448 mL	1.2238 mL	2.4476 mL	6.1189 mL
	20 mM	0.1836 mL	0.9178 mL	1.8357 mL	4.5892 mL
	25 mM	0.1469 mL	0.7343 mL	1.4685 mL	3.6713 mL
	30 mM	0.1224 mL	0.6119 mL	1.2238 mL	3.0595 mL
	40 mM	0.0918 mL	0.4589 mL	0.9178 mL	2.2946 mL
	50 mM	0.0734 mL	0.3671 mL	0.7343 mL	1.8357 mL
	60 mM	0.0612 mL	0.3059 mL	0.6119 mL	1.5297 mL
DMSO	80 mM	0.0459 mL	0.2295 mL	0.4589 mL	1.1473 mL
DMSO	100 mM	0.0367 mL	0.1836 mL	0.3671 mL	0.9178 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Estradiol (Synonyms: 雌二醇; β-Estradiol; E2; 17β-Estradiol; 17β-Oestradiol)

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Other Forms of Estradiol:

MCE 顾客使用本产品发表的 112 篇科研文献

Estradiol 相关产品

•相关化合物库:

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Estradiol (Synonyms: 雌二醇; β-Estradiol; E2; 17β-Estradiol; 17β-Oestradiol)

Customer Review

Other Forms of Estradiol:

Estradiol 相关抗体

MCE 顾客使用本产品发表的 112 篇科研文献

Estradiol 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Estrogen Receptor/ERR 亚型特异性产品:

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参考文献

Estradiol 相关抗体:

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