BIHC 

BIHC is a TNF blocker with anti-inflammatory activity. BIHC can significantly inhibit the proliferation of hepatocellular carcinoma (HCC) cells and exhibits potent cytotoxicity against the HepG2 cell line, capable of inducing cell apoptosis , while demonstrating relatively low toxicity towards normal hepatocytes. Additionally, BIHC can be used for research on inflammatory bowel disease (IBD)^[1].

BIHC (5-25 μM; 24-72 h) 可显著抑制肝细胞癌 (HCC) 细胞增殖，其体外抗 HepG2 细胞活性的 IC₅₀ 值为 6.4 μM，并能在体外抑制 TNF-α 与其抗体的结合，IC₅₀ 为 16.5 μM^[1]。
BIHC (25 μM; 24 -72 h) 在 HepG2 细胞中通过破坏 TNF 三聚体结构，抑制 NF-κB 信号通路及其下游基因产物 (如 cyclin D1、Mcl-1、survivin、Bcl-2)，从而诱导肝癌细胞凋亡^[1]。
BIHC (25 μM; 8 h) 还可以显著抑制 CXCL12 (20 μM; 2 h) 诱导的 HepG2 细胞侵袭^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

BIHC (5 mg/kg; i.p.,Once daily for 5 days) 可显著缓解葡聚糖硫酸钠 (DSS) 诱导的 Swiss albino 小鼠结肠炎，降低促炎因子 TNF-α、IFN-γ、IL-1β、IL-6 水平，同时上调抗炎因子 IL-10^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

492.91

C₂₆H₂₁ClN₂O₆

1642826-40-1

固体

Off-white to light yellow

Room temperature in continental US; may vary elsewhere.

• [1]. Keerthy H K, Mohan C D, Siveen K S, et al. Novel synthetic biscoumarins target tumor necrosis factor-α in hepatocellular carcinoma in vitro and in vivo[J]. Journal of Biological Chemistry, 2014, 289(46): 31879-31890.  [Content Brief]