2. Stem Cell/Wnt Cell Cycle/DNA Damage
  3. β-catenin Wnt CDK
  4. β-catenin-IN-3

β-catenin-IN-3 (Compound C2) 是一种选择性 β-catenin 抑制剂。β-catenin-IN-3 与 β-catenin 表面的新型变构位点结合， KD 值为 54.96 nM。 β-catenin-IN-3 通过靶向隐蔽的变构调节位点选择性地抑制 β-catenin， 降低其细胞负荷。 β-catenin-IN-3 显著降低 β-catenin 驱动的癌细胞的活力，在 β-catenin 过表达癌细胞中通过蛋白酶体系统触发其降解。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

β-catenin-IN-3

β-catenin-IN-3 Chemical Structure

CAS No. : 1005288-15-2

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥2000
In-stock
5 mg ￥5200
In-stock
10 mg 现货 询价
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Customer Review

β-catenin-IN-3 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 Wnt 亚型特异性产品:

查看所有亚型
Wnt1 Wnt3 Wnt5 Wnt7

查看 CDK 亚型特异性产品:

查看所有亚型
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

β-catenin-IN-3 (Compound C2) is a selective β-catenin inhibitor. β-catenin-IN-3 binds to allosteric site on the surface of β-catenin with K D calculated at 54.96 nM. β-catenin-IN-3 selectively inhibits β-catenin via targeting a cryptic allosteric modulation site, lowers its cellular load. β-catenin-IN-3 significantly reduces viability of β-catenin driven cancer cells, and triggers β-catenin degradation via proteasome system in β-catenin-overexpressing cancer cells[1].

IC50 & Target[1]

Cdk4/cyclin D1

 

细胞效力
(Cellular Effect)
Cell Line Type Value Description References
SW480 IC50
1.35 μM
Compound: 65; C2
Antiproliferative activity against human SW480 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
Antiproliferative activity against human SW480 cells assessed as inhibition of cell growth incubated for 72 hrs by alamar blue assay
[PMID: 36183504]
体外研究
(In Vitro)

β-catenin-IN-3 (24 h)以剂量依赖性方式降低 DLD1，SW480，HCT116，SW48，MCF10A 和 H460 细胞的活力，IC50s为 0.85 μM、1.35 μM、5.35 μM、3.45 μM 和 20 μM 以上。[1]。
β-catenin-IN-3 (1 μM，3 μM，7 天) 抑制 DLD1 和 SW48 细胞的集落形成能力。[1]。
β-catenin-IN-3 (0，1，5 μM，24 h) 以剂量依赖的方式选择性抑制 DLD1 和 SW480 细胞中 β-catenin、 Axin1、 CyclinD1 和 TCF4 的水平[1]。
β-catenin-IN-3 (1 μM，3 h) 在 DLD1 细胞中通过泛素-蛋白酶体机制触发 β-catenin 的降解，可能是通过募集 APC 介导的破坏复合物[1]。
β-catenin-IN-3 (1 μM，1-24 h) 增加 DLD1 细胞中 β-catenin 的磷酸化[1] 。
β-catenin-IN-3 (1，5 μM，24 h) 分别降低了细胞核和细胞质中的 β-catenin 45%，40%，85% 和 55%[1]。
β-catenin-IN-3 (3，5 μM， 4 天) 抑制 APC 缺陷类器官克隆形成能力约 70% 和 55%，相反，野生型类器官的存活率为90%[1] 。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

β-catenin-IN-3 相关抗体:

Cell Viability Assay[1]

Cell Line: DLD1,SW4808, HCT116, SW48, MCF10A, H460 cells
Concentration: 10 nM-100 μM
Incubation Time: 24 h
Result: Reduced viability of DLD1 and SW480 cells in dose-dependent manner with IC50s of 0.85 μM and 1.35 μM.
Reduced viability of HCT116 and SW48 cells in dose-dependent manner with IC50s of 5.35 μM and 3.45 μM.
Impacted two WNT-independent cell lines, MCF10A and H460, with IC50 above 20μM.

Western Blot Analysis[1]

Cell Line: DLD1 cells
Concentration: 1μM
Incubation Time: 1,3,6,12,24 h
Result: Phosphorylated β-catenin starting at 1 hour point, which was further amplified to 2-2.5-fold at 12 and 24 hour points, and can be blocked by CHIR99021 (HY-10182).
Levels of active β-catenin markedly reduced at these time points, and can be arrested by MG132 (HY-13259).

Immunofluorescence[1]

Cell Line: DLD1 cells
Concentration: 1 μM
Incubation Time: 24 h
Result: Reduced β-catenin that co-localized with nucleic acids inside the nucleus by 55-60% (co-localization dropped from 0.62 to 0.25).
体内研究
(In Vivo)
分子量

484.25

Formula

C19H20Br2N2OS
CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

纯度 & 产品资料
参考文献

β-catenin-IN-3 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-catenin-IN-31005288-15-2β-cateninWntCDKBeta cateninCyclin dependent kinaseAnticancerWnt pathwayβ-catenin-driven cancer cellstherapeutic targetingInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
β-catenin-IN-3
目录号：
HY-147007
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z