2. Autophagy Immunology/Inflammation Anti-infection
  3. Parasite Autophagy SARS-CoV Toll-like Receptor (TLR) HIV Antibiotic
  4. Chloroquine dihydrochloride

Chloroquine dihydrochloride  (Synonyms: 盐酸氯喹)

目录号: HY-17589B 纯度: 99.61%
COA 产品使用指南 技术支持

Chloroquine dihydrochloride 是一种广泛用于疟疾和类风湿性关节炎的抗疟疾和抗炎剂。Chloroquine dihydrochloride 是autophagy 和toll-like receptors (TLRs) 的抑制剂。Chloroquine dihydrochloride 有效抑制 SARS-CoV-2 (COVID-19) 感染 (EC50=1.13 μM)。

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Chloroquine dihydrochloride Chemical Structure

Chloroquine dihydrochloride Chemical Structure

CAS No. : 3545-67-3

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥385
In-stock
5 mg ￥350
In-stock
10 mg ￥560
In-stock
25 mg ￥1180
In-stock
50 mg ￥1830
In-stock
100 mg ￥2850
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Other Forms of Chloroquine dihydrochloride:

Chloroquine dihydrochloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 758 篇科研文献

IF
WB
Cell Viability Assay
Proliferation Assay

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Mol Cancer. 2024 Apr 29;23(1):86.  [Abstract]

    293 cells transfected with the indicated vectors were treated with chloroquine (20 μM) or DMSO for 6 h. Cell lysates were prepared, and the CDC6 protein level was measured by Western blotting.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: ACS Nano. 2024 Aug 28.  [Abstract]

    ​Chloroquine (50 nM, 24 h), as an autophagy inhibitor, blocks autophagosomal-lysosomal fusion in HT22 cells, leading to impaired autophagic flux.​

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: ACS Nano. 2024 Jan 22.  [Abstract]

    The protein levels of SOX2 in 100 μg/mL of PS-NP-exposed Swan 71 cells treated with CHX (10 μg/mL; 4 h) and MG132 (10 μM; 6 h) or chloroquine (CQ) (15 μM; 6 h).

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: J Extracell Vesicles. 2024 Jul;13(7):e12468.  [Abstract]

    Western blotting showing the protein degradation of HEXB by using Chloroquine (CQ) (10 μM, 24 h).

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Cell Res. 2023 Jul 17.  [Abstract]

    A375 cells were pretreated with CQ (30μM) or MG132 (100 nM) for 2 hours, and then treated with CCCP/FeSO4 plus mannose for 24 hours. Tubulin was used to determine the amount of loading proteins.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Cancer Commun (Lond). 2023 Apr 2.  [Abstract]

    WB analysis of LC3 and p62 expression in MCF7 and SKBR3 cells after YAP1 transient overexpression for 48 h with or without EBSS (6 h) starvation or CQ (Chloroquine) (20 µmol/L, 6 h) treatment.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: ACS Nano. 2023 Nov 15.  [Abstract]

    Sorafenib-sensitive HepG2 cells, with or without URB1-AS1 overexpression, were treated with the lysosomal inhibitor chloroquine or vehicle for 24 h. Ferritin H levels were analyzed by Western blot.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: J Extracell Vesicles. 2023 Jul;12(7):e12342.  [Abstract]

    After pretreating cells with 10μM CQ, the levels of phospho‐AMPKα and autophagic markers in the cells were quantified by immunoblotting using the specific antibodies. β‐actin serves as a loading control.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: J Extracell Vesicles. 2023 Jul;12(7):e12342.  [Abstract]

    The sEV cocultured 22Rv1 cells were pretreated with 1μM CQ and followed by IR treatment. The cell survival rate was determined using colony survival assay (n = 3).

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Immun Inflamm Dis. 2023 Apr 12; 11:e830.

    Chloroquine (10 μM; 30 min) reverses the effect of OmpA on autophagy by inhibiting the expression of Beclin1 and LC3II/I while increasing the expression of P62 in BMDCs.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Immun Inflamm Dis. 2023 Apr 12; 11:e830.

    Chloroquine (10 μM; 30 min) reverses the effect of OmpA on autophagy by inhibiting the expression of LC3 in BMDCs.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Prostate. 2019 Jan;79(1):44-53.  [Abstract]

    Treatment with sub-dose of Everolimus (10 nM), together with CQ (5 µM) increases the levels of Bax and cleaved PARP, and decreases the levels of Bcl-2 expression in both PC3 and LNPER cells.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Oncogene. 2018 May;37(22):2936-2952.  [Abstract]

    IPZ-treated U87 and U251 cells are treated with or without Baf-A1 (5 nM), CQ (40 μM), or MG132 (1 μM) for 48 h, followed by western blots.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Dec;109:1617-1627.  [Abstract]

    GFP-LC3B-expressing LX-2 cells are treated with DMSO, 3-MA (10 mM), or Chloroquine (30 μM) alone or in the presence of Carvedilol (CVD, 10 μM) for 24 h. The conversion of LC3B-I to LC3B-II is evaluated using immunoblotting.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: J Pharmacol Exp Ther. 2018 Oct;367(1):20-27.  [Abstract]

    Protein expression of LC3B and p62 in Panc-1 cells treated with Carboxamidotriazole (CAI) and/or Chloroquine (CQ).

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Sep 5;503(2):501-507.  [Abstract]

    Western blot analysis of autophagy markers and LAMP2 in mice with AAV-vaspin and Chloroquine (CQ) pretreatment after I/R (1d).

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: EBioMedicine. 2017 May;19:49-59.  [Abstract]

    mCherry-GFPLC3 puncta analysis in SPC-A-1 cells. Cells are transfected with mCherry-GFP-LC3 plasmid and incubated for 6 h. Next, cells are pretreated with or without Chloroquine (50 μM) for 1 h followed by stimulation of 5 μM Flu for 24 h or 36 h. Scale bar, 10 μm and 2 μm (magnified graph). The lysosomal inhibitor Chloroquine (CQ) raises the lysosomal pH and increases the autophagic flux.

    Chloroquine dihydrochloride purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2016 Dec;48(12):1075-1084.  [Abstract]

    The change of LC3 is analyzed by western blotting after 6 h hypoxia in the presence or absence of Chloroquine of WDR26-overexpressing H9c2 cells.

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    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Chloroquine dihydrochloride is an antimalarial and anti-inflammatory agent widely used to treat malaria and rheumatoid arthritis. Chloroquine dihydrochloride is an autophagy and toll-like receptors (TLRs) inhibitor. Chloroquine dihydrochloride is highly effective in the control of SARS-CoV-2 (COVID-19) infection in vitro (EC50=1.13 μM)[1][2][3][4].

    IC50 & Target[1][2][3][5]

    Plasmodium

     

    Malaria

     

    TLRs

     

    SARS-COV-2

     

    HIV-1

     

    体外研究
    (In Vitro)

    Chloroquine dihydrochloride (20 μM) inhibits IL-12p70 release and reduces Th1-priming capacity of activated human monocyte-derived Langerhans-like cells (MoLC). Chloroquine dihydrochloride (20 μM) enhances IL-1–induced IL-23 secretion in MoLC and subsequently increases IL-17A release by primed CD4+ T cells[1]. Chloroquine dihydrochloride (25 μM) suppresses MMP-9 mRNA expression in normoxia and hypoxia in parental MDA-MB-231 cells. Chloroquine dihydrochloride has cell-, dose- and hypoxia-dependent effects on MMP-2, MMP-9 and MMP-13 mRNA expression[2]. TLR7 and TLR9 inhibition using IRS-954 or Chloroquine dihydrochloride significantly reduces HuH7 cell proliferation in vitro[3].
    Chloroquine dihydrochloride (0.01-100 μM; 48 hours) potently blocked virus infection (vero E6 cells infected with SARS-CoV-2) at low-micromolar concentration (EC50=1.13 μM). Chloroquine dihydrochloride blocks virus infection by increasing endosomal pH required for virus/cell fusion, as well as interfering with the glycosylation of cellular receptors of SARS-CoV[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Chloroquine dihydrochloride 相关抗体:
    体内研究
    (In Vivo)

    Chloroquine dihydrochloride (80 mg/kg, i.p.) does not prevent the growth of the triple-negative MDA-MB-231 cells with high or low TLR9 expression levels in the orthotopic mouse model[2].
    TLR7 and TLR9 inhibition using IRS-954 or Chloroquine dihydrochloride significantly inhibits tumour growth in the mouse xenograft model. HCC development in the DEN/NMOR rat model is also significantly inhibited by Chloroquine[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    392.79

    同用名

    盐酸氯喹

    Formula

    C18H28Cl3N3
    CAS 号
    性状

    固体

    颜色

    White to yellow

    中文名称

    盐酸氯喹
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : 140 mg/mL (356.42 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 2.5459 mL 12.7294 mL 25.4589 mL
    5 mM 0.5092 mL 2.5459 mL 5.0918 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3.5 mg/mL (8.91 mM); 澄清溶液

      此方案可获得 ≥ 3.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3.5 mg/mL (8.91 mM); 澄清溶液

      此方案可获得 ≥ 3.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 35.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

      2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料
    参考文献

    Chloroquine dihydrochloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5459 mL 12.7294 mL 25.4589 mL 63.6472 mL
    5 mM 0.5092 mL 2.5459 mL 5.0918 mL 12.7294 mL
    10 mM 0.2546 mL 1.2729 mL 2.5459 mL 6.3647 mL
    15 mM 0.1697 mL 0.8486 mL 1.6973 mL 4.2431 mL
    20 mM 0.1273 mL 0.6365 mL 1.2729 mL 3.1824 mL
    25 mM 0.1018 mL 0.5092 mL 1.0184 mL 2.5459 mL
    30 mM 0.0849 mL 0.4243 mL 0.8486 mL 2.1216 mL
    40 mM 0.0636 mL 0.3182 mL 0.6365 mL 1.5912 mL
    50 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
    60 mM 0.0424 mL 0.2122 mL 0.4243 mL 1.0608 mL
    80 mM 0.0318 mL 0.1591 mL 0.3182 mL 0.7956 mL
    100 mM 0.0255 mL 0.1273 mL 0.2546 mL 0.6365 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Chloroquine3545-67-3盐酸氯喹ParasiteAutophagySARS-CoVToll-like Receptor (TLR)HIVAntibioticSARS coronavirusHuman immunodeficiency virusmalariainflammatoryrheumatoidarthritisSARS-CoV-2COVID-19infectionimmune-modulatingInhibitorinhibitorinhibit

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