Flavopiridol (Synonyms: 夫拉平度; HMR-1275; Alvocidib; L86-8275)

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Flavopiridol (Alvocidib) 是一种竞争型的 CDK 广谱抑制剂，抑制 CDK1，CDK2，CDK4的IC₅₀ 分别为30，170，100 nM。

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Flavopiridol Chemical Structure

CAS No. : 146426-40-6

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥371	In-stock
1 mg	￥250	In-stock
5 mg	￥420	In-stock
10 mg	￥640	In-stock
25 mg	￥1280	In-stock
50 mg	￥1920	In-stock
100 mg	￥2885	In-stock
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Other Forms of Flavopiridol:

Flavopiridol Hydrochloride In-stock

MCE 顾客使用本产品发表的 52 篇科研文献

RT-PCR

Proliferation Assay

: Flavopiridol purchased from MCE. Usage Cited in: Sci Rep. 2015 Dec 1;5:17675. [Abstract]; Flavopiridol (FP) significantly decreases renilla luciferase mRNA levels time-dependently. The bar graphs (upper panel) represent renilla luciferase mRNA levels in pRL-SV40-transfected A549 cells treated with FP normalized to that in FP free cells. The bottom panel indicates renilla luciferase mRNA levels determined by RT-PCR using 1 μg of total RNA as template.

: Flavopiridol purchased from MCE. Usage Cited in: Biomaterials. 2014 Aug;35(24):6585-94. [Abstract]; Flavopiridol at concentrations >0.1 μM significantly inhibits the proliferation of astrocytes, and at ≥1 μM even inhibits their survival.

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Flavopiridol (Alvocidib) is a broad spectrum and competitive inhibitor of CDKs, inhibiting CDK1, CDK2, CDK4 with IC₅₀s of 30, 170, 100 nM, respectively.

IC₅₀ & Target^[3]

CDK1/Cyc B1

30 nM (IC₅₀)

CDK2/Cyc E

170 nM (IC₅₀)

CDK4/Cyc D1

100 nM (IC₅₀)

MAP

19000 nM (IC₅₀)

PKC

14000 nM (IC₅₀)

EGFR

22000 nM (IC₅₀)

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	IC₅₀	0.075 μM Compound: Flavopiridol	Inhibition of A431 human squamous cell carcinoma cell proliferation Inhibition of A431 human squamous cell carcinoma cell proliferation	[PMID: 12190313]
A2780	GI₅₀	0.023 μM Compound: Flavopiridol	Cytotoxicity against human A2780 cells after 24 hrs by MTT assay Cytotoxicity against human A2780 cells after 24 hrs by MTT assay	[PMID: 23301767]
A2780	GI₅₀	0.029 μM Compound: Flavopiridol	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 23301767]
A2780	GI₅₀	0.031 μM Compound: Flavopiridol	Cytotoxicity against human A2780 cells after 48 hrs by MTT assay Cytotoxicity against human A2780 cells after 48 hrs by MTT assay	[PMID: 23301767]
A2780	GI₅₀	0.064 μM Compound: Flavopiridol	Growth inhibition of human A2780 cells after 48 hrs by MTT assay Growth inhibition of human A2780 cells after 48 hrs by MTT assay	[PMID: 33581551]
A2780	IC₅₀	0.038 μM Compound: Flavopiridol	Inhibition of A2780 / DDP-R human ovarian carcinoma cell proliferation Inhibition of A2780 / DDP-R human ovarian carcinoma cell proliferation	[PMID: 12190313]
A2780	IC₅₀	0.056 μM Compound: Flavopiridol	Inhibition of A2780 / DDP-S human ovarian carcinoma cell proliferation Inhibition of A2780 / DDP-S human ovarian carcinoma cell proliferation	[PMID: 12190313]
A2780	IC₅₀	0.065 μM Compound: Flavopiridol	Inhibition of A2780 / TAX-S human ovarian carcinoma cell proliferation Inhibition of A2780 / TAX-S human ovarian carcinoma cell proliferation	[PMID: 12190313]
A2780	IC₅₀	0.071 μM Compound: 1 (flavopiridol)	Antiproliferative effect against human A2780 cell line was determined in a whole cell 72 hr cytotoxicity assay Antiproliferative effect against human A2780 cell line was determined in a whole cell 72 hr cytotoxicity assay	[PMID: 15027863]
A2780	IC₅₀	0.078 μM Compound: Flavopiridol	Inhibition of A2780 / TAX-R human ovarian carcinoma cell proliferation Inhibition of A2780 / TAX-R human ovarian carcinoma cell proliferation	[PMID: 12190313]
A2780	IC₅₀	15 μM Compound: 1 (flavopiridol)	Inhibition of A2780 cell clonogenic assay Inhibition of A2780 cell clonogenic assay	[PMID: 11063609]
A2780	IC₅₀	71 nM Compound: 1	Cytotoxic effect on human ovarian (A2780) cancer cell line Cytotoxic effect on human ovarian (A2780) cancer cell line	[PMID: 15125971]
A549	CC₅₀	> 100 μM Compound: 11; L86-8275, HMR1275	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 33539089]
A549	GI₅₀	0.14 μM Compound: FVP	Antiproliferative activity against human A549 cells after 3 days by SRB method Antiproliferative activity against human A549 cells after 3 days by SRB method	[PMID: 25914804]
A549	GI₅₀	0.145 μM Compound: Flavopiridol	Growth inhibition of human A549 cells after 48 hrs by MTT assay Growth inhibition of human A549 cells after 48 hrs by MTT assay	[PMID: 33581551]
A549	IC₅₀	0.096 μM Compound: Flavopiridol	Inhibition of A549 human lung carcinoma cell proliferation Inhibition of A549 human lung carcinoma cell proliferation	[PMID: 12190313]
A549	IC₅₀	0.1 μM Compound: Flavopiridol	Antiproliferative activity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
ABAE	IC₅₀	0.045 μM Compound: Flavopiridol	Inhibition of ABAE human fibroblast cell proliferation Inhibition of ABAE human fibroblast cell proliferation	[PMID: 12190313]
BJ	EC₅₀	> 86.2069 μM Compound: 4	Antiproliferative activity against human BJ cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human BJ cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
CCRF-CEM	IC₅₀	0.052 μM Compound: Flavopiridol	Inhibition of CCRF-CEM human leukemia cell proliferation Inhibition of CCRF-CEM human leukemia cell proliferation	[PMID: 12190313]
CNE-2	IC₅₀	0.12 μM Compound: Flavopiridol	Antiproliferative activity against human CNE-2 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human CNE-2 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
COLO 205	EC₅₀	> 20 μM Compound: Chemical Probe: Flavopiridol	Cell cycle arrest in human COLO 205 cells assessed as accumulation of cells at G1 phase by measuring increase in 2N DNA content measured after 24 hrs by propidium iodide staining based laser-scanning fluorescence cytometry Cell cycle arrest in human COLO 205 cells assessed as accumulation of cells at G1 phase by measuring increase in 2N DNA content measured after 24 hrs by propidium iodide staining based laser-scanning fluorescence cytometry	[PMID: 24919854]
CWR22R	IC₅₀	0.24 μM Compound: FP	Antiproliferative activity against human 22Rv1 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay Antiproliferative activity against human 22Rv1 cells assessed as reduction in cell viability incubated for 72 hrs by SRB assay	[PMID: 37672930]
Caco-2	IC₅₀	0.086 μM Compound: Flavopiridol	Inhibition of CACO-2 human colon carcinoma cell proliferation Inhibition of CACO-2 human colon carcinoma cell proliferation	[PMID: 12190313]
DU-145	GI₅₀	0.15 μM Compound: FVP	Antiproliferative activity against human DU145 cells after 3 days by SRB method Antiproliferative activity against human DU145 cells after 3 days by SRB method	[PMID: 25914804]
HCT-116	EC₅₀	0.034 μM Compound: Flavopiridol	Antiproliferative activity against human HCT116 cells after 72 hrs by Celltiter-Glo reagent based assay in presence of 10% fetal bovine serum Antiproliferative activity against human HCT116 cells after 72 hrs by Celltiter-Glo reagent based assay in presence of 10% fetal bovine serum	[PMID: 26985305]
HCT-116	EC₅₀	0.059 μM Compound: Flavopiridol	Antiproliferative activity against human HCT116 cells after 72 hrs by Celltiter-Glo reagent based assay in presence of 0.625% fetal bovine serum Antiproliferative activity against human HCT116 cells after 72 hrs by Celltiter-Glo reagent based assay in presence of 0.625% fetal bovine serum	[PMID: 26985305]
HCT-116	EC₅₀	0.059 μM Compound: Flavopiridol	Antiproliferative activity against human HCT116 cells assessed as growth inhibition in presence of 0.625% FBS after 72 hrs Antiproliferative activity against human HCT116 cells assessed as growth inhibition in presence of 0.625% FBS after 72 hrs	[PMID: 27326333]
HCT-116	GI₅₀	0.056 μM Compound: Flavopiridol	Growth inhibition of human HCT-116 cells after 48 hrs by MTT assay Growth inhibition of human HCT-116 cells after 48 hrs by MTT assay	[PMID: 33581551]
HCT-116	IC₅₀	0.017 μM Compound: Flavopiridol	Inhibition of HCT116 / VP35 human colon carcinoma cell proliferation Inhibition of HCT116 / VP35 human colon carcinoma cell proliferation	[PMID: 12190313]
HCT-116	IC₅₀	0.018 μM Compound: Flavopiridol	Inhibition of HCT116 human colon carcinoma cell proliferation Inhibition of HCT116 human colon carcinoma cell proliferation	[PMID: 12190313]
HCT-116	IC₅₀	0.021 μM Compound: Flavopiridol	Inhibition of HCT116 / VM46 human colon carcinoma cell proliferation Inhibition of HCT116 / VM46 human colon carcinoma cell proliferation	[PMID: 12190313]
HCT-116	IC₅₀	0.03 μM Compound: Flavopiridol	Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
HCT-116	IC₅₀	13 μM Compound: 1 (flavopiridol)	Inhibition of HCT116 cell clonogenic assay Inhibition of HCT116 cell clonogenic assay	[PMID: 11063609]
HCT-116	IC₅₀	20 nM Compound: Alvocidib	Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
HL-60	IC₅₀	0.046 μM Compound: Flavopiridol	Inhibition of HL60 human leukemia cell proliferation Inhibition of HL60 human leukemia cell proliferation	[PMID: 12190313]
HMEC-1	GI₅₀	0.061 μM Compound: Flavopiridol	Cytotoxicity against human HMEC1 cells after 24 hrs by MTT assay Cytotoxicity against human HMEC1 cells after 24 hrs by MTT assay	[PMID: 23301767]
HMEC-1	GI₅₀	0.062 μM Compound: Flavopiridol	Cytotoxicity against human HMEC1 cells after 48 hrs by MTT assay Cytotoxicity against human HMEC1 cells after 48 hrs by MTT assay	[PMID: 23301767]
HMEC-1	GI₅₀	0.066 μM Compound: Flavopiridol	Cytotoxicity against human HMEC1 cells after 72 hrs by MTT assay Cytotoxicity against human HMEC1 cells after 72 hrs by MTT assay	[PMID: 23301767]
HS27	IC₅₀	0.051 μM Compound: Flavopiridol	Inhibition of Hs 27 human fibroblast cell proliferation Inhibition of Hs 27 human fibroblast cell proliferation	[PMID: 12190313]
HT-29	GI₅₀	0.131 μM Compound: Flavopiridol	Growth inhibition of human HT-29 cells after 48 hrs by MTT assay Growth inhibition of human HT-29 cells after 48 hrs by MTT assay	[PMID: 33581551]
HeLa	CC₅₀	0.12 μM Compound: 8; Alvocidib	Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 27171036]
HeLa	GI₅₀	0.043 μM Compound: Flavopiridol	Growth inhibition of human HeLa cells after 48 hrs by MTT assay Growth inhibition of human HeLa cells after 48 hrs by MTT assay	[PMID: 33581551]
HeLa	IC₅₀	0.15 μM Compound: Flavopiridol	Antiproliferative activity against human HeLa cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human HeLa cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
HepG2	EC₅₀	0.1464 μM Compound: 4	Antiproliferative activity against human HepG2 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human HepG2 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
HepG2	IC₅₀	0.56 μM Compound: Flavopiridol	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
K562	IC₅₀	0.13 μM Compound: Flavopiridol	Inhibition of K562 human leukemia cell proliferation Inhibition of K562 human leukemia cell proliferation	[PMID: 12190313]
K562	IC₅₀	125 nM Compound: Alvocidib	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
KB	GI₅₀	0.16 μM Compound: FVP	Antiproliferative activity against human KB cells after 3 days by SRB method Antiproliferative activity against human KB cells after 3 days by SRB method	[PMID: 25914804]
KOPN-8	EC₅₀	0.1926 μM Compound: 4	Antiproliferative activity against human KOPN8 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human KOPN8 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
LNCaP	IC₅₀	0.016 μM Compound: Flavopiridol	Inhibition of LNCaP human prostate carcinoma cell proliferation Inhibition of LNCaP human prostate carcinoma cell proliferation	[PMID: 12190313]
LS174T	IC₅₀	0.065 μM Compound: Flavopiridol	Inhibition of LS174T human colon carcinoma cell proliferation Inhibition of LS174T human colon carcinoma cell proliferation	[PMID: 12190313]
LX-1	IC₅₀	0.075 μM Compound: Flavopiridol	Inhibition of LX-1 human lung carcinoma proliferation Inhibition of LX-1 human lung carcinoma proliferation	[PMID: 12190313]
M109	IC₅₀	0.08 μM Compound: Flavopiridol	Inhibition of M109 mouse lung carcinoma cell proliferation Inhibition of M109 mouse lung carcinoma cell proliferation	[PMID: 12190313]
MCF7	GI₅₀	0.092 μM Compound: Flavopiridol	Growth inhibition of human MCF7 cells after 48 hrs by MTT assay Growth inhibition of human MCF7 cells after 48 hrs by MTT assay	[PMID: 33581551]
MCF7	IC₅₀	0.026 μM Compound: 1	Antiproliferative activity against MCF7 cells Antiproliferative activity against MCF7 cells	[PMID: 17123821]
MCF7	IC₅₀	0.066 μM Compound: Flavopiridol	Inhibition of MCF-7 human breast carcinoma cell proliferation Inhibition of MCF-7 human breast carcinoma cell proliferation	[PMID: 12190313]
MCF7	IC₅₀	0.5 μM Compound: 1	Inhibition of MCF-7 tumor cell proliferation Inhibition of MCF-7 tumor cell proliferation	[PMID: 10843211]
MCF7	IC₅₀	0.71 μM Compound: Flavopiridol	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human MCF7 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
MDA-MB-231	IC₅₀	0.06 μM Compound: Flavopiridol	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
MDA-MB-468	GI₅₀	0.096 μM Compound: Flavopiridol	Growth inhibition of human MDA-MB-468 cells after 48 hrs by MTT assay Growth inhibition of human MDA-MB-468 cells after 48 hrs by MTT assay	[PMID: 33581551]
MIA PaCa-2	GI₅₀	0.078 μM Compound: Flavopiridol	Growth inhibition of human MIA PaCa-2 cells after 48 hrs by MTT assay Growth inhibition of human MIA PaCa-2 cells after 48 hrs by MTT assay	[PMID: 33581551]
MIA PaCa-2	IC₅₀	320 nM Compound: Alvocidib	Antiproliferative activity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human MIA PaCa-2 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
MIA PaCa-2	IC₅₀	36 μM Compound: 1 (flavopiridol)	Inhibition of Mia PaCa-2 cell clonogenic assay Inhibition of Mia PaCa-2 cell clonogenic assay	[PMID: 11063609]
MIP	IC₅₀	0.12 μM Compound: Flavopiridol	Inhibition of MIP human colon carcinoma cell line Inhibition of MIP human colon carcinoma cell line	[PMID: 12190313]
MLF	IC₅₀	0.072 μM Compound: Flavopiridol	Inhibition of MLF mouse lung fibroblast cell proliferation Inhibition of MLF mouse lung fibroblast cell proliferation	[PMID: 12190313]
MOLM-13	IC₅₀	0.07 μM Compound: Flavopiridol	Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method Antiproliferative activity against human MOLM-13 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 method	[PMID: 36087428]
MRC5	GI₅₀	0.028 μM Compound: Flavopiridol	Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay Cytotoxicity against human MRC5 cells after 72 hrs by MTT assay	[PMID: 23301767]
MRC5	GI₅₀	0.039 μM Compound: Flavopiridol	Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay Cytotoxicity against human MRC5 cells after 48 hrs by MTT assay	[PMID: 23301767]
MRC5	GI₅₀	0.049 μM Compound: Flavopiridol	Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay Cytotoxicity against human MRC5 cells after 24 hrs by MTT assay	[PMID: 23301767]
MT4	EC₅₀	0.015 μM Compound: FVP	Antiviral activity against Human immunodeficiency virus 1 NL 4-3 infected in MT4 cells measured on day 4 post infection by p24 assay Antiviral activity against Human immunodeficiency virus 1 NL 4-3 infected in MT4 cells measured on day 4 post infection by p24 assay	[PMID: 25914804]
MT4	IC₅₀	0.067 μM Compound: FVP	Cytotoxicity against human MT4 cells Cytotoxicity against human MT4 cells	[PMID: 25914804]
MV4-11	IC₅₀	0.079 μM Compound: 1	Growth inhibition of human MV4-11 cells incubated after 72 hrs by CTG- luminescence assay Growth inhibition of human MV4-11 cells incubated after 72 hrs by CTG- luminescence assay	[PMID: 34415148]
NCI-N87	IC₅₀	111 nM Compound: Alvocidib	Antiproliferative activity against human NCI-N87 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human NCI-N87 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
OVCAR-3	IC₅₀	0.054 μM Compound: Flavopiridol	Inhibition of OVCAR-3 human ovarian carcinoma cell proliferation Inhibition of OVCAR-3 human ovarian carcinoma cell proliferation	[PMID: 12190313]
Oocyte	IC₅₀	0.2 μM Compound: Flavopiridol	Inhibition of Cyclin-dependent kinase 1-cyclin B from M phase starfish (Marthasterias glacialis) oocytes Inhibition of Cyclin-dependent kinase 1-cyclin B from M phase starfish (Marthasterias glacialis) oocytes	[PMID: 12593668]
Oocyte	IC₅₀	0.4 μM Compound: Flavopiridol (table 2 Page 6846)	Inhibitory concentration against CDK1/Cyclin B complex from Marthasterias glacialis M-phase oocytes Inhibitory concentration against CDK1/Cyclin B complex from Marthasterias glacialis M-phase oocytes	[PMID: 16250643]
PC-3	IC₅₀	0.066 μM Compound: Flavopiridol	Inhibition of PC3 human prostate carcinoma cell proliferation Inhibition of PC3 human prostate carcinoma cell proliferation	[PMID: 12190313]
PC-3	IC₅₀	10 μM Compound: 1 (flavopiridol)	Inhibition of PC3 cell clonogenic assay Inhibition of PC3 cell clonogenic assay	[PMID: 11063609]
PC-3	IC₅₀	110 nM Compound: Alvocidib	Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human PC-3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
RPMI-8226	IC₅₀	140 nM Compound: Flavopiridol	Antiproliferative activity against human RPMI-8226 in presence of human serum by MTT assay Antiproliferative activity against human RPMI-8226 in presence of human serum by MTT assay	[PMID: 19169685]
RPMI-8226	IC₅₀	394 nM Compound: Flavopiridol	Antiproliferative activity against human RPMI-8226 in presence of fetal bovine serum by MTT assay Antiproliferative activity against human RPMI-8226 in presence of fetal bovine serum by MTT assay	[PMID: 19169685]
SEM	EC₅₀	0.2043 μM Compound: 4	Antiproliferative activity against human SEM cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human SEM cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
SK-BR-3	IC₅₀	0.077 μM Compound: Flavopiridol	Inhibition of SKBR-3 human breast carcinoma cell proliferation Inhibition of SKBR-3 human breast carcinoma cell proliferation	[PMID: 12190313]
SK-BR-3	IC₅₀	75 nM Compound: Alvocidib	Antiproliferative activity against human SK-BR-3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human SK-BR-3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
SK-OV-3	IC₅₀	0.22 μM Compound: Alvocidib	Antiproliferative activity against human SKOV3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay Antiproliferative activity against human SKOV3 cells assessed as reduction in cell viability incubated for 72 hrs by cell titer-glo luminescent assay	[PMID: 32402934]
SUP-B15	EC₅₀	> 86.2069 μM Compound: 4	Antiproliferative activity against human SUP-B15 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human SUP-B15 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]
Sf21	IC₅₀	395 nM Compound: 8; Alvocidib	Inhibition of full length human N-terminal His6-tagged CDK6/N-terminal GST-tagged cyclin D3 expressed in sf21 cells using histone H1 substrate Inhibition of full length human N-terminal His6-tagged CDK6/N-terminal GST-tagged cyclin D3 expressed in sf21 cells using histone H1 substrate	[PMID: 27171036]
Sf9	IC₅₀	0.012 μM Compound: flavopiridol	Inhibition of recombinant cyclin A/CDK2 expressed in Sf9 cells Inhibition of recombinant cyclin A/CDK2 expressed in Sf9 cells	[PMID: 17904366]
Sf9	IC₅₀	0.13 μM Compound: FVP	Inhibition of CDK2/cyclin E1 (unknown origin) expressed in Sf9 insect cells using UlightCFFKNIVTPRTPPPSQGK-amide substrate after 15 mins by autoradiography Inhibition of CDK2/cyclin E1 (unknown origin) expressed in Sf9 insect cells using UlightCFFKNIVTPRTPPPSQGK-amide substrate after 15 mins by autoradiography	[PMID: 25914804]
Sf9	IC₅₀	2.5 nM Compound: 8; Alvocidib	Inhibition of human His6-tagged CDK9/cyclin T1 expressed in baculovirus infected sf9 cells using GST-CTD as substrate after 10 mins in presence of [gamma-32P]ATP by SDS-PAGE analysis Inhibition of human His6-tagged CDK9/cyclin T1 expressed in baculovirus infected sf9 cells using GST-CTD as substrate after 10 mins in presence of [gamma-32P]ATP by SDS-PAGE analysis	[PMID: 27171036]
T-cell	IC₅₀	20 nM Compound: 1; L868275	Inhibition of CDK9/Cyclin T (unknown origin) Inhibition of CDK9/Cyclin T (unknown origin)	[PMID: 35485642]
T-cell	IC₅₀	6 nM Compound: Flavopiridol	Inhibition of CDK9/Cyclin T (unknown origin) Inhibition of CDK9/Cyclin T (unknown origin)	[PMID: 19169685]
UoC-B1	EC₅₀	0.2084 μM Compound: 4	Antiproliferative activity against human UOCB1 cells after 72 hrs by CelTiter-Glo assay Antiproliferative activity against human UOCB1 cells after 72 hrs by CelTiter-Glo assay	[PMID: 29407975]

体外研究
(In Vitro)

Flavopiridol (2 μM) robustly induces a distinct pattern of ER stress in CLL cells that contributes to cell death through IRE1-mediated activation of ASK1 and possibly downstream caspases^[1]. Flavopiridol results in potent upregulation of a number of PRGs in treatments lasting 4-24 h. Flavopiridol has and immediate and long-term effect on the expression of several PRGs. In serum starved cells re-stimulated with serum, flavopiridol also inhibits the expression of these genes, but subsequently, JUNB, GADD45B and EGR1 are upregulated in the presence of flavopiridol^[2].
Flavopiridol (Alvocidib) also inhibits cyclin E1 and induces apoptosis^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

401.84

Formula

C₂₁H₂₀ClNO₅

CAS 号

146426-40-6

性状

固体

颜色

Light yellow to yellow

中文名称

夫拉平度

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 2 years; -20°C, 1 year (stored under nitrogen)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 33.33 mg/mL (82.94 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4886 mL	12.4428 mL	24.8855 mL
5 mM	0.4977 mL	2.4886 mL	4.9771 mL
10 mM	0.2489 mL	1.2443 mL	2.4886 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year (stored under nitrogen)。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.22 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.22 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 2 years; -20°C, 1 year (stored under nitrogen)

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.73%

选择批次：

Data Sheet (630 KB) SDS (393 KB)

COA (293 KB) HNMR (254 KB) RP-HPLC (242 KB) LCMS (93 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Mahoney E, et al. ER stress and autophagy: new discoveries in the mechanism of action and drug resistance of the cyclin-dependent kinase inhibitor flavopiridol.Blood. 2012 Aug 9;120(6):1262-1273. [Content Brief]

[2]. Keskin H, et al. Complex effects of flavopiridol on the expression of primary response genes. Cell Div. 2012 Mar 29;7:11. [Content Brief]

[3]. Kim KS, et al.Thio- and oxoflavopiridols, cyclin-dependent kinase 1-selective inhibitors: synthesis and biological effects. J Med Chem. 2000 Nov 2;43(22):4126-34. [Content Brief]

[4]. Jianliang Xu, et al. Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression. Cell Commun Signal. 2019 Jul 26;17(1):85. [Content Brief]

Cell Assay ^[1]	The cells treated with flavopiridol are washed after 4 hours with PBS and resuspended in regular growth medium (RPMI 1640) supplemented with 10% human serum and antibiotics for the remainder of the incubation time. In the case of flavopiridol/NSC-187208 samples, NSC-187208 is re-added in the fresh media after flavopiridol is washed at 4 hours. For all the other conditions, cells are incubated with the respective drugs for 24 hours continuously. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Mahoney E, et al. ER stress and autophagy: new discoveries in the mechanism of action and drug resistance of the cyclin-dependent kinase inhibitor flavopiridol.Blood. 2012 Aug 9;120(6):1262-1273. [Content Brief] [2]. Keskin H, et al. Complex effects of flavopiridol on the expression of primary response genes. Cell Div. 2012 Mar 29;7:11. [Content Brief] [3]. Kim KS, et al.Thio- and oxoflavopiridols, cyclin-dependent kinase 1-selective inhibitors: synthesis and biological effects. J Med Chem. 2000 Nov 2;43(22):4126-34. [Content Brief] [4]. Jianliang Xu, et al. Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression. Cell Commun Signal. 2019 Jul 26;17(1):85. [Content Brief]

Cell Assay
^[1]

The cells treated with flavopiridol are washed after 4 hours with PBS and resuspended in regular growth medium (RPMI 1640) supplemented with 10% human serum and antibiotics for the remainder of the incubation time. In the case of flavopiridol/NSC-187208 samples, NSC-187208 is re-added in the fresh media after flavopiridol is washed at 4 hours. For all the other conditions, cells are incubated with the respective drugs for 24 hours continuously.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Mahoney E, et al. ER stress and autophagy: new discoveries in the mechanism of action and drug resistance of the cyclin-dependent kinase inhibitor flavopiridol.Blood. 2012 Aug 9;120(6):1262-1273. [Content Brief]

[2]. Keskin H, et al. Complex effects of flavopiridol on the expression of primary response genes. Cell Div. 2012 Mar 29;7:11. [Content Brief]

[3]. Kim KS, et al.Thio- and oxoflavopiridols, cyclin-dependent kinase 1-selective inhibitors: synthesis and biological effects. J Med Chem. 2000 Nov 2;43(22):4126-34. [Content Brief]

[4]. Jianliang Xu, et al. Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression. Cell Commun Signal. 2019 Jul 26;17(1):85. [Content Brief]

Flavopiridol 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4886 mL	12.4428 mL	24.8855 mL	62.2138 mL
	5 mM	0.4977 mL	2.4886 mL	4.9771 mL	12.4428 mL
	10 mM	0.2489 mL	1.2443 mL	2.4886 mL	6.2214 mL
	15 mM	0.1659 mL	0.8295 mL	1.6590 mL	4.1476 mL
	20 mM	0.1244 mL	0.6221 mL	1.2443 mL	3.1107 mL
	25 mM	0.0995 mL	0.4977 mL	0.9954 mL	2.4886 mL
	30 mM	0.0830 mL	0.4148 mL	0.8295 mL	2.0738 mL
	40 mM	0.0622 mL	0.3111 mL	0.6221 mL	1.5553 mL
	50 mM	0.0498 mL	0.2489 mL	0.4977 mL	1.2443 mL
	60 mM	0.0415 mL	0.2074 mL	0.4148 mL	1.0369 mL
	80 mM	0.0311 mL	0.1555 mL	0.3111 mL	0.7777 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flavopiridol146426-40-6HMR-1275 Alvocidib L86-8275夫拉平度HMR1275HMR 1275CDKAutophagyHIVApoptosisCyclin dependent kinaseHuman immunodeficiency virusInhibitorinhibitorinhibit

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Flavopiridol (Synonyms: 夫拉平度; HMR-1275; Alvocidib; L86-8275)

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Flavopiridol (Synonyms: 夫拉平度; HMR-1275; Alvocidib; L86-8275)

Customer Review

Other Forms of Flavopiridol:

Flavopiridol 相关抗体

MCE 顾客使用本产品发表的 52 篇科研文献

Flavopiridol 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 CDK 亚型特异性产品:

查看 HIV 亚型特异性产品:

生物活性

实验参考方法

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参考文献

Flavopiridol 相关抗体:

摩尔计算器

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Flavopiridol 相关分类

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