1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-γ
  5. IFN-gamma 蛋白, Mouse (155a.a, HEK293)

IFN-gamma 蛋白, Mouse (155a.a, HEK293)

目录号: HY-P73251
产品使用指南

IFN-γ 蛋白是一种 II 型干扰素,是一种由免疫系统细胞分泌的可溶性细胞因子,作为同二聚体结合干扰素γ受体并触发针对感染的细胞反应。缺乏 IFN-γ 的小鼠对各种感染的易感性更高,患自身免疫性疾病的风险也更高。在参考数据集中,IFN-gamma 表现出低表达水平。IFN-gamma 蛋白, Mouse (155a.a, HEK293) 是重组的 IFN-γ 蛋白,由 HEK293 表达,不带标签。IFN-gamma 蛋白, Mouse (155a.a, HEK293) 全长 133 个氨基酸,分子量分别约为 ~17.6 & 21.7 kDa。

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描述

IFN-gamma protein, a type II interferon, is a soluble cytokine secreted by cells in the immune systems, acting as a homodimer to bind the interferon gamma receptor and trigger a cellular response against infections. Mice deficient in IFN-gamma display heightened susceptibility to various infections and an increased risk of autoimmune diseases. In the reference dataset, IFN-gamma exhibits low expression levels. IFN-gamma Protein, Mouse (155a.a, HEK293) is the recombinant mouse-derived IFN-gamma protein, expressed by HEK293 , with tag free. The total length of IFN-gamma Protein, Mouse (155a.a, HEK293) is 133 a.a., with molecular weight of ~17.6 & 21.7 kDa, respectively.

研究背景

IFN-γ蛋白是II型干扰素类的一员,是先天性和适应性免疫系统细胞分泌的可溶性细胞因子。它的活性形式以同二聚体形式存在,与干扰素γ受体结合,启动针对病毒和微生物感染的细胞反应。缺乏该基因的小鼠表现出对病毒、细菌和寄生虫感染的易感性增加,以及多种自身免疫性疾病的风险增加。在参考数据集中,IFN-gamma 表现出低表达水平。

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

NP_032363.1 (H23-C155)

基因 ID

15978  [NCBI]

蛋白结构
N-term
IFN-gamma (H23-C155)
Accession # NP_032363.1
C-term
同用名
IFG; IFI; IFNG; IFN-gamma; Immune interferon; Interferon gamma
氨基酸序列

HGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC

分子量

Approximately 17.6&21.7 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IFN-gamma Protein, Mouse (155a.a, HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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